ObjectiveTo observe the effect of compound chlorhexidine gargle wash care for patients after radical surgery of tongue cancer. MethodsBetween January 2013 and March 2014, 40 patients with tongue cancer who underwent radical surgery without radiation therapy or chemotherapy before operation were selected and randomly divided into compound chlorhexidine giuconatie gargle solution group (intervention group, n=19) and traditional oral care group (control group, n=21). Then we compared the two groups in terms of bacterial colony number, oral cavity cleanness, incidence rate of bad breath and oral ulcer. ResultsBefore intervention, there was no significant diTherences between the two groups in the number of bacterial colony, oral cavity cleanness or oral odor (P>0.05). After treatment, the bacterial colony number and incidence of oral ulcer in the intervenient group were significantly lower, and oral odor was slighter than that of the control group (P<0.05). Oral cavity cleanness between the two groups was not significantly different (P>0.05). ConclusionCompound chlorhexidine gargle wash care for patients after radical surgery of tongue cancer was better than the traditional treatment in terms of bacterial colony number, incidence rate of bad breath and oral ulcer. It is worth clinical popularizing.
ObjectiveTo study the effect of Triton X-100 promoting liposome-mediated bone morphogenetic protein 2 (BMP-2) gene transfection of rat bone marrow mesenchymal stem cells (BMSCs). MethodsBMSCs were separated and cultured from the femur and tibia of healthy Wistar rats (8-week-old, male). The 3rd passage BMSCs identified by detecting the surface antigen were used to transfect. The optimum concentration of Triton X-100 for liposome mediated gene transfection was determined with ELISA meter by the way of MTT. In optimum concentration of Triton X-100, liposome mediated BMP-2 gene was transfected to BMSCs. The experiment was divided into 3 groups according to types of trasfection agents:BMSCs were transfected with Triton X-100+liposome+BMP-2 (experimental group), with liposome+ BMP-2 (conventional transfection group), and untransfected BMSCs served as blank control group. After 48 hours of transfecting, the green fluorescent protein (GFP) in cells was detected through inverted fluorescence microscope. After 72 hours of transfection, real-time fluorescence quantitative PCR was applied to measure the mRNA expression of BMP-2. Results0.01% Triton X-100 was determined to be the optimum concentration for not only making the BMSCs maintain vitality, but also achieving a certain effect on BMSCs. After trasfecting for 48 hours, GFP was observed through inverted fluorescence microscope in the experimental group and conventional transfection group, but was not observed in the blank control group. After trasfecting for 72 hours, the relative BMP-2 mRNA expression level was 5.94±0.12 in the experimental group, and was 4.99±0.08 in the conventional transfection group, showing significant difference (t=360.28, P=0.02). The transfection efficiency was increased by 19% in the experimental group. Conclusion0.010%Triton X-100 can promote the liposome mediated BMP-2 gene transfection of rat BMSCs, and can improve the transfection efficiency.