An experimental model of retinal acute photic injury was developed in Wister rats. Aninmls were exposed to white light in intensity of 20 000 lux under general anesthesia for 1 hr. Two rats were sacrificed at 24, 48hr, day 7, 14,21,28,35 after liht exposure respectively. The histopathologic study showed that the retinal acute photic injury initiated in the outer seSment characterized by disorganization and loss of the outer segment at the early stage, and then the inner segment and RPE were involved. The decrease of the thickness of the outer nuclear layer was seen in a few of our cases. Some of our samples showed recovery of the outer and inner segment from the light damage of certian degrees 2 or 3 weeks after light exposure, but others did not. One sample from 5 weeks after light exposure demonstrated that the outer nuclear layer, the outer and inner segment completely lost. Thc inflammatory responses were not observed in all of our samples inplicating that the retinal acute photic damage is a degeneration process. （Chin J Ocul Fundus Dis,1994,10:84-85）
Objective To study the progressive development of the retinas through an observation on the histological changes of the retinas from neonatal mice of different day-ages. Methods The retinas from the mice of 1 to 20 days of age were examined by light microscopy,and from 1 to 3 days,by autoradiography. Results The retinas of the mice below 3 days of age only had the RPE cells layer,the neuroblast layer and the ganglion cell layer.With the increase in dayage,the retinas developed gradually and would be mature in the 20th day. Conclusions The retinas of mice is a kind of immature tissue before the 20th days,so it can be considered as transplantation donors. (Chin J Ocul Fundus Dis, 1999, 15: 174-176)
Purpose To observe the pathologic changes of retinal photic injury in mice. Methods A light damaged trunk was designed by ourselves.The mice were given an intermitent light exposure for 3 days,12 hours light exposure every day and 12 hours dark adaption before every exposure.Experimental animals were sacrificed on the 1st,6th,12th,18th and 30th day after light injury,and the eyes were enucleated for light and electronic microscopy observation. Results The early pathologic changes including disc membrance swelling,disorganization in outer segments,and mitochondrial swelling,spherical change in inner segments．Then the chromatin densification,liquefaction and margination,and the shrinkage of nuclear membrance were found in the nuclear layer.Finally the outer nuclear layer became thin and disappeared.The apical microvill of RPE cell disappeared and basic fold became flat in some samples. Conclusion The photoreceptor degeneration was the pathologic characteristic of retinal photic injury in mice. (Chin J Ocul Fundus Dis,1998,14:215-218)
OBJECTIVE:To examine the possibility of allogenic neural retinal transplantation. METHODS:Donor neural retinal tissue obtained from neonatal mice was implanted into the subretlnal space of 36 adult mice. Twelve recipients were sacrificed at day 6,day 12,and day 18 post-transplantation respectively, and the eyes were enucleated for histologic examination. RESULTS: The graft was failed to be found but the host retina remained normal in 16.7%(6/36),the graft was found survival and showed further differentiation into rosette formation in the host subretinal space and inner retina in 66.7%(24/36),in the vitreous cavity in 8.3% (3/36). Disastrous granulomatous inflammation occurred in 8.3%(3/36). CONCLUSION: Allogenic retina from neonatal mice implanted into the subretinal space might experience immune privilege .in a great extent ,and showed further differentiation during the experimental periods of time. （Chin J Ocul Fundus Dis,1996,12: 236-238 ）