ObjectiveTo evaluate the influence of different surgical procedure on the prognosis of elderly patients with stage Ⅰ non-small cell lung cancer (NSCLC). MethodsWe retrospectively analyzed the clinical data of 100 patients elder than 70 years with NSCLC in our hospital between January 2012 and December 2013. There were 60 males and 40 females patients at age of 70-85 (76±12) years. The patients were divided into two groups including a standard lobectomy group (62 patients, receiving standard lobectomy) and a limited resection group (38 patients receiving limited resection). Regular following-up was carried out after the surgery. The correlation between the survival rate and the incidences of complications, the surgical procedure were analyzed. ResultsThe incidence of complications in the standard lobectomy group and the limited resection group was 35.5%(22/62)and 39.5%(15/38)respectively. The three year survival rate and the five year survival rate in the two groups were similar between the two groups(85.5% versus 54.8% and 78.9% versus 55.3%). There was no statistical difference between the two groups. Staging is an independent factor for prognosis. ConclusionLobectomy is still the main therapy method for elderly stageⅠNSCLC patients. But limited resection also appears to be a viable surgical treatment for patients with cardiopulmonary physiologic impairment.
This paper aims to study the effects of traditional Chinese medicine Euphorbia esula on multidrug resistant human gastric cancer cells in the cell proliferation, migration, invasion and apoptosis, and to study the apoptosis-inducing pathway. Different dilutions of Euphorbia esula extract were used to process human multidrug resistant gastric cancer SGC7901/ADR cells. Cell proliferation inhibition phenomenon was determined by MTT experiment. Nuclear morphological changes of apoptotic cells and apoptotic indexes were observed and determined by Hochest33528 staining followed with fluorescence microscope observing. Flow cytometry was used to detect cell apoptosis rate. Cell migration and invasion ability were observed and determined by Transwell method. Spectrophotometry was used to detect caspase-3 and caspase-9 enzyme activity. Western blotting was used to detect subcellular distribution of cytochrome c. The results showed that Euphorbia esula extract had obvious inhibition effect on proliferation of gastric cancer multidrug resistant SGC7901/ADR cells, which was time- and concentration-dependent. After processing multidrug resistant gastric cancer SGC7901/ADR cells with Euphorbia esula extract, the apoptotic index and apoptosis rate were significantly increased than those in the control group, which showed a time- and dose-dependent mode; but if a caspase inhibitor was added, apoptosis index was not obviously increased. Transwell method showed that migration and invasion ability of the Euphorbia esula extract-processed SGC7901/ADR cells dropped significantly. Spectrophotometry showed that in Euphorbia esula extract-processed SGC7901/ADR cells, caspase-3 and caspase-9 expression were increased, which had significant differences with the control group. Western blotting test showed that the distribution of cytochrome c decreased in mitochondria, while increased in the cytoplasm (i.e., cytochrome c escaped from mitochondria to the cytoplasm). In conclusion, Euphorbia esula extract could inhibit the proliferation, migration and invasion, and induce apoptosis in human gastric cancer multidrug resistant SGC7901/ADR cells; and cytochrome c, caspase-9 and caspase-3 might be involved in cell apoptosis induced by Euphorbia esula extract, suggesting endogenous or mitochondrial apoptotic pathway.