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find Author "何守志" 9 results
  • 基因芯片技术在眼底病研究中的应用

    将大量基因片段或寡核苷酸有序、高密度排列在玻璃、硅等载体上,称之为基因芯片。基因芯片技术以其检测快速、高效、高通量、高度并行性、微型化和自动化等特点,成为了研究生命本质及疾病发生发展规律的重要手段。现对其基本概念、特点、基本原理及其在眼底病研究中的应用前景作一综述。 (中华眼底病杂志,2004,20:265-266)

    Release date:2016-09-02 05:58 Export PDF Favorites Scan
  • Photodynamic therapy for choroidal neovascularization in age-related macular degeneration

    Objective To evaluate short-term effects of a single photodynamic therapy (PDT) treatment with visudyne (CIBA Vision Corp.) for choroidal neovascularization (CNV) in age-ralated macular degeneration (AMD). Methods Thirty cases (35 eyes) diagnosed as AMD patients with classic CNV were treated with PDT. The data of visual acuity testing, fluorescein angiography (FFA), indocyanine green angiography (ICGA) and optic coherence tomography (OCT) were used to evaluate the effects of a single treatment of PDT before and 1 week, 1 ,3 month after treatment. Results The visual acuity of 34 eyes were stable or improved in 3 months follow-up;and the visual acuity of 1 eye was decreased. Decrease or dispearance of fluorescein leakage from CNV was noted in 19 eyes. No serious complication occurred. Conclusion Single treatment of PDT for CNV in AMD can achieve short-term decrease or cessation of fluorescein leakage from CNV without loss of visual acuity. (Chin J Ocul Fundus Dis, 2002, 18: 171-174)

    Release date:2016-09-02 06:01 Export PDF Favorites Scan
  • The effect of methylprednisolone on the expression of glial fibrillary acidic protein and proliferating cell nuclear antigen in Müller cells of rats’ retinae injured by laser

    Objective To investigate the effect of methylprednisolone on the expression of glial fibrillary acidic protein (GFAP) and proliferating cell nuclear antigen (PCNA) in Müller cells of rats’ retinae injured by laser. Methods Forty SD rats were randomly divided into two groups and inflicted with laser photocoagulation.The rats in treatment group were given methylprednisolone by intraperitoneal injection with a dose of 30 mg/kg for 3 days.At the 3rd,7th,14th,and 28th day after photocoagulation respectively, the eyes were enucleated,fixed and cut into sections.Immunohistochemical examination was used to detect the expression of PCNA and GFAP. Results After photocoagulation the Müller cells expressed PCNA both in the treatment and control group,and the expression of PCNA decreased sharply after 3 days. The expression of PCNA in treatment group was less than that in control group. After photocoagulation the Müller cells also expressed GFAP and the expression of GFAP lasted for at least 28 days ,and the expression of GFAP expression in the treatment group was less than that in the control group. Conclusion Methylprednisolone can reduce the expression of GFAP and PCNA in Müller cells of rats’ retinae injured by laser. (Chin J Ocul Fundus Dis, 2002, 18: 299-301)

    Release date:2016-09-02 06:01 Export PDF Favorites Scan
  • 氩离子激光治疗脉络膜骨瘤二例三眼

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  • 雷公藤多甙对兔眼人工晶体植入后虹膜睫状体炎症反应的影响

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  • Alginate sodiumretinoic acid microspheres suppress the subretinal proliferation after laser coagulation

    Objective To observe the inhibiting effects of alginate sodiumretinoic acid(AGS-RA)microspheres release system on the laser coagulationinduced subretinal proliferation.Methods RA were dissolved by absolute alcohol,then mixed with 1.5% AGS and made into AGSRA microspheres by a microcapsule electrostatic generator. The parameter of laser injury include irradiation time (0.20 s),spot diameter (200 mu;m) and output power (420 mW).Thirty pigmented rabbits were randomly divided into 3 groups (laser injury,experimental and control group).After laser coagulation,AGSRA or blank microspheres were immediately injected into the vitrous of experimental and control rabbits respectively.The height,width and area of 6 retinal spots of laser coagulation at each timepoint were analyzed histopathologically with serial retinal sections at 1,2,3,4,and 6 weeks after laser coagulation.Results Histopathological examination showed that there were morphological and distribution changes of retinal cells in all layers, and localized fibroblasts proliferation in the retina after laser injury. The laserinduced responses in experimental group were much milder(P<0.01), while the laser injury group and control group have same width(P>0.05)and height/area of laser spots(P>0.05).Conclusion AGSRA release system can alleviate the subretinal proliferate after laser injury.

    Release date:2016-09-02 05:42 Export PDF Favorites Scan
  • Clinical observation of intravitreal injection of ranibizumab for 12 consecutive months treating exudative age-related macular degeneration

    Objective T o observe the efficacy and safety of intravitreal injection of ranibizumab (Lucentis) for 12 consecutive months treating exudative age-related macular degeneration (EAMD). Methods This is an open and prospective study without control trial. Twenty-two eyes from 22 patients (18 males and 4 females) with EAMD diagnosed by fundus fluorescein angiography (FFA) and indocyanine green angiography (ICGA) were enrolled in this study. The patients aged from 46 to 79 years with the mean of 68.2plusmn;9.3 years. All of the patients received intravitreal injection of ranibizumab (0.5 mg, 0.05 ml) once a month for 12 consecutive months. The best-corrected visual acuity (BCVA) was obtained using the international standard visual acuity chart (converted into logMAR for statistical analysis). Central retinal thickness (CRT) was measured by optical coherence tomography (OCT) before and after each monthly treatment. Intraocular pressure (IOP) was measured using non-contact tonometry before treatment and 1 hour and 1 day after treatment. FFA and ICGA were performed every 3 months. The BCVA was 0.01 -0.9 with the average of 0.26plusmn;0.22. The average logMAR BCVA was 0.76plusmn;0.44, the CRT was 182-559 mu;m with the mean of (302.62plusmn;90.18) mu;m, and the IOP was normal before treatment. Only 13 of 22 patients completed 12 months of follow-up. The BCVA, CRT and IOP after treatment were compared with baseline using paired t test. Results Of all the 22 patients, the mean logMAR BCVA at 1 and 3 months after treatment were 0.52plusmn;0.32 and 0.37plusmn;0.27 respectively, both of which were significantly different compared with before treatment (t=4.518, 6.237;P<0.05). Of the 13 patients, the mean logMAR BCVA at 1, 3 and 12 months after treatment was 0.51plusmn;0.34, 0.35plusmn;0.26, 0.34plusmn;0.30 respectively. All of these measures were significantly different compared with 0.74plusmn;0.37 before treatment (t=3.443, 5.438, 4.756;P<0.05) . The mean CRT at 1, 3 and 12 months after treatment were (228.85plusmn;54.93), (231.00plusmn;38.94) and (262.92plusmn;70.48) mu;m. There were significant differences among before and 1, 3 months after treatment (t=2.914, 3.199;P<0.05), but not between before and 12 months after treatment (t=1.408, P>0.05). The first 3 injections contributed to the most BCVA gain with 6 patientsprime; BCVA increasing from 0.1- 0.5 to 0.5 or above. The greatest CRT reduction was obtained at 1 month after the first injection. IOP increased 1 hour after treatment and recovered within 1 day. No intravitreal injection-related side effects such as endophthalmitis were observed during the follow-up period. Conclusions Monthly intravitreal injections of ranibizumab may improve BCVA and macular edema. There was no adverse event during the follow-up duration.

    Release date:2016-09-02 05:37 Export PDF Favorites Scan
  • 雷公藤多甙对兔眼人工晶体植入后虹膜睫状体超微结构变化的影响

    Release date:2016-09-02 06:11 Export PDF Favorites Scan
  • 外伤性白内障摘除及人工晶体植入术后虹膜和睫状体透射电镜观察

    Release date:2016-09-02 06:12 Export PDF Favorites Scan
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