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find Author "刘小方" 4 results
  • Joint Detection of hTERT mRNA and GGT mRNA-H Expressions in Peripheral Blood of Small Hepatocellular Carcinoma Patients and Its Clinical Significance

    ObjectiveTo investigate the expressions and clinical significance of human telomerase reverse transcriptase (hTERT) mRNA and γglutamyl transpeptidase mRNA-H (GGT mRNA-H) in the peripheral blood of small hepatocellular carcinoma (HCC) patients. MethodsThe expressions of hTERT mRNA and GGT mRNA-H were detected in the peripheral blood of thirty patients with small HCC by RT-PCR, eighteen patients with benign liver diseases, and twelve normal volunteers. ResultsThe positive rate of hTERT mRNA and GGT mRNA-H expression in patients with small HCC were 80.0% (24/30) and 46.7%(14/30), respectively. In patients with hepatitic cirrhosis the positive rate of hTERT mRNA expression was 33.3% (6/18), while the expression of GGT mRNA was not detected. Both the expressions of hTERT mRNA and GGT mRNA-H were negative in all normal volunteers. The combination analysis of hTERT mRNA and GGT mRNA-H expression achieved positive rate of 86.7% in the diagnosis of small HCC, which was significantly higher than the positive rate of AFP (26.7%), Plt;0.05. ConclusionThe hTERT mRNA and GGT mRNA-H are significantly expressed in small HCC patients, the combination analysis of hTERT mRNA and GGT mRNA-H seems to be useful in the early diagnosis of small HCC.

    Release date:2016-09-08 10:46 Export PDF Favorites Scan
  • 胆囊神经纤维肉瘤1例报道△

    Release date:2016-09-08 10:35 Export PDF Favorites Scan
  • 胰腺平滑肌肉瘤的诊治分析(附1例报道)

    患者,女,51岁,因“查体发现胰尾占位性病变1周”入院。入院查体: 一般情况可,神志清,查体合作,全身浅表淋巴结无肿大。心、肺听诊未见异常。腹部平坦,无胃肠形、蠕动波,无腹壁静脉曲张,全腹无压痛及反跳痛,Murphy征阴性,肝、脾未触及,移动性浊音阴性,肠鸣音正常。左上腹可触及一大小约7.0 cm×6.0 cm的包块,边界清楚,质韧……

    Release date:2016-09-08 10:58 Export PDF Favorites Scan
  • Promoter Hypermethylation of DNA Repair Gene MGMT in Cholangiocarcinoma

    ObjectiveTo explore the clinical significance of promoter hypermethylation of O6-methylguanine-DNA methyltransferase (MGMT) in cholangiocarcinoma. MethodsPromoter methylation status of MGMT gene and expression of MGMT protein were detected in cholangiocarcinoma by methylationspecific PCR and immunohistochemical staining, respectively. ResultsAberrant methylation of MGMT gene was detected in 17 patients (47.2%). Twentyone cases showed negative immunoreactivities. Of 21 patients with negative MGMT expression, 14 patients had aberrant methylation of MGMT gene. In 15 patients with positive MGMT expression, aberrant methylation of MGMT gene was only found in three cases. There was a negative correlation between promoter methylation status of MGMT gene and the expression of MGMT protein (rs=-0.816, Plt;0.05). Promoter methylation status of MGMT gene was related to depth of invasion, degree of differentiation, and TNM stage (Plt;0.05), but not to age of patient, gender, pathological type, and lymph node metastasis (Pgt;0.05). ConclusionsHypermethylation of MGMT promoter is a frequency molecular event in cholangiocarcinoma and may be involved in carcinogenesis. Methylation status of MGMT gene may be used to evaluate malignant degree of cholangiocarcinoma.

    Release date:2016-09-08 10:42 Export PDF Favorites Scan
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