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find Author "刘晓东" 2 results
  • 经屈氏韧带入路行腹腔镜右半结肠切除术: 附1例结肠癌报道

    目的介绍经屈氏韧带入路的腹腔镜右半结肠切除术的可行性和安全性。方法回顾性分析青岛大学附属医院胃肠外科收治的1例升结肠癌并行腹腔镜下右半结肠切除术患者的手术信息,该术式优先经屈氏韧带入路进行手术操作快速、准确地进入十二指肠胰头前间隙并确定外科层面,进行胰十二指肠上方、下方和外侧Toldt间隙拓展。结果该例患者的手术Toldt间隙游离并淋巴结清扫时间55 min,整个手术时间90 min,术中出血量为约20 mL,未输血,术后无消化道出血、吻合口漏、粘连性肠梗阻等并发症发生,结合加速康复外科举措的运用,于术后第6天康复出院。结论经屈氏韧带入路是一种新的腹腔镜下右半结肠切除术的入路方式,具有解剖精准、导向清晰、操作快速、安全、符合肿瘤根治原则等列优点。

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  • EFFECT OF DIFFERENT CONCENTRATIONS OF DEXAMETHASONE ON APOPTOSIS AND EXPRESSION OF FAS/FASL IN HUMAN OSTEOARTHRITIS CHONDROCYTES

    Objective Corticosteroids can destroy the cartilage. To investigate the effect of dexamethasone (Dexa) on the apoptosis and expression of Fas/FasL of human articular chondrocytes (HACs) in vitro so as to explore the mechanism ofpro-apoptotic role of Dexa on HACs. Methods Following full agreement of patients, the cartilage specimens were collectedfrom the patients with osteoarthritis undergoing knee replacement. The second passage HACs were incubated in cell culture media containing 0.125, 1.25, 12.5, 25, and 50 μg/mL Dexa for 48 hours respectively to determine the optimal concentration of Dexa by MTT. The apoptosis was assessed by TMRE/Hoechst/Annexin V-FITC/7-AAD quadruple staining after culture for 0, 24, and 48 hours. The mRNA expressions of Fas and FasL were determined by real-time quantitative PCR after culture for 48 hours. The protein expressions of Fas and FasL were determined by immunohistochemistry staining analysis after culture for 24 hours and 48 hours. Results The cell inhibitory rate of 25 μg/mL Dexa was significantly higher than that of 50 μg/mL Dexa (P lt; 0.05), and there were significant differences when compared with that at other concentrations of Dexa (P lt; 0.05), so 25 μg/mL Dexa was appropriately selected as an optimal concentration of Dexa. The apoptotic rates of HACs were 5.8% ± 0.3%, 27.0% ± 2.6%, and 36.0% ± 3.1% at 0, 24, and 48 hours, respectively, in a time dependent manner (P lt; 0.05). The expressions of Fas mRNA were (8.93 ± 1.12) × 10—3 in the experimental group and (3.31 ± 0.37) × 10—3 in the control group, showing significant difference (P lt; 0.05). The expressions of FasL mRNA were (5.92 ± 0.66) × 10—3 in the experimental group and (2.31 ± 0.35) × 10—3in the control group, showing significant difference (P lt; 0.05). The expressions of Fas and FasL proteins showed an increasing tendency with time in the experimental group and the expressions were significantly higher than those in the control group after culture for 24 hours and 48 hours (P lt; 0.05). Conclusion Dexa can induce the apoptosis and significantly upregulate the apoptotic gene expression of Fas/FasL, which can provide the experimental evidence to further investigate the role of Fas/FasL signaling pathway in Dexa-induced HACs apoptosis.

    Release date:2016-08-31 04:23 Export PDF Favorites Scan
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