ObjectiveTo explore the dynamic changes of microvessels in the hippocampal CA3 area in mice model of temporal lobe epilepsy (TLE) induced by pilocarpine. MethodsEighteen health SPF male C57BL/6 mice were randomly divided into control group and status epilepticus (SE) group. The SE group was subdivided into three groups:SE-7 days, SE-28 days and SE-56 days. SE was induced by intraperitoneal injection of pilocarpine. And immunohistochemical staining was used to detected the localization of platelet endothelial cell adhesion molecule-1 (PECAM-1). ResultsIn the control group, PECAM-1 labeled microvessels arranged in a layered structure, and the microvessel of the orient layer was most prominent. After SE, the microvessels started to form an unorganized vascular plexus and appeared fibrous and fragmented, which was prominent at SE-28 days. Furthermore, the microvessels density increased the top at SE-28 days compared to the control (P < 0.001). ConclusionThe angiogenesis exists during the hippocampus formation in the mice model of TLE induced by pilocarpine, which could direct a new explanation for TLE formation and development.
Objectives The purpose of this study is to verify the phenytoin-resistant mesial temporal lobe epilepsy (MTLE) induced by Li-pilocarpine and screened by antiepilepsy drug (AEDs). Methods The rats with MTLE were induced by Li-pilocarpine, which were screened by effect of phenytoin treatment monitored by vedio-EEG. The living microdialysis technology was used for verification of drug concentration in brain of drug-resistant and drug-responsive rat model, and the P-glycoprotein expression was detected by immunohistochemical method. Results Sixteen rats with chronic MTLE were successfully induced in total 30 rats, among which, 6 drug-resistant rats with MTLE were screened. The brain/plasma ratio of area under the curve in drug-resistant rats was significantly lower than that of drug-responsive rats (0.15±0.03 vs. 0.28±0.05, P<0.05). In addition, the P-glycoprotein expression in brain of drug-responsive rats was obviously higher than that of drug-responsive rats (P<0.05). Conclusions The low concentration of phenytoin in drug-resistant rat model with MTLE was verified that might be related to the over-expressed P-glycoprotein in brain.
ObjectiveTo observe the effects of Tianjiang Niuhuang Powder on cognition, seizure and hippocampal formation in epileptic model of pilocarpine rats.MethodsFouty SD rats were selected in this study. They were randomly divided into four groups (each group n=10), including a blank control group (group A) and three pilocarpine epilepsy model groups. The model groups were divided into Tianjiang Niuhuang Powder treatment Group (group B), Sodium valproate (group C) and model control group (group D) according to the different processing methods. The seizures number of discharges in the EEG monitor, cognitive ability were observed on the 7th, 14th and 21st day, The pathological sections of the brain and the neuronal damage in the hippocampal CA1 area were observed after the rats were sacrificed.ResultsThere was no significant difference in the number of spontaneous episodes, duration, and average number of discharges per minute between group B and group C (P>0.05). Compared with groups B and C, group D increased the number of episodes and increased duration. The number of discharges increased, the difference was statistically significant (P<0.05). Group A had no seizures. There were no significant differences in cognitive ability between groups A, B and C (P>0.05). Compared with group A, B and C, group D had poor cognitive ability (P<0.05). The number of surviving cells in the hippocampus CA1 region was significantly improved compared with the D group (P<0.05). Compared with the A group, the viable cells in the hippocampal CA1 region of the three experimental groups were significantly lower (P<0.05).ConclusionsTianjiang Niuhuang Powder can reduce seizures, improve cognition and effectively alleviate neuronal damage in hippocampal CA1 neurons in rats with epilepsy.