west china medical publishers
Author
  • Title
  • Author
  • Keyword
  • Abstract
Advance search
Advance search

Search

find Author "吴强" 38 results
  • 视紫红质类及卷曲蛋白受体在糖尿病视网膜病变发生发展中的作用

    鸟苷酸结合蛋白偶联受体(GPCRs)是一类膜受体超家族, 被视为最好的药物靶点。在糖尿病视网膜病变(DR)进程中有大量不同亚型GPCRs参与。其中, 视紫红质类和卷曲蛋白(Frizzled)受体广受关注, 研究方向主要为视网膜炎症反应、新生血管生成、神经元和神经胶质细胞损伤等。血管紧张素Ⅱ受体是最为熟知的视紫红质类受体亚家族。应用血管紧张素Ⅱ受体1拮抗剂可显著降低1型糖尿病患者发生DR的可能性, 但无法减缓已并发DR患者的病变进展; 可减缓并发轻中度DR的2型糖尿病患者的病变进展。其他的视紫红质类受体还有趋化因子受体、大麻素相关受体、GPR91、GPR109A、APJ受体等。Frizzled受体是Wnt信号通路重要的膜受体等。在DR动物模型中, 使用Wnt通路阻断剂Dickkopf homolog 1能改善视网膜炎症、血管渗出、新生血管生成等。但Wnt通路参与DR进展的具体机制有待研究。随着对GPCRs与DR关系了解的加深, 未来将有更多以GPCRs为治疗靶点的药物应用于临床, 为DR患者带来福音。

    Release date: Export PDF Favorites Scan
  • Müller细胞生理功能及其在糖尿病视网膜病变中的变化

    Müller细胞接触并包裹视网膜神经元细胞体和突触, 对视网膜神经元的功能及代谢起到支持作用; 对维护视网膜细胞外环境的稳定, 如离子、水平衡和血视网膜屏障(BRB)等具有重要调控作用; 可释放神经胶质递质和刺激性神经物质, 通过对神经递质的再吸收循环, 为视网膜神经元提供神经递质前体进而影响神经突触的活性。此外, Müller细胞对病理刺激能够产生反应。该反应一方面具有视网膜神经元保护作用, 如分泌神经营养因子、吸收降解兴奋性毒素、分泌抗氧化剂等, 另一方面也可引起视网膜神经元谷氨酸盐代谢紊乱和离子平衡紊乱, 导致视网膜水肿和神经元变性损伤。Müller细胞对糖尿病视网膜病变(DR)的发生发展具有重要影响。DR可引起Müller细胞增生, 除造成谷氨酸盐代谢紊乱外, 还会引起Müller细胞大量分泌炎症介质和血管内皮生长因子等破坏BRB。深入研究Müller细胞, 对探讨DR的发病及防治具有重要意义。针对Müller细胞靶向转染的腺病毒载体研制成功, 利用两亲肽携带蛋白或抗体直接转染细胞达到抑制DR的效果, 这些方法为早期防治DR提供了新的途径。

    Release date: Export PDF Favorites Scan
  • 半阻断升主动脉心内直视手术92例

    Release date:2016-08-30 06:32 Export PDF Favorites Scan
  • 左心室部分切除减容术治疗终末期扩张型心肌病

    Release date:2016-08-30 06:33 Export PDF Favorites Scan
  • The effects and mechanisms of G protein-coupled receptor 91 on blood-retinal barrier in diabetic rats

    ObjectiveTo investigate the effects and mechanisms of G protein-coupled receptor 91 (GPR91) on blood-retinal barrier (BRB) in diabetic rats. MethodsA lentiviral vector of shRNA targeting rat GPR91 and scrambled shRNA were constructed. Healthy male Sprague-Dawley (SD) rats were selected in this study. The 60 rats were randomized into 4 groups and treated as follows:(1) control group (Group A, n=15), the rats received injections of an equal volume of 0.1% citrate buffer; (2) streptozocin (STZ) group (Group B, n=15), the rats received injections of STZ; (3) LV.shScrambled group (Group C, n=15), diabetic rats received an intravitreal injection of 1 μl 1×108 TU/ml scrambled shRNA lentiviral particles at 2 weeks after the induction of diabetes; (4) LV.shGPR91 group (Group D, n=15), diabetic rats received an intravitreal injection of 1 μl 1×108 TU/ml pGCSIL-GFP-shGPR91 lentiviral particles. At 12 weeks after intravitreal injection, immunohistochemistry and Western blot were used to assess the expression of GPR91, p-extracellular signal-regulated kinase(ERK)1/2, t-ERK1/2, p-Jun N-terminal kinase (JNK), t-JNK, p-p38 mitogen-activated protein kinase (MAPK) and t-p38 MAPK. Haematoxylin and eosin (HE) staining and Evans blue dye were used to assess the structure and function of the retinal vessel. Immunohistochemistry enzyme-linked immunosorbent assay (ELISA) was used to test the protein level of VEGF. ResultsImmunohistochemistry staining showed that GPR91 was predominantly localized to the cell bodies of the ganglion cell layer. Western blot showed that GPR91 expression in Group D decreased significantly compared with Group C (F=39.31, P < 0.01). HE staining showed that the retina tissue in Group B and C developed telangiectatic vessels in the inner layer of retina, while the telangiectatic vessels attenuated in Group D. It was also demonstrated in Evans blue dye that the microvascular leakage in Group D decreased by (33.8±4.11)% compared with Group C and there was significant difference (F=30.35, P < 0.05). The results of ELISA showed the VEGF secretion of Group B and C increased compared with Group A and the VEGF expression in Group D was significantly down regulated after silencing GPR91 gene (F=253.15, P < 0.05).The results of Western blot indicated that compared with Group A, the expressions of p-ERK1/2, p-JNK and p-p38 MAPK were significantly upregulated (q=6.38, 2.94, 3.45;P < 0.05). Meanwhile, the activation of ERK1/2 was inhibited by GPR91 shRNA and the difference was statistically significant (F=22.50, P < 0.05). ConclusionsThe intravitreal injection of GPR91 shRNA attenuated the leakage of BRB in diabetic rats. GPR91 regulated the VEGF release and the leakage of BRB possibly through the ERK1/2 signaling pathway.

    Release date: Export PDF Favorites Scan
  • Research progress and clinical application of retinal vascular diameter measurement

    Retinal blood vessels are the only circulatory system that can be observed under non-invasive conditions. By observing the morphological changes of retinal blood vessels, the changes of blood circulation can be indirectly reflected. The occurrence, development and evolution of different diseases can be discovered. With the development of new detection technologies, especially the wide application of fundus photography and optical coherence tomography, a more intuitive and non-invasive quantitative index is provided for retinal vascular measurement. It is important for the diagnosis, guiding treatment and follow-up of related vascular diseases. This article reviews the development of retinal vessel diameter measurement methods and related applications in clinical diagnosis and treatment.

    Release date:2020-12-18 07:08 Export PDF Favorites Scan
  • Investigation on Surgical Treatment of Abdominal Incisional Hernia(Analysis for 78 Cases)

    Objective To review the clinical operation methods of abdominal incisional hernia. Methods Classification, operation method and fellow-up of 78 patients with abdominal incisional hernia were retrospectively analyzed. Results The average time of fellow-up was 26 months. Nineteen cases were repaired with simple suture with 3 cases (15.8%) recurrence, 57 cases were repaired with man-made material with 2 case (3.4%) recurrence. Conclusions Individual operation method should be chosen according to body condition, classification of the size of abdominal loss and abdominal hypertension. It is an effective method to repair the hernia of abdominal incision with man-made material.

    Release date:2016-09-08 11:04 Export PDF Favorites Scan
  • Clinical Comparative Study of Tension-Free Herniorrhaphy with Different Suture

    目的 观察运用两种不同缝线固定修补材料对疝修补术后的复发、切口感染、慢性疼痛等并发症发生情况。方法 对2008年4月至2010年4月期间笔者所在科室收治的250例腹股沟疝患者行无张力疝修补手术时,采用多股丝线或可吸收合成缝线固定修补材料进行前瞻性对比研究。结果 2组患者术后疝复发、切口感染和切口疼痛(包括慢性疼痛)发生率间的差异均无统计学意义(P>0.05)。结论 腹股沟疝无张力修补术后的复发、切口感染、慢性疼痛等并发症的发生与缝线选择无关。术者的操作技巧、严格的无菌操作原则、彻底止血以及组织损伤小才是防止术后感染、慢性疼痛等并发症发生的重要因素。

    Release date: Export PDF Favorites Scan
  • 矫形鞋垫对足底筋膜炎患者的近期疗效

    目的 研究矫形鞋垫对治疗足底筋膜炎的疗效,并与常规的矫形器干预治疗(夜间背屈固定治疗)和运动疗法(跟腱和足底筋膜牵拉治疗)进行比较探讨其疗效。 方法 将2011年2月-11月收治的足底筋膜炎患者120例按随机数字表法分为矫形鞋垫治疗组(42例)和夜间踝背屈固定治疗组(37例)以及跟腱、足底筋膜牵拉治疗组(41例)。3组患者均于治疗前、治疗2周后和治疗8周后采用视觉模拟评级法以及功能评价评定双足负重时疼痛的强度、患者1周内最长可持续行走时间。 结果 患者治疗2周后及治疗后8周随访发现,3个治疗组的视觉模拟评分和患者最长可持续行走时间较治疗前均有明显提高,组间比较发现,穿戴矫形鞋垫治疗足底筋膜炎疗效优于夜间背屈固定治疗组和跟腱、足底筋膜牵拉治疗组。 结论 穿戴矫形鞋垫治疗足底筋膜炎的近期效果明显,比夜间背屈固定和牵拉跟腱及足底筋膜治疗足底筋膜炎疗效更优。

    Release date:2016-09-07 02:34 Export PDF Favorites Scan
  • Relationship between glomerular filtration rate and diabetic retinopathy in type 2 diabetic patients

    Objective To investigate the relationship between glomerular filtration rate (GFR) and diabetic retinopathy (DR) and macular thickness in patients with type 2 diabetes mellitus (T2DM). Methods A total of 161 T2DM inpatients were enrolled in this study. There were 95 males (95 eyes) and 66 females (66 eyes), with an average age of (62.2±11.0) years. The average duration of diabetes was (14.8±7.9) years. The patients were grouped according to the degree of DR. Among them, 91 patients were no DR, 24 patients were mild non-proliferative DR (NPDR), 24 patients were moderate NPDR, 13 patients were severe NPDR and 9 eyes were proliferative DR (PDR). Severe NPDR and PDR were combine into severe DR group for statistical analysis. All patients underwent direct ophthalmoscope, fundus colorized photography, spectral domain optical coherence tomography (SD-OCT), fasting blood-glucose, glycated hemoglobin and renal function examinations. GFR was evaluated by99 mTcDTPA. DR degree was evaluated by direct ophthalmoscope and fundus colorized photography. Central subfield (CSF), central macular volume and mean retinal thickness (MRT) were measure by SD-OCT. The correlation between GFR and DR staging and macular retinal thickness were analyzed by Spearman correlation analysis and Pearson correlation analysis. Logistic regression analysis was used to analyze the correlation between GFR and presence of DR. Results GFR was gradually decreased in patients with no DR, mild NPDR, moderate NPDR and severe DR (F=12.32,P<0.001). Pearson correlation analysis demonstrated that GFR was negatively correlated to CSF (r=−0.202,P=0.010); but no correlation with MRT (r=−0.087,P=0.272). Spearman correlation analysis demonstrated that GFR was negatively correlated to DR staging (r=−0.325,P<0.001). The difference of DR prevalence rate in normal, slight abnormal renal function and renal insufficiency patients was significant (χ2=12.32,P=0.002). Logistic regression analysis demonstrated that lower levels of GFR was significantly associated with presence of DR (95% confidence interval=1.71–4.32, odds ratio=2.72,P<0.001). Conclusion In T2DM patients, GFR is negatively correlated to DR staging and CSF. Lower GFR is independent risk factors for DR.

    Release date:2017-05-15 12:38 Export PDF Favorites Scan
4 pages Previous 1 2 3 4 Next

Format

Content