Objective To study the relationship between blood metastasis of colorectal cancer and cancer metastasis related factors.MethodsCK20 mRNA in peripheral blood was investigated by reverse transcription polymerase chain reaction (RTPCR) and proteins of CD44v6 and p53 in cancer tissues were examined by immunohistochemical in 50 cases of colorectal neoplasm. ResultsThe results showed that the positive rates of peripheral blood micrometastasis of colorectal cancer were 68%. It escalated along with the rising of the Dukes stage, the rates in Dukes C and D stage were significantly higher than that in Dukes A and B stage. The positive rates of CD44v6,p53 expression in colorectal cancer were 74% and 62% respectively. The positive rates of CD44v6 and p53 in Dukes A and B stage were significantly lower than those in Dukes C and D stage,in peripheral blood and colorectal cancer micrometastasispositive group were significantly higher than that in the micrometastasisnegative group. CK20 mRNA was significantly correlated with expressions of CD44v6 and p53 in cancer tissues. Conclusion The detection of CK20 mRNA in blood before operation and after operation examination of CD44v6 and p53 in cancer tissues are helpful for prediction of blood metastasis of colorectal neoplasm and postoperative treatment.
目的 探讨腹腔镜超低位直肠癌保肛手术的可行性。方法 回顾性总结2004年9月至2007年10月期间行腹腔镜超低位直肠癌保肛手术58例患者的临床资料。结果 54例在腹腔镜下顺利完成手术，中转开腹4例，无手术死亡病例。平均手术时间187 min，术中平均失血110 ml，术后肛门排气时间平均为2.3 d。手术切除淋巴结平均为18.5个； 随访6~42个月(平均17.6个月)未见切口种植及吻合口复发。结论 腹腔镜超低位直肠癌保肛手术具有创伤小、术后恢复快等优点，可以取得比开腹手术更好的根治效果。
目的 探讨腹腔镜下治疗粘连性肠梗阻的可行性和效果。 方法 分析在腹腔镜下对粘连性肠梗阻行粘连松解术的33例病例。结果 33例均顺利完成腹腔镜下粘连松解术，术后恢复好，无手术并发症发生； 随防6～15个月，均无腹痛、腹胀等症状复发。结论 利用腹腔镜对粘连性肠梗阻有选择地进行粘连松解手术安全、可行，能提高粘连性肠梗阻的治疗效果。