Using 70 SD white rats, diveded in two groups at random, after the common carotid artery wa(?) exposed, anastomosis of the artery was done by whole-layer suture and suture without including the endothelial layer, respectively. The rate of patency of both groups immediately after operation was 100 percent, where as in late stage, 94 percent and 97 percent, respectively. From the histologic exam ination, it was found that in the group of whole-layer suture, the time required to cover the sutureline with endothelium was delayed and there was rupture of the clastic fibers.
Abstract An animal experiment had been conducted for the purpose to find out the possibility of application of instantaneous sustained limited expansion (ISLE). A total of 54 skin defects in 9 pigs were obtained and were divided into two groups. One group of the pigs were subjected to instantaneous sustained limited expansion, the others, as the control group, just received the tension suture. At the same time the blood supply of the skin was measured by laser doppler flowmeter (LDF). It was showed that microcirculatory perfusion of the ISLE group was considerably better than that of the control. Inaddition, the correlation between the blood flow of the skin and the expanding pressure, as well as the pathologic changes of the skin were discussed.
In this experiment ,the sciatic nerves or twen-ty-eight rats were cut and then anastomosed withbiological adhesive agent or by suture in randonand the latter as control. The biological adhesive a-gent from human plasma was composed of fibrino-gen coagulase and medullary sheath of the nervetaken at the anasomosis region were studied histol-gically. The results of the experimental group wsasignificantly superior to the control.
Motor endplate is the structure connected between the nerve terminal and muscle fibre and plays a very important role in conducting nerve impulses to the target, therefore, systemical study of the sequential changes of the motor end-plate upon denervation is quite important.Ninety New Zealand rabbits were divided into nine groups from two weeks to nine months after denervation. Acetycholinesterase(AchE) was analyzed quantitatively to study the sequential changes of the motorendplates of tibialis anterior muscle. The results showed that there was no significant reduction of AchE at theend if one month after denervation, whereas a sharp reduction of AchE afterwards. AchE could not be stained after five month denervation.
In this experiment, two proximal ends of themedian and ulnar nerves of rabbit wereapproxirnated within the chitin tube for thepurpose to inhibit the neuroma formation. Byobservation under light and transmission electronmicrnscopo and immunohistochemistry, wefound that: (1) the axons of the two proximalstumpe could regenerate in the chitin tube for 2to 5mm, and then ceased to grow when anaxonal overlap happened resulting in inhibitingneuroma formation; (2) chitin tube could bedegradated a...
The aim of this experiment was to study the osteogenesis in vivo of allogenic osteoblast combined culture with calcium phosphate composites. The osteoblasts were obtained by enzymatic digestion of periosteum from fibula subcultured to 13 generations, the cells were combined culture with hydroxyapatite and biphasic calcium phosphate. Subseguently, the composite was implanted into rabbits subcutaneously or intramuscularly. The blank material was implanted in the contralateral side as control. Four weeks later, all animals were sacrificed. All the implants were examined by gross observation, histological examination and EDXA. The results showed: 1. obvious ingrowth of connective tissue with very little inflammatory reaction; 2. new bone formation in the composites with deposit of Ca and P on the surface of osteoblast, but none in the blank materials; 3. no significant difference of new bone formation between the different sites of implantation or different materials, but those implanted intramuscularly had lamellae form of new bone while those implanted subcutaneously had only mineralization of extracellular matrix. The conclusion were: 1. the composites are biocompatible with prior osteogenesis property; 2. periosteal-derived allogenic osteoblasts obatined by enzymatic digestion could survive following implantation with bioactivity; 3. rich blood supply might be advantageous to new bone formation and its maturation.
This experiment was to study the blood supply of diffcrent types of skin flaps and the revascularization of the host region. The types of skin flaps used in this cxpcriment were the axial pattern flap and the random pattern flap on the back of rabbite. Forty New Zealand rabbits were divided into 5 groups at random. In gathering the data For assessment, besides the local changes such as color, swelling of the flaps, the area of survival of flaps after division of the pedicles, the tests used to observe the postoperative changes included the isotope (99mTc) clearance test, intravenous orescein test, tissue transparent method by perfusion of the flap vessels with Chengdu ink and histologie study. The following conclusions cule be drawn: The complete revaseularization of random pattern flap occurred at 10 days after operation and that of xaial pattern flap was 14 days, the random pattern flaps had a quicker rate of revascularization. It was suggested that the flap ischemia was a factor which enhanced revascularization.
To study the blood supply and revascularization of super thin skin flaps with a presserved subcutaneous vascular retwork , we carried out a randomized self-matched experimental study in 10pigs. The free skin grafts with a preserved subcutaneous vascular network served as control. All skingrafts and flaps were divided into 6 groupe, besed on the potoperative days (1st , 3rd, 5th , 7th , 10thand 14th day). The revascularization of flape were assessed by means of intravenous fluorescein test...
Abstract In order to find a new method to repair large bone defect, the free periosteum autograft was investigated in experiment, and then the method was used clinically. In the experiment, a 6mm×18mm×5mm bone defect was made at upper end of both tibiae of 42 rabbites. The periosteum of each rabbit was cut into 1mm cubes, and implanted randomly into the tbial bone defect on one side and the other side was used as control. After 2, 4, 8 weeks, the bone defects of each group were examined for bone formation by roentgenography, radionuclide and histology. The results showed that the defects treated by free periosteum autografts healed twice as fast as the controls (its natural healing). The reason probably was that the periosteum provided with many osteogenic cells. On thebasis of these results, 21 cases of bone defects (the largest was 10.5cm×4cm×4cm, the smallest was 2cm×2cm×2cm) including 17 cases of benign bone tumor and4 cases of chronic osteomyelitis, were treated by free periosteum autografts. The defects were all healed, and the function of the joints was restored.