ObjectiveTo summarize the progress of p120-catenin (p120ctn)——a new member of catenin family in tumor research. MethodsDemestic and international published literatures related to p120ctn in recent years were collected and reviewed. Results① p120ctn was involved in formation of cadherincatenin complex, participated in cell growth, proliferation, and adheren junctions. ② p120ctn regulated Rho GTP activity and promoted cell motility. ③ p120ctn was involved in the regulation of gene transcription through binding with the nuclear transcription factor Kaiso. ④ p120ctn was involved in angiogenesis process induced by vascular endothelial growth factor. ⑤ p120ctn was involved in inflammation, cell malignant transformation, and tumor invasion and metastasis. ConclusionsAs a new member of catenin family, p120ctn participates in a variety of biological processes relying on its cellular localization. It will be facilitated to judge the genesis and progression of tumor from the abnormal alteration of p120ctn according to understanding the biological function and mechanism of p120ctn in the molecular level, a new pathway in the prevention and treatment of cancer is provided.
Objective To summarize the advancement on Lewis-Selectin metastasis pathway and anti-tumor treatment of cimetidine in colorectal cancer.Methods Domestic and international publications involving Lewis-Selectin metastasis pathway and antitumor role of cimetidine in recent years were collected and reviewed. Results The expression of sialyl Lewis X (sLeX) was most significant in Lewis-Selectin metastasis pathway in colorectal cancer. Cancer cells could interact with E-selectin and P-selectin of activated vascular endothelial cells by sLeX ligand on the cell surface, thereby prompting invasion and metastasis. Cimetidine could inhibit the growth of tumor, improve immune response of the host to tumor cell, inhibit angiogenesis of tumor and metastasis. The curative effect of cimetidine on colorectal cancer patients with positive sLeX expression was better than that with negative sLeX expression. Conclusion Lewis-Selectin metastasis pathway is one of the important metastasis pathway in colorectal cancer. Cimetidine can prevent metastasis of colorectal cancer by blocking sLeX expression.
ObjectiveTo study the relationship of the expression of CD44v6 and bcl2 protein with histological type,pathological grading and metastasis.MethodsImmunohistochemical technique was used to investigate the expression of CD44v6 and bcl2 in 50 primary gallbladder carcinoma,20 gallbladder adenoma and 10 chronic cholecystitis.ResultsThe positive rate of CD44v6 and bcl2 was 82.0% and 60.0%，which was positively correlated with the histological type,pathological grading and metastasis of gallbladder carcinoma(P＜0.05) and was higher than that in gallbladder adenoma (CD44v6 45.0% and bcl2 30.0% respectively).Expression of CD44v6 was significantly correlated with the expression of bcl2(r=0.36,P＜0.05).ConclusionCD44v6 and bcl2 might be an important biologic marker to evaluate the malignancy and prognosis of gallbladder carcinoma.There might be some extent of coordinated regulation between them.
目的降低远端胰腺切除术后胰漏的发生率。方法对34例患者施行远端胰腺切除时，近端稍外凸，呈“gt;”形，然后结扎主胰管，断面缝扎止血，联合应用带血管蒂胃浆肌瓣或空肠浆肌瓣与纤维蛋白胶粘合缝闭胰腺残端。结果34例患者均未发生胰漏、腹腔感染、脓肿及胰腺假性囊肿。结论带血管蒂胃、空肠浆肌瓣有利于促进胰断面愈合，联合应用纤维蛋白胶能有效地预防胰漏。
【Abstract】Objective To study the relationship of the expression of CD44v6 mRNA and nm23H1 mRNA with the clinical pathology parameter and prognosis of breast cancer, and to investigate the correlation of the expression of CD44v6 mRNA and nm23H1 mRNA. Methods In situ hybridization and CSA immunohistochemistry method were used to detect the expression of CD44v6 mRNA and nm23H1 mRNA in 94 cases of breast cancer. Results The positive expression of CD44v6 mRNA and the negative expression of nm23H1 mRNA were positively correlated with the grading, clinical stage, lymph node metastasis, and recurrence and prognosis of breast cancer. CD44v6 mRNA expression and nm23H1 mRNA were negatively correlated in breast cancer. Patients who had positive expression of CD44v6 mRNA and negative expression of nm23H1 mRNA had a higher lymph node metastatic rate and a lower survival rate. Conclusion Several genes were involved in the occurrence and development of breast cancer in which the expression of CD44v6 mRNA has synergistic action in negative regulation with that of nm23H1 mRNA. Combined detection of the expression of these two mRNA is helpful to judge the metastasis, recurrence and prognosis of breast cancer.
Objective To investigate the level of cell adhesion molecule sialyl-LeX expression in colorectal carcinoma and its relation with carcinogenesis, differentiation, metastasis and prognosis. Methods Sialyl -LeX expression and its optical density in colorectal carcinoma (n=90) and remote normal mucosa (n=30) were quantitatively studied with microwave-LSAB immunohistochemical method combined with image analysis technique. Fifty-three patients were followed up. Results The weaker staining in remote normal colorectal mucosa was observed in very limited parts of some deep crypts. Positive rate of sialyl-LeX expression was only 16.7%(5/30). The positive expression of sialyl-LeX was observed in 83 of 90 patients with colorectal carcinoma(92.2%). The apical cytoplasma of cancer tubules, the luminal contents, and the cytoplasma of the cancer cells were bly stained. The mean integral optical density of sialyl-LeX positive cell in poorly differentiated adenocarcinoma was significantly higher than that in highly differentiated and mucinous ones (Plt;0.01). It was markedly higher in patients with positive lymphatic nodes than that in negative ones (Plt;0.01). With followed-up for longer than 5 years, it was much lower in the alive cases than that in the dead (Plt;0.01). Conclusion These findings indicate that changes of sialyl-LeX expression and its optical density is related to carcinogenesis, differentiation, invasion and metastasis of colorectal carcinoma. It may be a good predicter for the prognosis of patients with colorectal carcinoma.
【Abstract】Objective To study the advances in clinical application of shape memory alloy (SMA) in general surgery. Methods The literature in the recent years on the advances of SMA was reviewed. Results At present clinical application of SMA have been developed. Different stents could dilate the benign and malignant stricture of blood vessels, biliary ducts, cola, recta and gastric outlet obstruction. The SMA catheter system was effective for peroral cholangiopancreatoscopy. Novel nitinol basket instrument might be useful for percutaneous cholecystolithotomy. Compression anastomoses was safe and sound in gastrointestinal surgery using a device made of SMA. Nitinol stent could be used in transjugular intrahepatic portosystemic stent shunt (TIPSS) with satisfactory results. Nitinol occlusion devices was superior to stainless steel coils. The Simon nitinol filter represented a new generation of venous interruption devices designed to prevent recurrent pulmonary embolism. Conclusion Clinical application of SMA will be increased by the development of endoscope, laparoscope and interventional technique.
To study the relationship between the expression and contents of cell adhesion molecule CD15 and differentiation and lymph nodes metastasis of breast carcinomas, CD15 expression and its contents in 94 cases of breast carcinomas and or cases of normal breast tissue were evaluated by microwave-SP immunohistochemical chenique combined with image analysis. CD15 immunoreactivity in normal breast tissue was mainly localised at the border of gland, but in breast cancer tissues it was mainly localised in the membrane and cytoplasm. Positive rate of CD15 and its average optic density in breast carcinomas were significantly higher than those in normal breast tissue (Ｐ＜0.05 and Ｐ＜0.001, respectively). The worse tumors differentiated and the earlier lymph nodes metastasized, the higher CD15 expressed and its optic density was measured (Ｐ＜0.05 and Ｐ＜0.001, respectively). These results suggest that CD15 expression and its contents might be a useful indicator to evaluate the malignancy and biological features, and could be considered as a good prognostic predictor for breast carcinomas.
目的 观察内给氧治疗重症急性胰腺炎的临床效果。方法 对2005年2月至2009年3月期间收治的55例重症急性胰腺炎患者采用非手术治疗，在给予奥曲肽或施他宁、抗生素及基础性支持治疗的基础上，按入院时间先后分为2组，试验组(n=28)给予注射用内给氧治疗1周，对照组(n=27)未用内给氧。结果 试验组动脉血氧分压在应用内给氧治疗前为(53.8±8.1) mm Hg，治疗后为(87.9±9.5) mm Hg； 血氧饱和度治疗前为（81.1±7.4）%，治疗后为（93.2±6.7）%； 治愈病例中血淀粉酶降至正常的时间，试验组平均为(29.4±7.2) h，对照组平均为(162.1±46.8) h； 死于急性呼吸窘迫综合征者试验组为0（0/28），对照组为14.8%（4/27）; 上述指标差异均有统计学意义（P＜0.05）。结论 内给氧配合基础性治疗对重症急性胰腺炎有较好疗效，实用性较强。