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find Keyword "建立" 6 results
  • Establishment of AdriamycinResistance Cell Substrain SMMC7721/Adriamycin of Human Hepatocellular Carcinoma and Research of Its Characteristics

    ObjectiveTo establish multidrugresistance cell substrain of human hepatocellular carcinoma and to investigate its characteristics.MethodsSMMC7721 cell strain was cultured in Adriamycin(ADM). The multidrugresistance cell substrain SMMC7721/ADM was harvested after a long period of culture by gradually increasing the concentration of ADM and its characteristics were investigated. Results①The drug resistance of SMMC7721/ADM to ADM increased by 33.3 times, to Vincristine 16.8 times, to Diamminedichloroplatinum 2.8 times. ②The drug resistance cell substrain had almost the same growth velocity as its parental generation. The doubling time was 32.0 hours and 30.5 hours respectively. They had the analogous growth curves. ③The obvious difference between the drug resistance cell substrain and its parental generation was that the former’s microvilli became thick, short and scattered by scanning and transmitting electron microscopy. ④The multidrug resistance cell substrain kept the characteristics of hepatocellular carcinoma, it could be transplanted into the subcutaneous tissue of nude mice. ⑤The drug resistance of the cell substrain reduced to 28.0% and 9.2%after removal of the drug for 1 month and 2 months respectively, its drug resistance could remain stable (35.4 times) after 2 months of culture in ADM (0.04 μg/ml).ConclusionThe SMMC7721/ADM cell substrain has the stable fundamental characteristics of a drug resistance cell strain.

    Release date:2016-08-28 04:43 Export PDF Favorites Scan
  • ESTABLISHMENT AND EVALUATION OF HYDROCEPHALUS MODEL IN DOGS

    Objective To establish and evaluate a hydrocephalus model in dogs. Methods Twelve healthy adult male mongrel dogs (weight, 10-15 kg) were randomly divided into the control group (n=6) and the experimental group (n=6). All the dogs were given CT and neurological examination to exclude congenital ventricular enlargement and neurological abnormity before they received hydrocephalus induction. Surgical procedures included the exposing of the foramen magnum area, the opening of the atlantooccipita anadesma, and the injecting of silicone oil (0.3 ml/kg) into the fourth ventricle through a silicone tube. Normal saline was injected in the control group. The Tarlov neurological fitness assessment and the Evan’s ratio were used to evaluatethe degree of hydrocephalus at 3, 14 and 56 days after operation. Results In the experimental group, the dogs were dull and unsteady in walking,and they drank and ate less. The lateral ventricle began to expand 3 days afteroperation, and then the temple horn of the lateral ventricle and the third ventricle were also affected 14 days after operation. The ventricles were enlarged progressively after operation. The Tarlov scores measured at 3, 14 and 56 days afteroperation had a significant difference at the same time point between the control group(5.83±0.75,6.50±0.55,6.00±0.63) and the experimental group (4.00±0.89,4.83±1.17,4.50±1.05,P<0.01), but had no significant difference within the same group at different time points (P>0.05). The Evan’s ratios measured at 3, 14 and 56 days after operation were 0.33±0.04,0.39±006,0.44±0.03,respectively,in the experimental group; and were 0.27±0.06,0.25±0.09, 0.26±0.05,respectively,in the control group. There was a significant difference atthe same time point between the two groups, and at different time points within the experimental group (P<0.05).Conclusion The dog model of hydrocephalus induced by the injecting of silicone oil into the fourth ventricle has a highsuccess rate, and the model is appropriate for the studies on diagnosis and therapy of hydrocephalus. 

    Release date:2016-09-01 09:23 Export PDF Favorites Scan
  • 药剂科在突发事件中应急机制的建立及应用

    【摘要】 近年来全球各类重大突发事件屡屡发生,对人类生命安全和社会经济发展构成了极大威胁,成为全世界关注的焦点,如2003 年中国等国家和地区发生SARS及四川5•12汶川大地震等。建立应对突发事件的应急机制,制定完备的应急预案、应急体系,成为各行业面临的新挑战。医疗机构关系人民生命健康,在各类突发事件中具有极其重要的作用,而完善的药学应急管理体系的建立,对于保障整个突发事件医疗工作的正常运行是不可或缺的重要一环。药剂科探讨建立的应对突发事件应急机制预案和完善应急体系,成为业内人员共同关注的新课题。

    Release date:2016-09-08 09:24 Export PDF Favorites Scan
  • 无痛病房的建立及管理进展

    【摘要】 通过对无痛病房概念、模式、管理及疼痛护理方式的全面阐述,指出无痛病房的建立,需要以医护人员的共同参与和多学科的合作为基础,需要有一套完善科学的管理制度、操作流程及评价体系为支撑,规范有效的疼痛护理方法与机制,是患者获得疼痛治疗与护理的重要保证。

    Release date:2016-09-08 09:26 Export PDF Favorites Scan
  • 两种腹膜后腔建立方法的比较

    【摘要】 目的 比较气囊法组和直接法组建立腹膜后腔的临床价值。 方法 对2005年5月-2008年12月32例后腹腔镜手术建立腹膜后腔的临床资料进行比较,统计分析两种腹膜后腔建立方法在时间、空间大小、食指经穿刺孔能否触及肾脏、并发症发生率、出血量等的差别。 结果 两种方法均成功建立腹膜后腔。气囊法组建立腹膜后腔时间平均为(13.17±1.40) min,直接法组为(4.45±1.20) min。气囊法组腹膜后腔空间为(396.00±13.33) mL,直接法组为(85.50±6.05) mL。气囊法组食指经穿刺孔能触及肾脏6例(50%),直接法组无1例(0%)。组间比较,差异有统计学意义(Plt;0.01)。两组患者建立空间后经观察均为少量渗血,气囊法组发生气囊爆裂1例。 结论 直接法组在时间上明显优于气囊法组,与气囊法组比较并未增加相关并发症,但建立的空间较小;在熟练掌握了气囊法组后可以运用直接法组建立腹膜后腔。

    Release date:2016-09-08 09:50 Export PDF Favorites Scan
  • Validation of multivariate selection method in clinical prediction models: based on MIMIC database

    ObjectiveTo verify the influence of different variable selection methods on the performance of clinical prediction models. MethodsThree sample sets were extracted from the MIMIC database (acute myocardial infarction group, sepsis group, and cerebral hemorrhage group) using the direct entry of COX regression, step by step forward, step by step backward, LASSO, and ridge regression, based on random forest. These existing six methods of variable importance algorithm, and the optimal variable set of different selected methods were used to construct the model. Through the C index, the area under the ROC curve (AUC value) and the calibration curve, and the results within and between groups were compared. ResultsThe variables and numbers selected by the six variable selection methods were different, however, whether it was within or between groups did not reflect which method had the advantage of significantly improving the performance of the model. ConclusionsPrior to using the variable selection method to establish a clinical prediction model, we should first clarify the research purpose and determine the type of data. Combining medical knowledge to select a method that can meet the data type and simultaneously achieve the research purpose.

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