【Abstract】ObjectiveTo detect the expression of human papilloma virus(HPV) 16 E7 was detected in colorectal adenocarcinoma tissue and normal mucosa. MethodsEighty-two patients with primary colorectal adenocarcinoma were selected in this study. The samples were taken from the tumor and the adjacent normal mucosa (10 cm away from the tumor) in each patient. Polymerase chain reaction (PCR) and immunohistochemistry were used to detect HPV16 E7 DNA and protein respectively. ResultsHPV16 E7 DNA expression was significantly higher in colorectal carcinoma (51.22%,42/82) than that in adjacent normal mucosa (4.88%,4/82), P＜0.01. A correlation was found between HPV16 E7 DNA expression and tumor location (P＜0.05)，18.18% in the ascending colon carcinoma and 64.10% in the rectal carcinoma. HPV16 E7 DNA expression was also associated with Dukes stage（P＜0.01）, but was not correlated with cancer differentiation. HPV16 E7 protein expression was mainly dectected in the nuclei of tumor cells with immunohistochemistry. There was a correlation between the expression of HPV16 E7 protein and HPV16 E7 gene. PCR had a higher sensitivity than immunohistochemistry. ConclusionHPV16 infection rate is much higher in the colorectal carcinoma than that in the adjacent normal mucosa, which indicates that HPV16 infection exists in some colorectal carcinomas. The high infection rate of HPV16 E7 is associated with advanced Dukes stage and proximity to anus.
Objective To discuss the mode of triage patient in Emergency Department of West China Hospital of Sichuan University after the Wenchuan earthquake and the major experience during the process. Method The patients’ severity level of injury and waiting time for medical care in Emergency Department were analyzed after earthquake in 2 weeks. Result 375 patients were triaged into the severity level and get the treatment followed the triage systems gave the highest rate of survival and lowest rate of over triage and under triage. Conclusion In the calamity of Wenchuan earthquake with large number of casualties, Emergency Department automatically shifted into disaster mode and triage victims by the irregular triage systems. In this situation, medical staff do not just triage the patients to the Emergency Department or to see a doctor, but triage the victims to the medical care immediately and efficiently. By the new triage system, the West China Hospital saves more lives in less time.
【Abstract】ObjectiveTo study Toll like receptor 4 (TLR4) expression on peripheral blood monocytes (PBMCs) during the early stage of human acute pancreatitis. MethodsThirty consecutive patients with acute pancreatitis admitted within 24 h of onset of abdominal pain were enrolled in this study. Another 20 healthy volunteers were included as control. Blood samples were collected by venipuncture on the day of admission and 3， 7 d after admission and PBMCs were isolated. TLR4 and CD14 expressions on PBMCs were detected by flow cytometry. Serum tumor necrosis factor alpha (TNF-α) and interleukin 6 (IL-6) were measured simultaneously. Correlations between these parameters were analyzed. ResultsTLR4 expression increased on the day of admission and then continued to decline for several days. On third day, TLR4 expression was almost normal compared with the normal control. The alteration of serum TNF-α was consistent with that of TLR4. ConclusionDuring the early stage of human acute pancreatitis, mononuclear-macrophages may be ignited through TLR4 (a door keeper of innate immune system), which lead to TNF-α production.
ObjectivesTo investigate the ability of emergency medical rescue personnel in Sichuan province to collect information and contact resources at rescue sites, and to provide evidence for emergency training and drills. MethodsThe rescue site of a batch of critically ill patients in public emergency was simulated. The cross-sectional survey was made on rescue personnel at the city (prefecture) and county level of Sichuan province. The rating scale of on-site information contact ability was used to evaluate their performance. Because the score distribution does not conform to the normal distribution, the space between the median and interquartile was used to describe the score, and multiple measurement data was compared by the rank sum test. ResultsA total of 287 rescue drill personnel were included. The overall score M (P25, P75) of information contact ability was 19.57 (13.04, 28.26). The scores of each dimension were as follows: the safety zone was set as 0 (0, 10), the on-site hazard identification was 0 (0, 16.67), external contact and coordination was 50 (0, 50), internal coordination and command was 50 (16.67, 50), the on-site disaster statistics was 40 (10, 70), the on-site resource status was 0 (0, 0), and the on-site reinforcement demand was 0 (0, 0). Hierarchy by occupation: 19.57 (12.50, 28.26) for clinicians, 19.57 (14.13, 34.78) for nurses, 25 (14.67, 32.61) for medical skills, 21.74 (14.13, 30.44) for public health doctors, and 17.39 (9.78, 21.74) for health management. Hierarchy by titles: 21.74 (13.04, 28.26) for intermediate level, and 17.39 (10.33, 23.91) for advanced level. Scores of different dimensions, occupations and titles were compared respectively, and the differences were statistically significant (P<0.05). ConclusionsThe results of this survey show that the emergency medical rescue personnel at the city (prefecture) and county level of Sichuan province have insufficient overall ability to contact information on site, so those with insufficient ability need to be trained. The training focuses on the on-site resource status, on-site reinforcement demands, safety zone setting and the ability to identify on-site hazards.