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find Author "张衍民" 2 results
  • Effects of Ambroxol on JNK Signaling Pathway in Gastric Aspiration Induced Lung Injury in Rats

    ObjectiveTo investigate the effect of ambroxol hydrochloride on c-Jun N-terminal kinase (JNK) signal pathway in gastric aspiration lung injury. MethodsForty healthy male Sprague Dawley rats were randomly divided into a control group, an injury group, a SP600125 (JNK specific inhibitor) group and an ambroxol group. The model of gastric aspiration lung injury was established by aspiration of gastric contents. The rats in the SP600125 group preoperatively received intravenous injection of JNK specific inhibitor SP600125 (3 mg/100 g). The rats in the ambroxol group received intravenous injection of ambroxol hydrochloride (50 mg/kg) 2 hours after the damage occurred. The neutrophil count and malondialdehyde (MDA) activity in bronchoalveolar lavage fluid (BALF), the lung wet weight/dry weight ratio (W/D), and myeloperoxidase (MPO) activity were measured. The protein expressions of JNK and phosphorylated JNK (p-JNK) and inducible nitric oxide synthase (iNOS) in lung tissue were detected by Western blot method. The changes of lung tissue structure were observed under light microscope. ResultsIn the injury group, the neutrophil counts and MDA activity in BALF, W/D, MPO activity, p-JNK and iNOS protein expression increased significantly, lung tissue appeared obvious histopathological injury compared with the control group. In the SP600125 group and the ambroxol group, neutrophil count and MDA activity in BALF, lung W/D, MPO activity, p-JNK and iNOS protein expression were significantly decreased compared with the injury group (P < 0.05), and the damage of the lung tissue pathology was reduced. The expression of JNK protein in lung tissue was not different in all groups (P > 0.05). ConclusionsJNK is involved in inflammatory reaction of gastric aspiration lung injury. The protective effect of ambroxol may be related to the inhibition of JNK signaling pathway and the inhibition of iNOS expression.

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  • 慢性阻塞性肺疾病急性加重引起患者疼痛的病因初步探讨

    目的初步探讨慢性阻塞性肺疾病(简称慢阻肺)急性加重引起患者不同部位疼痛发生的病因。方法回顾性分析自 2017 年 6 月至 2019 年 3 月入住徐州医科大学第二附属医院呼吸内科因慢阻肺急性加重引发不同部位疼痛的 50 例患者的临床资料。入选患者均依据病情需要给予化痰、抗感染、解痉止喘、吸氧改善通气等综合治疗。评估时间点为入院初治疗前、治疗后第 3 天、治疗后 1 周;评估方法采用简式麦吉尔疼痛问卷(SF-MPQ)量表对各时间点患者疼痛情况进行评分。同时评估各时间点患者血细胞分析、降钙素原、超敏 C 反应蛋白、血气分析;酶联免疫吸附试验法测定各时间点患者血清 5-羟色胺及缓激肽水平。结果与治疗前比较,经治疗后第 3 天、治疗后 1 周后,患者 SF-MPQ 疼痛量表评分值明显降低,血清 5-羟色胺及缓激肽水平降低(均 P<0.05)。患者 SF-MPQ 疼痛量表评分与血细胞分析中白细胞计数、超敏 C 反应蛋白、降钙素原、动脉血二氧化碳分压、血清 5-羟色胺及缓激肽水平呈正相关(均 P<0.05),与动脉血氧分压呈负相关(P<0.05)。结论慢阻肺急性加重引起的疼痛症状可能与全身炎症反应、低氧血症及体内二氧化碳潴留致高碳酸血症有一定关系。

    Release date:2021-01-26 05:01 Export PDF Favorites Scan
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