west china medical publishers
Keyword
  • Title
  • Author
  • Keyword
  • Abstract
Advance search
Advance search

Search

find Keyword "心脏停搏液" 11 results
  • Changes of the Level of G Protein in Newborn Guinea-pig Myocardium Undergoing Global Ischemic Reperfusion

    ObjectiveTo study the changes of levels of α subunits of stimulatory (Gsα) and inhibitory guanine nucleotide binding protein (Giα) in newborn guinea pig (0 2 days old) myocardium undergoing global ischemic reperfusion, and influences on the changes by St.Thomas Ⅱ and cold blood cardioplegic solution.MethodsThirty newborn guinea pigs were randomly assigned to three groups. GroupⅠ ( n = 10): the newborn hearts suffered by hypothermic global ischemia; group Ⅱ( n =10): the newborn hearts arrested by St. Thomas Ⅱ , and group Ⅲ ( n = 10): the newborn hearts arrested by cold blood cardioplegic solution. Levels of Gsα and Giα were investigated with Western blot analysis.ResultsNo differences of levels of Gsα and Giα were found in three groups before ischemia ( P gt;0.05). The level of Gsα after ischemia was significantly decreased than before ischemia in groupⅠand group Ⅱ ( P lt; 0 01), whereas no pronounced changes in group Ⅲ ( P gt;0.05) were noted after ischemia. The level of Gsα in group Ⅲ was not significantly changed after reperfusion compared with before ischemia( P gt;0 05), and it was much higher than those in groupⅠand group Ⅱ ( P lt; 0 01). Level of Giα was found not markedly changed in group Ⅲ after reperfusion compared with that before ischemia, but was notable higher in groupⅠand group Ⅱ( P lt;0.01). ConclusionsSignificant decrease of level of Gsα, whereas marked increase of level of Giα are found in myocardium of newborn guinea pig undergoing hypothermic (20℃) ischemic reperfusion. No impact of St. Thomas Ⅱ on these changes is verified, but recovery to the level of Gsα and Giα before ischemia is achieved by cold blood cardioplegic solution after ischemia and reperfusion. Unbalance between Gsα and Giα is the one of the mechanisms of ischemic reperfusion injury for immature myocardium.

    Release date:2016-08-30 06:24 Export PDF Favorites Scan
  • 腺苷含血心脏停搏液在心瓣膜置换术中对心肌的保护作用

    摘要: 目的 研究含血心脏停搏液中加入外源性腺苷在心瓣膜置换术中对心肌的保护作用。 方法 将32例行心瓣膜置换术患者随机分为两组,腺苷组在含血心脏停搏液中加入外源性腺苷,对照组单用含血心脏停搏液,分别经主动脉根部或切开主动脉经冠状动脉窦直接灌注。于术前、主动脉开放后6 h、24 h、72 h采集患者桡动脉血,监测心肌肌酸激酶同工酶(CKMB)、肌钙蛋白I(cTnI);观察心脏停搏情况,术后机械辅助通气时间及术后正性肌力药物的应用情况;透射电子显微镜观察心肌超微结构的改变。 结果 两组患者均无死亡。腺苷组诱导心脏停搏时间较对照组短(P=0.021);腺苷组的CK-MB水平在主动脉开放后6 h、24 h,cTnI水平在主动脉开放后6 h、24 h、72 h均较对照组低(Plt;0.05);两组机械辅助通气时间和术后多巴胺使用剂量差异无统计学意义(Pgt;0.05);腺苷组心肌超微结构心肌损伤较对照组明显减轻。 结论 外源性腺苷加入心脏停搏液中能显著提高对心肌的保护效果。

    Release date:2016-08-30 06:01 Export PDF Favorites Scan
  • 心肌肽在心脏手术中应用的安全性观察

    摘要: 目的 探讨注射用心肌肽在体外循环心脏手术中使用的安全性。 方法 选择2008年4月至10月在我院心脏外科择期行心脏手术患者100例,并分为两组,实验组:50例,男27例,女23例;年龄46.50±17.80岁;患者在麻醉后静脉滴注心肌肽1 mg/kg,30 min完成;在第1次的灌注液中一次性加入心肌肽2 mg/kg;于术后第1 d,2 d和3 d静脉滴注心肌肽3 mg/kg。对照组: 50例,男24例,女26例;年龄50.10±20.40岁;患者给予相同剂量的5%葡萄糖溶液。 术后5~7 d检测血、尿常规及生化检查,行心电图和彩色超声心动图等安全性指标检测。 结果 术后实验组血、尿常规及生化指标包括肝功能(门冬氨酸氨基转移酶29.10±18.19 U/L vs. 37.27±21.81 U/L)、肾功能(血清肌酐65.84±22.69 μmol/L vs. 68.50±17.71 μmol/L),血糖、血清电解质、心率、ST段、左心室舒张期末内径(48.00±7.59 mm vs. 50.23±5.76 mm)、左心室射血分数(69.00%±7.00% vs. 67.00%±9.00%)与对照组比较差异均无统计学意义(Pgt;0.05)。 结论 注射用心肌肽在心脏手术围手术期对人体重要器官无明显影响,使用是安全的。

    Release date:2016-08-30 06:02 Export PDF Favorites Scan
  • Protection Effect of Isolated Ischemic Heart by Polyethylene Glycolbovine Hemoglobin Solution

    Objective To investigate the effect of polyethylene glycolbovine hemoglobin (PEG-bHb), which was used as an oxygen carrier in cardioplegic solution, on the protection of isolated rat hearts. Methods The hearts of 32 male SD rats were harvested and transferred to Langendorff circuit. They were divided into 4 groups according to cardiocplegia: St.Thomas group (group A), 1∶2 PEG-bHb group (group B), 1∶4 PEG-bHb group (group C) and 1∶8 PEG-bHb group (group D). After 20min balance period, hearts were perfused with cold (4℃) cardioplegic solutions, and preserved at 30℃ for 60min, then reperfused. Levels of cardiac troponin I (cTn I) and adenosine triphosphate(ATP) contant in coronary effuent were detected, and ultrastructures of myocardium were observed. Results After reperfusion, cTn I contant of group A were higher (F=52.955,Plt;0.05) and ATP contant were lower (F=68.757,Plt;0.05) than those in group B, group C and group D. Myocardial water contant were lower in group B and group C(F=3.048,Plt;0.05). Conclusion PEG-bHb in cardioplegic solutions can provide better myocardial protection during ischemia.

    Release date:2016-08-30 06:09 Export PDF Favorites Scan
  • Protective Effects of Cardioplegic Solution with Exogenous Creatine Phosphate on Donor Heart Preservation in Rats

    Objective To investigate the protective effects of cardioplegic solution with creatine phosphate on donor heart preservation in rats, and the possibility of prolonging outofbody heart preservation in lack of blood supply to improve on transplantation quality. Methods Twenty Wistar rats were randomly divided into two groups, control group (n=10): pure St.ThomasⅡ cardioplegic solution was perfused to protect donor hearts; experimental group (n=10): St.ThomasⅡ cardioplegic solution with 2.5g/L sodium creatine phosphate was perfused to protect donor hearts. After 4 hours of refrigerated preservation, myocardial tissues were tested for adenosinetriphosphate(ATP) level and super oxide dismutase (SOD) activity. Cardiac ultrastructure and mitochondria swelling condition were monitored with light and electron microscopes. Results After the 4hour low temperature preservation of donor hearts, ATP content in experimental group cardiac tissue was significantly higher than that in control group (2.75±0.99μmol/mg vs. 1.77±0.86μmol/mg, Plt;0.05); SOD in experimental group cardiac tissue was significantly higher than that in control group(49.6±2.52 U/mg vs. 45.27±2.21 U/mg,Plt;0.05). Electron microscopic inspection demonstrates that in the control group, chromatin condensed and dissolved inside the nuclear membrane; mitochondria ridge gap disappeared; myocardial cell pyknosis and mesenchymal vascular endothelia necrosis appeared. In contrast, myocardial cell nuclei remained in the center; sarcomere band structure was distinct; sarcoplasmic reticulum expanded and intercalated disk band structure was clear in experimental group. Conclusion Cardioplegic solution with creatine phosphate is able to enhance donor heart myocardial protection significantly.

    Release date:2016-08-30 06:10 Export PDF Favorites Scan
  • 氧合冷血与St.Thomas心脏停搏液逆行灌注对心肌保护的比较

    目的对比氧合冷血与St.Thomas心脏停搏液持续逆行灌注对心肌保护的影响。方法选择2004年9月至2005年3月在我院行心瓣膜置换术患者74例,其中双瓣膜置换术11例,二尖瓣置换术(MVR)43例,主动脉瓣置换术(AVR)20例。74例被随机分为两组,A组:34例,St.Thomas停搏液持续逆行灌注;B组:40例,氧合冷血停搏液持续逆行灌注。于手术前、主动脉阻断即刻、主动脉开放即刻、术后2、6、12、24、48和72h测中心静脉血心肌肌钙蛋白I(cTnI)、肌酸激酶(CK)、肌酸激酶同工酶(CK—MB)、乳酸脱氢酶(LDH)。观察心脏复跳情况,并将两组间测量结果进行比较。结果术前两组cTnI、CK、CK—MB、LDH水平均在正常范围,开放主动脉后2h开始升高,48h后缓慢下降。两组间开放主动脉后2、6、12、48和72h,cTnI、CK、CK—MB、LDH测定结果比较差别有统计学意义(P〈0.05)。A组心脏自动复跳20例(58.82%),B组40例(100%),两组比较差别有统计学意义(P〈0.05)。结论与St.Thomas冷晶体心脏停搏液相比,氧合冷血心脏停搏液持续灌注具有更好的心肌保护作用。

    Release date:2016-08-30 06:22 Export PDF Favorites Scan
  • 新型心脏停搏液灌注器在心肌保护中的应用

    目的报告新型心脏停搏液灌注器在心肌保护中的应用。方法100例心脏病患者在心内直视术中采用自行设计制造的高钾温-冷-温血心脏停搏液灌注器和新的高钾氧合血配备方法进行心肌保护。结果体外循环时间31~163min,平均52.5min,主动脉阻断时间11~112min,平均42.6min,心脏自动复跳率100%,均为窦性心律。结论自行设计的新型心脏停搏液灌注器和新的高钾氧合血配备方法在心内直视手术中的心肌保护效果确切、满意,有一定的临床应用价值。

    Release date:2016-08-30 06:26 Export PDF Favorites Scan
  • 1,6-二磷酸果糖和巯甲丙脯酸对心脏术后心肌缺血-再灌注损伤的保护

    摘要 目的 探讨1,6-二磷酸果糖(FDP)和巯甲丙脯酸(CAP)增强心脏停搏液对缺血心肌保护的临床效果。方法 将60例患者随机分成三组。Ⅰ组:作为对照组,应用我院体外循环下心肌保护方法,即首剂应用冷钾晶体心脏停搏液,从第二剂量开始改用15℃稀释氧合血灌注;Ⅱ组:在冷钾晶体心脏停搏液中加入FDP(5mmol/L);Ⅲ组:在冷钾晶体心脏停搏液中加FDP(5mmol/L)和CAP(12.5mg/L)。观察血浆丙二醛(MDA)、肌酸磷酸激酶同工酶(CPK-MB)、血栓素B2(TXB2)、6-酮-前列腺素F1α(6-酮-PGF1α)及电子显微镜检查结果。结果 与Ⅰ组比较,Ⅱ组和Ⅲ组MDA,CPK-MB明显降低,且Ⅲ组较好地维持了TXB2和6-酮-PGF1α二者的比例平衡。Ⅱ组和Ⅲ组对线粒体也有较好地保护及提高毛细血管通畅率的作用。结论 FDP和CAP能明显增强心脏停搏液对缺血心肌保护的效果。

    Release date:2016-08-30 06:33 Export PDF Favorites Scan
  • Clinical Outcomes of Cold Blood Cardioplegia and Intermittent Cross Clamping as Myocardial Preservation in Coronary Artery Bypass Grafting

    Objective\ In order to assess and evaluate the clinical results of cold blood cardioplegia and intermittent cross clamping as myocardial preservation in coronary artery bypass grafting(CABG).\ Methods\ According to the management methods, 2 013 cases for elective, isolated CABG were divided into two groups at St.George’s Hospital, London.Cold blood cardioplegia group: 596 patients treated with cold blood cardioplegia, and hypothermic ventricular fibrillation group: 1 417 patients treated with intermitt...

    Release date:2016-08-30 06:35 Export PDF Favorites Scan
  • 含氧血心脏停搏液持续灌注后血清钾的变化

    目的 观察含氧血心脏停搏液持续灌注后血清钾的变化,对造成高钾血症的原因及预防措施进行了探讨.方法 根据灌注不同的心脏停搏液,将68例心瓣膜直视术患者随机分为两组,组Ⅰ:用冷晶体心脏停搏液(St.Thomas液)灌注;组Ⅱ:用含氧血心脏停搏液灌注.对两组血清钾水平进行了连续监测及比较分析. 结果 含氧血心脏停搏液持续灌注者术后能使血清钾维持在较好的水平,但有可能造成术中高钾血症. 结论 使用含氧血心脏停搏液持续灌注技术适当,可避免高钾血症.

    Release date:2016-08-30 06:35 Export PDF Favorites Scan
2 pages Previous 1 2 Next

Format

Content