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find Keyword "晶体" 31 results
  • 氧合冷血与St.Thomas心脏停搏液逆行灌注对心肌保护的比较

    目的对比氧合冷血与St.Thomas心脏停搏液持续逆行灌注对心肌保护的影响。方法选择2004年9月至2005年3月在我院行心瓣膜置换术患者74例,其中双瓣膜置换术11例,二尖瓣置换术(MVR)43例,主动脉瓣置换术(AVR)20例。74例被随机分为两组,A组:34例,St.Thomas停搏液持续逆行灌注;B组:40例,氧合冷血停搏液持续逆行灌注。于手术前、主动脉阻断即刻、主动脉开放即刻、术后2、6、12、24、48和72h测中心静脉血心肌肌钙蛋白I(cTnI)、肌酸激酶(CK)、肌酸激酶同工酶(CK—MB)、乳酸脱氢酶(LDH)。观察心脏复跳情况,并将两组间测量结果进行比较。结果术前两组cTnI、CK、CK—MB、LDH水平均在正常范围,开放主动脉后2h开始升高,48h后缓慢下降。两组间开放主动脉后2、6、12、48和72h,cTnI、CK、CK—MB、LDH测定结果比较差别有统计学意义(P〈0.05)。A组心脏自动复跳20例(58.82%),B组40例(100%),两组比较差别有统计学意义(P〈0.05)。结论与St.Thomas冷晶体心脏停搏液相比,氧合冷血心脏停搏液持续灌注具有更好的心肌保护作用。

    Release date:2016-08-30 06:22 Export PDF Favorites Scan
  • 冷晶体间断灌注与联合温血诱导停搏及终末再灌注的对比研究

    目的 对比温氧合血诱导停搏及终末再灌注和4℃冷晶体心脏停搏液间断灌注的心肌保护作用. 方法 将40例风湿性心脏病患者随机等分成温血组和冷晶体组,观察心脏自动复跳率,测定停搏前、复跳后0小时、6小时、12小时、18小时、24小时和48小时的血浆心肌肌钙蛋白I(cTnI),对心肌线粒体作量化计分. 结果 温血组心脏自动复跳率明显高于冷晶体组;体外循环后温血组cTnI明显低于冷晶体组,温血组 cTnI高峰出现时间较早;线粒体量化计分分数反映复跳后温血组心肌损伤较轻. 结论 温氧合血诱导停搏及终末再灌注的心肌保护效果较好.

    Release date:2016-08-30 06:32 Export PDF Favorites Scan
  • Abnormal expression of αA-crystallin in neural retina in type 2 diabetic rats

    Objective To observe the abnormal expression of alpha;A-crystallin protein in neural retina in type 2 diabetic rats via proteomic technique.Methods Twenty-eight male Sprague-Dawley (SD) rats were randomly divided into the normal control and the diabetic experimental groups with 14 rats in each group.A type 2 diabetes rat model (T2DM) was set up in the diabetic experimental group by feeding high fat diet combined with peritoneal injection of low dose streptozotocin (STZ);the successful diabetes model is with the randomlydetected blood glucose of >16.7 mmol/L.The rats in the control group underwent peritoneal injection of equivalent sodium citrate solution and were fed with normal diet.All of the animals were sacrificed by decapitation 56 days after the induction of diabetes.The eyes were enucleated and the neural retina layers were carefully peeled off and preserved.The total neural retinal proteins were extracted from the control and diabetic groups, respectively,and then subjected to two dimensional gel electrophoresis (2-DE).Some different proteins spots were identified by peptide mass fingerprinting (PMF) as well as by tandem mass spectrometric (MS/MS) measurements.Western blot and indirect immunofluorescence (IMF) were used to confirmed that alpha;A-crystallin protein expression was upregulated in diabetic retina.Results An average of (3122plusmn;37) spots in normal retinas and(2702plusmn;21)spots in diabetic group were found by 2-DE image analysis software; about 150 spots in 2-DE gel of diabetic retinae exhibited statistically significant variations (t>2.77,P<0.05).Compared with normal rats' retinae, diabetic ones presented 68 protein spots of up regulation expression and 82 of downregulation expression in 2DE gel.Furthermore,20 of the 150 protein spots were identified by mass spectrometry.The points of 2369 and 1048 in 2-DE gel, showing high expression in diabetic retinal tissues, were identified as alpha;A-crystallin via PMF.Western blot validated that the expression level of alpha;A-crystallin in diabetic neural retina was much higher than that in the control group. Significantly increased expression of alpha;A-crystallin in nuclear retina in diabetic group was also observed by IMF. Fluorescence was mainly seen in the retinal nuclear layer;alpha;A-crystallin aggregation was detected in the perinuclear region of neurons.Conclusion The expression of alpha;A-crystallin increases in neural retina of early T2DM rats.

    Release date:2016-09-02 05:40 Export PDF Favorites Scan
  • Expression of αA and αB crystallin protein in retina after blue light exposure

    Objective To observe the expression of alpha;A-and alpha;B-in retina after blue-light exposure.Methods  Forty female Wistar rats were divided randomly into 4 groups:control group,and blue-light exposure for 6,12,and 24 hours groups, with 10 rats in each group. The rats in the control group were not intervened.The other three groups of rats were exposed to blue fluorescent lights for 6,12,and 24 hours respcetively. Then the rats were kept in darkness for 12 hours. The globes were enucleated after anaesthesia.The immunohistochemistry and Western blot were performed to detect the expression of alpha;A and alpha;B-crystallin in retina.Results The absorbance value (A value) of retina alpha;A-crystallin was 1.40573plusmn;0.70748 in the control group, and were 4.317 51plusmn;0.412 97, 7.397 08plusmn;1.947 90, 9.634 32plusmn;2.377 61, respectively in the other 3 groups; the difference among the groups was significant (F=24.569,P<0.001). The A value of retina alpha;B-crystallin is 0.129 36plusmn;0.033 93 in the control group, and were 0.507 17plusmn;0.117 55, 7.345 43plusmn;2.292 97, 4.042 26plusmn;3.890 23, respectively in the other 3 groups; the difference among the groups was significant(F=40.102,P<0.001). The results of Western blot showed that the expression of alpha;A and alpha;B crystallin in groups with bluelight exposure was obviously higher than that in the control group.Conclusions Blue light may up-regulate the expression of alpha;A-and alpha;B-crystallin in ratsprime; retina.

    Release date:2016-09-02 05:40 Export PDF Favorites Scan
  • Auto-cortex of crystalline lens-induced neovascular epiretinal memb rane national

    Objective To investigate auto-cortex of crystalline lens-induced neovascular epiretinal membrane(NVERM)by micro-injuring posterior c apsule of crystalline lens. Methods twenty four C57BL/6 mouse between 4-6 weeks were selected, and divided into two groups randomly: auto-cortex of crystalline group and the control group. The auto-cortex of crystalline group was treated by penetrating the posterior capsule of lens and washing out the lens cortex into the mouse vitreous using PBS (phosphate buffered solution), while the control group were injected PBS into vitreous merely. Clinical change s were followed by slit-lamp examination and photograph. The eye balls were enu cleated at the day of 3, 7, 14 and 28 after operation. Both HE and immunohistoch emistry were used to detect the pathological changes. Results postoperative one to three days, 11 of 12 mouse in autocortex of crystalline g roup, lens appear to alba turbid at different levels one after another, and then develop into highdensity chinaware white. Postoperative (po) three days, HE s taining shows cortex of lens debris transmigrated in vitreous cavity, and some o f which approached to internal limiting membrane and lead it to rough and discon tinue; Po7-14 days, the capillary in retina expanded, migrated and broke though t internal limiting membrane which got to the pro retina and became the new ves sels. And typical NVERM were observed. Po28 days, some vascularslike structure formed in vitreous cavity. None of mouse in control group developed NVERM. Conclusion Auto-cortex of crystalline lens can induced neovascular epiretinal membrane in C57BL/6 mouse.  (Chin J Ocul Fundus Dis,2008,24:118-121)

    Release date:2016-09-02 05:46 Export PDF Favorites Scan
  • 玻璃体切割术治疗人工晶状体眼的视网膜脱离

    Release date:2016-09-02 05:58 Export PDF Favorites Scan
  • Clinical observation on preservation of anterior lens capsule in the reduction of complications associated with silicone oil

    Objective To observe the effect of preservation of an terior lens capsule on the incidence of complications associated with silicone oil. Methods Eighty-two patients(82 eyes)accepted trans pars plana vitrectomy combined with lensectomy,30 eyes with preservation of an terior lens capsule (PAC) and 52 eyes with no preservation of anterior capsule(N PAC)were observed.The incidence of complications was analysed to investigate whe ther PAC could reduce the complications associated with the usage of tamponade of silicone oil. Results The incidence was 50.0% in NP AC group,and 23.3% in PAC group(0.010lt; Plt; 0.025).There were secondary glaucoma(21.1%),band keratopathy(13.5%)and corneal decompensation(9.6%)in NPAC group,while there was none of them in PAC group. Conclusion Preservation of anterior lens capsule is an effective measure to reduce the complicaltons associated with the tamponade of silicone oil. (Chin J Ocul Fundus Dis, 2001,17:41-43)

    Release date:2016-09-02 06:03 Export PDF Favorites Scan
  • 眼球穿孔伤人工晶状体手术后视网膜脱离

    Release date:2016-09-02 06:05 Export PDF Favorites Scan
  • 雷公藤多甙对兔眼人工晶体植入后虹膜睫状体超微结构变化的影响

    Release date:2016-09-02 06:11 Export PDF Favorites Scan
  • Retinal detachment after extracapsular cataract extraction with posterior chamber intraocular lens

    Objective To approach the clinical characters and therapeutic methods of retinal detachment(RD) after extracapsular catarat extraction(ECCE)with posterior chamber intraocular lens(PCIOL). Methods Sixty eight cases(68 eyes) of RD after ECCE with PCIOL were treated with sclerel buckling,microvitreo retinal surgery and intraocular gas,silicone oil injection and were reviewed. Results The retinas were totally reattached in 65 eyes(95.59%) which dropped to 94.12% in 6-60 months postoperatively.The resultant rate of visual acuity of the eyes with 0.1 or better was 79.41%,with 0.3 or better was 26.47%. Conclusion The main causes of RD after ECCE with PCIOL are similar to those of general RD,and most cases of RD after ECCE with PCIOL can be cured by surgical treatment. (Chin J Ocul Fundus Dis,1998,14:167-169)

    Release date:2016-09-02 06:11 Export PDF Favorites Scan
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