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find Author "曹阳" 12 results
  • STUDY ON THE EFFECT OF SPINAL NEURAL PROGENITOR TRANSPLANTATION ON TREATING BRACHIAL PLEXUS AVULSED INJURY

    Objective To explore the effect of spinal neural progenitor transplantation to the cervical spinal on treating brachial plexus injury with the reimplantation of the avulsed spinal roots. Methods Thebrachial plexusavulsed injury model was made on 54 rats and they were evenly divided into 3 groups: fresh group, chronic group, control group. The spinal neural progenitor was cultured and identified. Then 10 μl(1×105/μl)cells were labelled with BrdUand transplanted into the fresh group (15 rats survived, being model for 1 week) and the chronic group (14 rats survived, being model for 2 months). No cell was transplanted into the control group. Two months after the transplantation, therecovery of function of the injured limb was evaluated. Electrophysiologic study and immunohistochemical study of the injured limb were made. Results Spinal neural progenitors were isolated from the spine and became neural sphere. The neural spheres were differentiated into neurons and astrocytes. Fourteen rats out of 15 in the fresh group were recovered, 7 rats out of 14 in the chronic groupwere recovered, and 5 rats out of 12 in the control group were recovered. Immunohistochemical study indicated that the transplanted progenitors in fresh group survived and differentiated into the neural cells, and the transplanted progenitors in chronic group existed and did not differentiate well. Conclusion Transplanted spinal neural progenitors can promote the recovery of the brachial plexus injury with the reimplantation of the avulsed spinal root.

    Release date:2016-09-01 09:28 Export PDF Favorites Scan
  • 前臂桡动脉逆行岛状皮瓣加游离植皮修复手部脱套伤

    Release date:2016-09-01 09:35 Export PDF Favorites Scan
  • THE CHARACTERISITICS OF PLASMA-SPRAYED COATINGS OF HYDROXY APATITE IN VITRO

    In order to investigate the stability of Hydroxyapatite (HA) coated material, the plasma-sprayed coatings of HA were divided into four groups: 1. Keeping in water vapour at 125 degrees C, with a pressure of 0.15 MPa for 6 hr; 2. Heating at 650 degrees C in air for half an hr; 3. Keeping in water vapour at 490 degrees C, with a pressure of 0.01 MPa for 2 hr; and 4. The control. The XRD, FTIR analysis and the dissolution test were carried out. The results showed: 1. The degree of crystalization in XRD analysis was 3. gt; 1. gt; 2. gt; 4.; 2. The ampitude of OH- peak in FTIR analysis was 3. gt; 1. gt; 2. gt; 4. and 3. The dissolution rate in tris-HCl buffer was 3. lt; 1. lt; 2. lt; 4. The conclusions were 1. The treating with water vapour could decrease the transformation temperature which was needed to convert the amorphous phase into cystalline phase; 2. Water vapour treatment could accelerate the transformation of Z-TCP, TCPM into crystalline HA; 3. Water vapour treatment could promote the structural integrity of plasma--sprayed coated of and HA and 4. Water vapour treatment could lower the dissolution rate of HA coated in Tris-HCl buffer.

    Release date:2016-09-01 11:07 Export PDF Favorites Scan
  • Filtering Method of Power Frequency Interference in ECG Signals

    Electrocardiogram (ECG) signals are susceptible to be disturbed by 50 Hz power frequency in the process of acquisition and conversion. This paper puts forward the improved Levkov algorithm combined with the antisymmetry of sine wave and the characteristic of not-easily mutation of the sine wave amplitude. The improved and the original algorithms were compared with signal to noise ratio (SNR), root mean square error (RMSE) and coincidence of the signals. Based on the experimental comparison, it was concluded that the improved algorithm had better filtering functions, because it not only highly coincided with ECG, but also retained certain details of ECG.

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  • A new method for calculating pulse oxygen saturation

    Real-time updates of metrical data can not generally be realized in the commonly used methods for calculating the pulse wave of blood oxygen saturation. Based on the hardware platform of pulse wave signal from NJL5501R, and high linear correlation of the red laser and infrared light collected in pulse wave signal measurement, an approach to determine the value of the blood oxygen saturation is proposed in the present paper by establishing the linear regression model of the red laser and infrared light. The effect of the sampling number of pulse wave signal in calculation on the characteristic parameters of pulse wave is also analyzed. The experimental results showed that the approach could guarantee the measuring accuracy and realize the fast updates of blood oxygen saturation data. This paper provides an effective method for real-time and accurate monitoring of pulse blood oxygen saturation in human body.

    Release date:2017-04-01 08:56 Export PDF Favorites Scan
  • Effects of hypoxia-inducible factor 1α on hypoxic tolerance of human amniotic mesenchymal stem cells

    ObjectiveUnder hypoxic conditions, the survival and apoptosis of human amniotic mesenchymal stem cells (hAMSCs) were observed by transient transfection of hypoxia-inducible factor 1α (HIF-1α) gene, to investigate the effect of HIF-1α on hypoxic tolerance of hAMSCs.MethodsThe hAMSCs were isolated and cultured from amniotic membrane tissue from voluntary donors who were treated with cesarean section. And the morphological observation by inverted phase contrast microscope and immunofluorescence detection of the expressions of stem cell markers OCT-4 and NANOG were performed to identify the cultured cells. The third generation hAMSCs were treated with 200 μmol/L CoCl2, and transient transfection of plasmids were added according to the following grouping: group A was hAMSCs blank group; group B was pcDNA3.1 negative control group; group C was short hairpin RNA (shRNA) negative control group; group D was shRNA-HIF-1α interference group; group E was pcDNA3.1-HIF-1α over expression group. Cell survival rate of each group was measured by cell counting kit 8 (CCK-8) at 12, 24, 48 hours after hypoxia treatment. Flow cytometry was used to detect apoptosis rate of each group at 24 hours after hypoxia treatment. The expression levels of HIF-1α, vascular endothelial growth factor (VEGF), B-cell lymphoma 2 (Bcl-2), Bax, and cleaved Caspase-3 (C-Caspase-3) proteins were detected by Western blot at 24 hours after hypoxia treatment.ResultsCCK-8 assay showed that the cell survival rate of group D was significantly lower than those of groups A and C at all time points after hypoxia treatment; while the cell survival rate in group E was significantly increased than those in groups A and B, and the diffrences at 24 hours were significant (P<0.05). In group E, the cell survival rate at 24 hours was significantly higher than those at 12 and 48 hours (P<0.05). The results of flow cytometry showed that the apoptosis rate in group D was significantly higher than those in groups A and C (P<0.05), and the apoptosis rate in group E was significantly lower than those in groups A and B (P<0.05). Western blot showed that the expressions of HIF-1α, VEGF, and Bcl-2 proteins in group D were significantly decreased when compared with those in groups A and C, and the expressions of Bax and C-Caspase-3 proteins were significantly increased (P<0.05). On the contrary, the expressions of HIF-1α, VEGF, and Bcl-2 proteins in group E were significantly higher than those in groups A and B, and the expressions of Bax and C-Caspase-3 proteins were significantly decreased (P<0.05).ConclusionOverexpression of HIF-1α gene can significantly improve hAMSCs tolerance to hypoxia, the mechanism may be related to up-regulation of VEGF and Bcl-2 expressions, and down-regulation of Bax and C-Caspase-3 expressions.

    Release date:2018-03-07 04:35 Export PDF Favorites Scan
  • Irbesartan combined with metoprolol compared with single-drug in the treatment of elderly patients with severe heart failure: a network meta-analysis

    ObjectiveTo systematically review the efficacy of irbesartan combined with metoprolol plus conventional treatment compared with single drug (irbesartan, metoprolol) plus conventional treatment or conventional treatment for elderly patients with severe heart failure.MethodsPubMed, The Cochrane Library, Web of Science, CNKI, WanFang Data and VIP databases were electronically searched to collect randomized controlled trials (RCTs) on irbesartan combined with metoprolol in the treatment of elderly patients with severe heart failure from January 2009 to September 2019. Two reviewers independently screened literature, extracted data and assessed the risk of bias of included studies. Network meta-analysis was then performed using gemtc package of R software.ResultsA total of 58 RCTs involving 5 064 patients were included. The results of meta-analysis showed that the efficiency, left ventricular ejection fraction (LVEF) and brain natriuretic peptide (BNP) of combination group were significantly superior to single drug group.ConclusionsThe current evidence shows that under the premise of conventional treatment, the combination of irbesartan and metoprolol is more effective than irbesartan or metoprolol alone in the treatment of elderly patients with severe heart failure. Due to limited quality and quantity of the included studies, more high quality studies are required to verify above conclusions.

    Release date:2020-07-02 09:18 Export PDF Favorites Scan
  • 诊断主动脉夹层动脉瘤的影像学比较

    【摘要】 目的 讨论彩色多普勒超声、多层螺旋CT(multislice spiral CT,MSCT)及MRI对主动脉夹层动脉瘤(aortic disection,AD)的诊断价值,评价3种检查方式的优势与不足,为临床选择检查提供依据。 方法 回顾性分析2008年1月—2010年6月期间,32例经手术证实为AD患者的彩色多普勒超声、MSCT及MRI检查资料,并与术中所见及病理分型进行对比分析。 结果 术前彩色多普勒超声检出率为78.1%(25/32),诊断准确率为84%(21/25),MSCT及MRI检出率及诊断准确率均为100%。 结论 3种检查方法对AD术前均有较高的诊断价值,彩色多普勒超声术前诊断准确率及检出率低于MSCT及MRI,应存患者病情允许情况下,MSCT及MRI为首选检查方法,若病情危重则以床旁彩色多普勒超声为最佳检查方法,3种检查在临床应用上各有优势与不足,具体选择应视患者病情而定。

    Release date:2016-09-08 09:26 Export PDF Favorites Scan
  • A Heart Rate Variability Analysis System for Short-term Applications

    In this paper, a heart rate variability analysis system is presented for short-term (5 min) applications, which is composed of an electrocardiogram signal acquisition unit and a heart rate variability analysis unit. The electrocardiogram signal acquisition unit adopts various digital technologies, including the low-gain amplifier, the high-resolution analog-digital converter, the real-time digital filter and wireless transmission etc. Meanwhile, it has the advantages of strong anti-interference capacity, small size, light weight, and good portability. The heart rate variability analysis unit is used to complete the R-wave detection and the analyses of time domain, frequency domain and non-linear indexes, based on the Matlab Toolbox. The preliminary experiments demonstrated that the system was reliable, and could be applied to the heart rate variability analysis at resting, motion states. etc.

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  • EFFECTS OF BONE MARROW MESENCHYMAL STEM CELLS TRANSPLANTATION ON EXPRESSION OF VASCULAR ENDOTHELIAL GROWTH FACTOR GENE AND ANGIOGENESIS AFTER SPINAL CORD INJURY IN RATS

    Objective To investigate the effect of bone marrow mesenchymal stem cells (BMSCs) transplantation on the motor function recovery, the expression of vascular endothel ial growth factor (VEGF) gene, and angiogenesis after spinal cord injury (SCI) in rats, and to explore the treatment mechanism of BMSCs in SCI. Methods BMSCs were isolated and cultured from the marrow of 5 Wistar rats (4 weeks old) and the 3rd-4th passage cells were prepared for the experiment. Atotal of 87 adult female Wistar rats (weighing 220-250 g) were randomly divided into 3 groups: sham-operated group (group A, n=21), DMEM group (group B, n=33), BMSCs group (group C, n=33). A laminectomy was only performed at T8-10 levels in group A. The SCI models were establ ished by modified Nystrom’s compression method in groups B and C, and BMSCs and DMEM were injected in groups B and C respectively at 30 minutes after SCI. Basso-Beattie-Bresnahan (BBB) score was used for the motor function recovery at 3, 7, 14, and 28 days, RT-PCR for the VEGF mRNA at 1, 3, and 5 days, and immunohistochemical staining for angiogenesis at 3, 7, 14, and 28 days. Results In groups B and C, the hindl imb locomotor function was improved at different degrees with time, showing significant difference in BBB score between groups B, C and group A (P lt; 0.05). At 28 days, the BBB score in group C was significantly higher than that in group B (P lt; 0.05) and there was no significant difference between groups B and C (P gt; 0.05) at 3, 7, and 14 days after transplantation. The numbers of microvessels in the ventral horns of gray matter around SCI in groups B and C were significantly lower than that in group C (P lt; 0.05) at 3 days, but there was no significant difference at 7, 14, and 28 days after transplantation (P gt; 0.05). There was no significant difference in the number of microvessels between group C and group B (P gt; 0.05) at 3 and 7 days, but the number of microvessels in group C was significantly higher than that in group B (P lt; 0.05) at 14 and 28 days after transplantation. However, there was no significant difference in the number of microvessels in the white matter around SCI in 3 groups at different time points after transplantation (P gt; 0.05). The RT-PCR results showed that VEGF mRNA expressed at a low level in group A. Compared with group A, the expression level of VEGF mRNA in groups B and C increased at 1 day and reached the peak at 3 days, then decreased at 5 days after transplantation; and the expression of VEGF mRNA was significantly higher in groups B and C than in group A (P lt; 0.05),and in group C than in group B (P lt; 0.05) at 1, 3, and 5 days. Conclusion BMSCs may promote the motor function recoveryby up-regulating VEGF mRNA expression and increasing angiogenesis in the spinal cord after SCI in rats.

    Release date:2016-08-31 05:44 Export PDF Favorites Scan
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