Objective To determine the concentration of hepatocyte growth factor (HGF) in vitreous of diabetic retinopathy (DR) and investigate the result and influence of HGF in neovascularization in proliferative diabetic retinopathy (PDR). Methods The high sensitive sandwich enzyme-linked immunosorbent assay (ELISA) was used to measure the level of HGF in vitreous of normal group for 10 eyes, simple DR group for 7 eyes, PDR without iridal neovascularization group for 24 eyes, PDR with neovascularization of iris group in 9 eyes, other retinopathy group associated with neovascularization for 8 eyes. Results The mean value of HGF level in vitreous of the former groups was: (3.3 ±1.9) μg／L in normal group; (4.8±2.5) μg／L in simple DR group; (13.0 ±5.2) μg／L in PDR without iridal neovascularization group; (18.6±7.2) μg／L in PDR with neovascularization of iris group；(12.1±8.9) μg／L in other retinopathy associated with neovascularization group. Stastistics showed that HGF level in PDR group and other retinal diseases associated with neovascularization were significantly higher than that in normal group (P＜0.01), PDR with neovascularization of iris group showed a higher mean vitreous HGF concentration than those in PDR without iridal neovascularization and simple DR group (P＜0.05 or P＜0.01). Conclusion Our results indicate that vitreous HGF may play an important role in retinal neovascularization in PDR and other retinal diseases. (Chin J Ocul Fundus Dis, 2002, 18: 131-133)
Objective To investigate the characteristics of fundus angiography with fluorescein and indocyanine green in reattachment of rhegmatogenous retinal detachment． Methods Fundus fluorescein angiography(FFA)and indocyanine green angiography(ICGA)were performed on 21 cases with simple rhegmatogenous retinal detachment postoperatively． Results (1) On the artificial ridge of 21 cases,window defect hyperfluorescence was found in FFA,choroidal vessel dilatation and choroidal filling defect of 14 cases were showed in ICGA;(2) In 6 cases cryocoagulation,fluorescein leakage was showed in FFA and ICGA,leakage of choroid was observed in late stage of ICGA;(3) In 6 cases photocoagulation,a few fluorescein leakage was demonstrated in FFA and none of this feature was found in ICGA;(4) ICGA showed slow filling of choroid of perioptic disc in 2 case,and temporo-inferiorly in 2 case. Conclusion Window defect hyperfluorescence of FFA and choroidal vessel dilatation of ICGA on the artificial ridge were the main characteristics in reattachment of rhegmatogenous retinal detachment. (Chin J Ocul Fundus Dis,20000,16:17-19)