【摘要】 目的 了解在围手术期术前30 min应用抗生素的情况。 方法 根据2004年卫生部、国家中医药管理局、总后勤部发布的《抗菌药物临床应用指导原则》中围手术期抗生素的使用原则，对四川大学华西医院2010年4-6月500台手术围手术期抗生素的使用情况进行分析。 结果 抗生素在麻醉前输：0台；抗生素末在术前30 min输2台，占0.4%；抗生素末即用即配：0台；抗生素与麻药及其他禁忌药混合输:0台；手术3 h后末及时追加抗生素：0台；特殊患者使用抗生素的注意事项不清楚2台，占0.4%。 结论 该院99.6%的手术实行在手术室术前30 min输入抗生素，确保抗生素达到有效浓度，有效控制感染，保证手术的成功，保障患者安全。【Abstract】 Objective To investigate the application of perioperative antibiotics half an hour before operation in West China Hospital of Sichuan University. Methods According to Clinical Guidance of Antibiotics published by Ministry of Health, State Administration of Traditional Chinese Medicine and General Logistics Department in 2004, we investigated the application of perioperative antibiotics in 500 operations between April to June 2010 in our hospital. Results There was no operation with infusion of antibiotics before anesthesia, 2 operations without infusion of antibiotics half an hour before operation (0.4%), no operation without immediate infusion after preparation, no operation with mixed infusion of antibiotics and anesthesia and other contraindicated drugs, no operation without infusion of antibiotics 3 hours after operation, and 2 operations in which cautious items about the children, pregnancy and old patients were unclear (0.4%). Conclusion About 99.6% operations in our hospital have the infusion of antibiotics 30 minutes before the operation, which is the guarantee of antibiotics with effective concentration, inhibition of infection, success of the operation and safety of the patients.