ObjectiveTo establish multidrugresistance cell substrain of human hepatocellular carcinoma and to investigate its characteristics.MethodsSMMC7721 cell strain was cultured in Adriamycin(ADM). The multidrugresistance cell substrain SMMC7721/ADM was harvested after a long period of culture by gradually increasing the concentration of ADM and its characteristics were investigated. Results①The drug resistance of SMMC7721/ADM to ADM increased by 33.3 times, to Vincristine 16.8 times, to Diamminedichloroplatinum 2.8 times. ②The drug resistance cell substrain had almost the same growth velocity as its parental generation. The doubling time was 32.0 hours and 30.5 hours respectively. They had the analogous growth curves. ③The obvious difference between the drug resistance cell substrain and its parental generation was that the former’s microvilli became thick, short and scattered by scanning and transmitting electron microscopy. ④The multidrug resistance cell substrain kept the characteristics of hepatocellular carcinoma, it could be transplanted into the subcutaneous tissue of nude mice. ⑤The drug resistance of the cell substrain reduced to 28.0% and 9.2%after removal of the drug for 1 month and 2 months respectively, its drug resistance could remain stable (35.4 times) after 2 months of culture in ADM (0.04 μg/ml).ConclusionThe SMMC7721/ADM cell substrain has the stable fundamental characteristics of a drug resistance cell strain.
ObjectiveTo analyze the health examination results of hospital retirees, understand their health status and provide the evidence for health management. MethodsThe data were collected from our 1 089 hospital retirees (51-96 years old) who received health examination in West China Hospital of Sichuan University from January to December 2013, including 345 males and 744 females, with a mean age of 70 years. The data were analyzed by SPSS 16.0 software. ResultsHypertension, dyslipidemia and diabetes were the three chronic diseases with the highest detectable rate, and the rate was respectively 49.49%, 44.90% and 31.04%. The detectable rate of hypertension was not significantly different between male and female. The rate of dyslipidemia in females was higher than that in males. The rate of diabetes in males was higher than that in females. The detectable rate of hypertension and diabetes increased with the increase of age. The detectable rate of dyslipidemia had no significant relationship with age. ConclusionThe health condition of retirees cannot be neglected. We need to strengthen the health management for the retirees.