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find Keyword "模型, 动物" 8 results
  • 关于本刊稿件录用和编辑加工问题的几点说明

    本刊以推动眼底病学术发展为已任,整个编辑出版活动均是以此出发点为基础,自然也有一些体现自身特色的稿件录用原则及编辑加工送修要求。为了方便作者投稿,现将其中的一些具体问题说明如下: 1 稿件评审和录用 本刊稿件实行严格的双盲同行评审,同行评审结果是确定稿件录用与否的基本准则。在多位同行专家评审的情况下,如果其评审结果有分歧,会以多数专家的评审意见作为主导。尽管这种评审方式也可能会因各种原因出现一些评审结果的偏差,但在科学技术评价这一复杂的系统工程还没有尽善尽美之前,发现和克服上述可能带来偏差的消极因素是我们不断努力的方向。也请作者正确理解和妥善处理稿件评审结果以及由此形成的退稿和稿件送修意见。具体工作中,存在一些影响稿件录用的参考因素。(1)体现办刊特色的因素。在上述同行评审基础上,符合本刊报道计划的专题号文稿会优先录用;而非专题号文稿中,临床研究文稿较实验研究的文稿更受欢迎;各种基金资助的文稿同等条件下更容易被接纳。(2)区域差异和普及与提高的因素。在质量基本保证的情况下,来自相对发展滞后的地区以及基层医院的同样性质文稿可能会比来自中心城市大医院的文稿相对更容易发表。(3)满足作者发表功利的因素。我们充分理解作者发表文章的各种愿望,也乐意协助其实现文章发表的各种目的。但在做好为读者服务和为作者服务的具体工作中,我们会首先考虑为读者服务、为推动学术发展服务。在杂志版面和出版周期等资源有限的情况下,对于少数作者出于毕业、晋升、成果鉴定等社会功利需要,对文章刊出时间和刊出形式提出的一些不符合本刊办刊宗旨的要求可能不会全部满足。提请有这些特殊需求的作者在投稿前充分注意到这一点。 2 稿件编辑加工 依照《中华人民共和国著作权法》以及新闻出版行业的有关规定,本刊将恪守杂志作为科学文化事业发展的守望者和把关人的基本准则,对所有通过同行评议的拟刊用文稿均要从学术质量和表达规范两个方面进行编辑加工。对于本刊编辑加工稿送修过程中给作者文章发表带来的时间以及精力上的压力或者是麻烦敬请理解。发表一篇真正有益于社会的好文章是作者和编辑共同努力的结果,也是我们大家的应尽之责。另外,由于编辑个人能力和编辑部整体管理水平有限,稿件编辑加工和送修流程上的不足之处还请作者包容理解,批评指正。做眼底病同仁的朋友,我们一直在努力;也希望大家与我们一道共同努力,一起推动眼底病事业的进步。

    Release date:2016-09-02 05:22 Export PDF Favorites Scan
  • 眼内光纤照明联合孟加拉玫红诱导兔视网膜分支静脉血栓

    Release date:2016-09-02 05:22 Export PDF Favorites Scan
  • Protective effects of recombinant erythropoietin on photoreceptor cells in rat with retinal detachment

      Objective To investigate the protective effect of recombinant erythropoietin (EPO) on the photoreceptor cells in rat with retinal detachment (RD).Methods One hundred and sixtytwo normal male rats were randomly divided into normal control (NC) group, RD model group, RD+phosphate buffer solution (RD+PBS) group, RD+EPO 100 ng group, RD+EPO 200 ng group and RD+EPO 400 ng group. Three days after RD, activated caspase3 and bclXL were detected by Western blot and/or immunofluorescence, and apoptosis were measured by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate digoxigenin nick-end labeling(TUNEL). Fourteen and 28 days and two months after RD, the outer nuclear layer (ONL)thickness was measured by histopathologic method.Results Western bolt indicated that the protein level of activated caspase-3 and bcl-XL between six groups were statistically significant(F=35.96, 30.75;P<0.01). The number of TUNEL positive cells and activated caspase-3 positive cells are consistent with each other in different groups. Fourteen days and two months after RD,the differences of ONL thickness between six groups were statistically significant(F=21.52,96.25;P<0.01).Conclusion Supplement of EPO after RD can alleviate apoptosis by inhibiting of the caspase-3 activity and increasing the expression of bcl-XL,thus exerts protective effect on photoreceptor cells.

    Release date:2016-09-02 05:41 Export PDF Favorites Scan
  • 视神经损伤激活视网膜干细胞的初步研究

    Release date:2016-09-02 05:41 Export PDF Favorites Scan
  • 尼莫地平对前部缺血性视神经病变大鼠眼内组织内皮素-1浓度的影响

    Release date:2016-09-02 05:41 Export PDF Favorites Scan
  • 应用液压冲击颅脑损伤仪建立大鼠外伤性视神经损伤动物模型

    Objective To observe whether the animal model of optic nerve injury in rats can be set up by fluid percussion brain injury device (FPI) or not.Methods Seventyone healthy female Wister rats were randomly divided into 2 groups, inlcuding model group with 66 rats and control group with 5 rats.The rats in model group were randomly divided into 3 groups. Eight rats in group 1 were examined by flashvisual evoked potential (F-VEP) and magnetic resonance imaging (MRI) examines before and 1, 3 days,1,2,4,6,and 8 weeks after injury; 56 rats in group 2 were randomly divided into 7 subgroups with 8 rats in each subgroup,and were detected by histopathological and terminal deoxynucleotidyl transferasemediated dUTP nick end labeling (TUNEL) apoptosis examines 1, 3 days, 1,2,4,6,8 weeks after injury;2 rats in group 3 were examined by electron microscopy 4 and 8 weeks after injury.According to the degree of injury, the injured eyes were divided into 2 groups including severe injury group with the beat pressure of (699.14plusmn;60.79) kPa and mild injury group with the beat pressure of (243.18plusmn;20.26) kPa.The right and left eyes in rats in each group were in severe and mild injury group, respectively.Results One day after injury, the latency duration of FVEP prolonged in severe injury group,wich differed much form which in the normal control group (P<0.05);the amplitude was gradually reduced during the first 2 weeks after injury and kept steady after that (P>0.05). The latency duration prolonged in mild injury group,and its difference with the normal control group was statistically significant (P<0.05);the amplitude was gradually reduced during the first 4 weeks after injury and kept steady after that (P>0.05). The abnormal high signal could be seen on optic nerve 1 day after injury, and was still obvious 8 weeks later. The results of histopathological examination showed ruptured capillary in ganglion cell layer 1 day after injury;retinal ganglion cells without nucleus could be seen 4 weeks after injury. The apoptosis of positive cells was found in each layer of the retina 3 days after injury.TUNEL results indicated that the number of apoptotic positive cells increased significantly 1-2 weeks after injury.Conclusion An animal model of optic nerve injury can be successfully set up using FPI in rats.

    Release date:2016-09-02 05:42 Export PDF Favorites Scan
  • The status and progress in gene therapy study of Stargardt disease

    Stargardt disease (STGD) is an inherited disorder of retinal pigment epithelium. Three genes have been found to be implicated in STGD including Abca4 (adenosine triphosphate-binding cassette, sub-family A, member 4), Elovl4 (elongation of very long chain fatty acids protein 4) and Prom1 (prominin-1). Target genes can be delivered to the retina by various methods such as lentivirus (LV) vectors, adeno-associated virus (AAV) vectors and non-viral nano-particles. The Abca4-/-, Elovl4-/- and Prom1-/- mice model are used to study the pathogenesis mechanism and treatment of STGD. Retinal function improved significantly upon gene therapy in these models. Based on these works using animal model, phase Ⅰ/Ⅱa clinical trial of Abca4-associated STGD gene therapy are underway. AsaLV vector, equine infectious anemia virus (EIAV) is used to carry the Abca4 gene. These studies will evaluate three dose levels of the EIAV vector for safety, tolerability and biological activity. Moreover, some preclinical attempts to deliver Abca4 via AAV have been made usingamodified AAV vectors because of the large size of the ABCA4 cDNA. The good responses in animal models render STGDavery attractive object for human gene therapy after the successful of the phase Ⅰ/Ⅱ clinical trials of Leber′s congenital amaurosis.

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  • Neuroprotective effects of benzatropine on rat model of nonarteritic anterior ischemic optic neuropathy

    ObjectiveTo investigate the neuroprotective effect of Benztropine on retinal ganglion cells (RGCs) death and optic nerve injury in rats model of non-arteritis anterior ischemic optic neuropathy (rNAION).MethodsA total of 25 Sprague-Dawley rats were randomly divided into Benztropine treatment group (n=13) and PBS control group (n=12). The right eye was set as the experimental eye. rNAION model was established by using rose Bengal combined with laser photodynamic method. The rats in the Benztropine treatment group were received intraperitoneal injection with Benztropine 10 mg/kg (0.2 ml) daily for 3 weeks, while the rats in the PBS control group were received intraperitoneal injection with an equal volume of PBS. At 1, 3 and 7 days after modeling, the retinal and optic disc conditions of the rats were observed by direct ophthalmoscopy. Retrograde labeling, fluorescence microscopy and transmission electron microscopy were used to observe the survival of RGCs and the damage of the optic nerve myelin and axon at 4 weeks after modeling. The RGCs density and survival rate of the two groups were compared by One-Way Anova.ResultsAt 1 and 3 days after modeling, the optic disc edema was observed in the rats of rNAION model group. At 7 days after modeling, the optic disc edema decreased and the boundary was blurred compared with 3 days after modeling.After 4 weeks, the RGCs density in the PBS group was 308±194/mm2 and the survival rate was 13.7%. The density of RGCs in the Benztropine group was 1173+868/mm2 and the survival rate was 47.6%. The differences of RGCs density and survival rate were significant between the two groups (F=7.552, 8.184; P=0.015, 0.012). Myelin disintegration, axon degeneration, onion-like body and gliosis were observed in the optic nerve sections of rNIAON in the PBS group, while the damage of axon and myelin structure in the Benztropine group was significantly less than that in the PBS group.ConclusionsBenztropine group showed higher RGC survival rate, less damage of axon and myelin structure on rNAION model. This study explored the potential neuroprotective effect of Benztropine.

    Release date:2019-05-17 04:15 Export PDF Favorites Scan
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