Abstract An animal experiment had been conducted for the purpose to find out the possibility of application of instantaneous sustained limited expansion (ISLE). A total of 54 skin defects in 9 pigs were obtained and were divided into two groups. One group of the pigs were subjected to instantaneous sustained limited expansion, the others, as the control group, just received the tension suture. At the same time the blood supply of the skin was measured by laser doppler flowmeter (LDF). It was showed that microcirculatory perfusion of the ISLE group was considerably better than that of the control. Inaddition, the correlation between the blood flow of the skin and the expanding pressure, as well as the pathologic changes of the skin were discussed.
Objective:To detect collagen I synthesis activity in the vitreous of PVR induced by macrophages in rabbits. Methods:PC Ⅲ (Procollagen Ⅲ ) concentrations were measured by radioim- munoassay in the vitreous samples of 14 rabbit eyes with experimental PVR and 14 control eyes. Results:The mean PC Ⅲ concentration on the 7th day after macrophage injection as 257.58mu;g/L(range,236.04~266.88mu;g/L,n= 4)and significantly increased on the 14th day later. On the 28th day the mean concentration of PC Ⅲ as 912.23mu;g/L (range, 881.36~943.10mu;g/L ;n= 2). There was a significant difference between the 7th and the 14th, 21st of 28th day statistically(P＜0.05). PC Ⅲ was not detected in control eyes. Conclusion:The PC Ⅲ level in the vitreous of rabbit eyes with experimental PVR increased significantly from the 7th to the 28th day after macrophages injection and is well consistent with the time course of scarring and the development of traction retinal detachment in the PVR model. （Chin J Ocul Fundus Dis,1996,12: 43-44）
目的:探讨简化生长激素药物激发实验的可行性。方法:对单独应用可乐定药物激发实验的结果和联合应用可乐定与精氨酸药物激发实验结果进行对照研究。GH峰值gt;10ng/mL,正常;GH峰值lt;5ng/ml,为GH完全缺乏;GH峰值在5 -10ng/ml之间,为GH部分缺乏。结果:简化药物激发实验与经典药物激发实验结果评估无显著性差异。结论:单独使用可乐定进行生长激素激发实验的结果与可乐定联合精氨酸进行生长激素激发实验结果评价无显著性差别。
【摘要】 目的 探讨在藏区白内障术前人工晶体度数的测定的必要性。方法 回顾性分析2009年8月—12月在拉萨光明眼科康复诊疗院进行白内障手术的170例患者,其中85例术前行人工晶体度数的测定,85例未行晶体度数的测定,比较两组术后3 d的视力。结果 测定组:术后视力≥0.3为51例,占60％；0.1lt;视力lt;0.3为24例,占28.24%;视力lt;0.1为10例,占11.76％。未测定组:术后视力≥0.3为48例,占56.47％；0.1lt;视力lt;0.3为17例,占20%;视力lt;0.1为20例,占23.53％。两组术后3 d最佳视力的比较有统计学意义(P＜0.05)。结论 术前测定人工晶体度数有助于改善白内障患者术后视力,在藏区有必要行白内障术前人工晶体度数的测定。
ObjectiveTo establish a method for content determination of ferulic acid in Piwang massage lotion. MethodsHigh performance liquid chromatography was used. The analysis was carried on Diamonsil C18 (150 mm×4.6 mm, 5 μm, Dimma Science and Technology Co. Ltd.) column with mobile phase consisted of methanol-2% acetic acid (32︰68). The column temperature was 30℃. The detection wavelength was 320 nm. ResultsThe responses of ferulic acid liner in range of 4.55-91.00 μg/mL (R2=0.999 9). The average recovery of ferulic acid was 98.78% (relative standard deviation is 2.668%). ConclusionThe method is simple, rapid and good repeatability. It can be used for quality control of Piwang massage lotion
Objective To evaluate the utility of collagen-gel droplet embedded-culture drug sensitivity test (CD-DST) in pancreatic carcinoma cell by compared with WST-8. Methods The chemosensitivity to 5-fluorouracil (5-FU), gemzar (GEM) and oxaliplatin (OXA) of pancreatic adenocarcinoma cells SW1990, PCT-3 and ASPC-1 were tested by WST-8 and CD-DST respectively. Results In a certain living cell number range (500-10 000), there was a linear correlation (r=0.991 1, P＜0.05) between the integral optical density in CD-DST and the cell number. The inhibition ratios of three kinds of cell growth tested by CD-DST were higher than those tested by WST-8 (P＜0.05). The results of drug chemosensitivity to 5-FU， GEM and OXA detected by two methods were uniform. Conclusion The CD-DST can be used to assay the drug chemosensitivity in vitro for pancreatic carcinoma.
目的 建立荣保灵芝1号子实体及其孢子粉中灵芝酸A的含量测定方法。 方法 采用高效液相色谱法，UltimateTM XB-C18色谱柱，柱温：25℃，流速：0.800 mL/min，检测波长：254 nm，流动相为乙腈(B)-0.1%冰乙酸水溶液(A)，梯度洗脱。 结果 灵芝酸A的线性范围是0.010～1.200 mg/mL（r=0.9998），平均回收率为100.55%，相对标准偏差为1.70%（n=6），子实体中灵芝酸A的含量范围在2.221 7～3.531 1 mg/g，孢子粉中灵芝酸A的含量范围在0.167 2～0.742 9 mg/g。 结论 该方法简便，专属性和重复性好，对连续3年采集的荣保灵芝1号子实体和孢子粉中的灵芝酸A进行测定，其含量都随年份的增加而增加。
Based on repeated experiments as well as continuous researching and improving, an efficient scheme to measure velocity and displacement of the coxa and knee movements based on video image processing technique is presented in this paper. The scheme performed precise and real-time quantitative measurements of 2D velocity or displacement of the coxa and knee using a video camera mounted on one side of the healing and training beds. The beds were based on simplified pinhole projection model. In addition, we used a special-designed auxiliary calibration target, composed by 24 circle points uniformly located on two concentric circles and two straight rods which can rotate freely along the concentric center within the vertical plane, to do the measurements. Experiments carried out in our laboratory showed that the proposed scheme could basically satisfy the requirements about precision and processing speed of such kind of system, and would be very suitable to be applied to smart evaluation/training and healing system for muscles/balance function disability as an advanced and intuitional helping method.