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find Keyword "浓缩" 4 results
  • 腹水回输加黄芪治疗肝硬化失代偿期患者肾功能改善效果

    目的 观察腹水回输腹腔加黄芪注射液治疗对肝硬化失代偿期伴腹水患者肾功能的改善情况。 方法 2006年3月-2008年5月住院的肝硬化失代偿期伴腹水、并有肾功能损害患者89例。随机分为两组,治疗组45例采取腹水超滤浓缩腹腔回输,同时给予黄芪注射液静脉滴注20 mL/d,常规保肝、利尿、支持治疗;对照组44例在常规保肝、利尿、支持治疗的同时间断放腹水。观察两组患者尿量、尿素氮和肌酐的情况。 结果 治疗前两组患者尿量、尿素氮和肌酐无明显差异,治疗后,治疗组患者尿量增加,血清尿素氮,肌酐下降,与对照组比较有差异。 结论 腹水超滤浓缩腹腔回输加静脉滴注黄芪注射液,能够明显改善肝硬化伴肾功能损害患者的肾功能。

    Release date:2016-09-08 09:47 Export PDF Favorites Scan
  • NHE3在胆固醇结石胆囊组织中的表达

    目的研究NHE3在正常和胆固醇结石患者胆囊黏膜上皮细胞中的表达情况,并探讨NHE3表达异常与结石形成的关系 方法利用Western blot以及免疫组织化学方法检测10例正常与16例胆固醇结石患者胆囊黏膜上皮细胞中NHE3蛋白和亚细胞定位的表达情况。 结果Western blot和免疫组织化学染色方法均显示,与正常胆囊组相比,结石组胆囊黏膜上皮细胞中NHE3表达增高(P<0.05),NHE3同时表达于胆囊黏膜上皮细胞的顶质膜和胞浆内。 结论NHE3表达异常可能与胆囊胆固醇结石发病相关,其发生机理有待进一步研究证实。

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  • Effect of concentrated growth factor combined with mineralized collagen material on the adhesion, proliferation, and osteogenic differentiation of bone marrow mesenchymal stem cells and the osteogenic effect in vivo

    ObjectiveTo explore the effects of concentrated growth factor (CGF) combined with mineralized collagen (MC) materials on the adhesion, proliferation, and differentiation of bone marrow mesenchymal stem cells (BMSCs) and their osteogenic effects in vivo, and to provide a theoretical basis for the combined application of CGF and MC materials in bone defect regeneration and repair.MethodsCGF was prepared from venous blood of healthy volunteers, and then CGF extracts (CGFe) were prepared. In vitro experiment: human BMSCs (hBMSCs) were divided into 4 groups. Groups A, B, and C were cultured with α-MEM medium [containing 10% fetal bovine serum (FBS) and 1% double antibody] containing 2%, 5%, and 10%CGFe, respectively; group D was cultured with α-MEM medium (containing 10%FBS and 1% double antibody) without CGFe. Scanning electron microscopy was used to observe the effect of CGFe on cell adhesion. Cell counting kit 8 (CCK-8) was used to detect the effect of CGFe on cell proliferation. After osteogenic induction, alkaline phosphatase (ALP) activity was detected and Western blot was performed to detect osteopontin (OPN) expression. In vivo experiment: Eighteen New Zealand big-eared rabbits were used to prepare circular bone defect models on the left and right mandibles, and implant CGF gel (prepared from autologous venous blood)+MC material (volume ratio 1∶1, experimental group) and simple MC material (control group), respectively. At 4, 8, and 12 weeks after operation, 6 rabbits were sacrificed respectively to obtain materials, and Micro-CT scanning was performed to observe the formation of new bone and material degradation in vivo.ResultsIn vitro experiments: Scanning electron microscopy showed that the cells of groups A, B, and C spread better on MC materials than group D, with more pseudopodia. CCK-8 method showed that different concentrations of CGFe could promote cell proliferation, and the absorbance (A) value of cells cultured for 2, 3, 5, and 7 days was in the order of group C>group B>group A>group D, the differences were significant (P<0.05). ALP activity test showed that its activity was proportional to the osteogenic induction time and CGFe concentration (P<0.05). Western blot analysis of osteogenic induction culture for 14 days showed that the relative expression of OPN protein in groups A, B, and C was significantly higher than that in group D, and the higher the CGFe concentration, the higher the relative expression of OPN protein (P<0.05). In vivo experiment: Micro-CT observation showed that the new bone formation and material degradation of the experimental group were better than those of the control group at 4, 8, and 12 weeks after operation. Quantitative detection showed that the volume of new bone volume, new bone volume fraction, trabeculae number, and trabecular thickness of the experimental group were significantly higher than those of the control group at each time point, the residual material volume, residual material volume fraction, and trabecular separation were significantly lower than those of the control group, all showing significant differences (P<0.05).ConclusionCGF can effectively promote the adhesion, proliferation, and osteogenic differentiation of BMSCs on MC materials, and 10%CGFe has the most significant effect. The combined application of CGF and MC material can significantly promote bone formation in vivo.

    Release date:2021-03-26 07:36 Export PDF Favorites Scan
  • Efficacy and safety of autologous mononuclear cells transplantation in osteonecrosis of the femoral head: a meta-analysis

    ObjectiveTo systematically review the efficacy and safety of autologous mononuclear cells transplantation in osteonecrosis of the femoral head.MethodsPubMed, EMbase and The Cochrane Library were electronically searched to collect randomized and non-randomized controlled trials on autologous mononuclear cells transplantation for osteonecrosis of the femoral head from inception to July 31th, 2020. Two reviewers independently screened literatures, extracted data and assessed risk of bias of included studies. Meta-analysis was then performed using RevMan 5.4 software.ResultsA total of 17 studies involving 645 hips in mononuclear cells group and 557 hips in cell-free group were included. The results of meta-analysis showed that compared with cell-free therapy, mononuclear cells therapy could improve hip function in term of Hairrs score (MD=8.11, 95%CI 4.36 to 11.87, P<0.000 1), Merle D`Aubigné Postel score (MD=2.23, 95%CI 0.97 to 3.49, P=0.000 5), WOMAC score (MD=−10.81, 95%CI −15.80 to −5.81, P<0.000 1), Lequesne index (MD=−2.97, 95%CI −5.42 to −0.52, P=0.02) and alleviate the pain (MD=−9.13, 95%CI −12.40 to −5.86, P<0.000 01), delay the progression of radiological staging (RR=0.55, 95%CI 0.34 to 0.89, P=0.01) and reduce the rate of total hip arthroplasty (RR=0.61, 95%CI 0.43 to 0.86, P=0.005). In terms of safety, mononuclear cell therapy did not increase the rate of complications (RR=0.77, 95%CI 0.33 to 1.83, P=0.56).ConclusionsThe current evidence shows that autologous mononuclear cells therapy is a safe and effective way for osteonecrosis of the femoral head. Due to limited quality and quantity of the included studies, more high quality studies are required to verify above conclusions.

    Release date:2021-05-25 02:52 Export PDF Favorites Scan
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