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find Author "王桂芳" 8 results
  • Dendritic CellsOverloaded Antigens Injected Intratracheally Induce Lung Inflammation

    Objective To investigate the feasibility of dendritic cells ( DCs ) as vector of immunotherapy through intratracheal injection. Methods The DCs obtained from the bone marrow of BALB/ c mice were cultured and isolated with CD11c-positive magnetic beads. Then DCs were overloaded with ovalbumin peptide 323-339 ( OVA 323-339) for 24 hours. The mice in the DC-OVA group were intratrachelly injected DCs overloaded with OVA 323-339 in dose of 2 ×106 cells per mouse. The mice in thenegative control group were intratracheally injected with DCs untreated by OVA 323-339. On the second day,all mice were challenged with 1% OVA in PBS lasting for five days. The asthma animal model established by classic method was used for the positive control. Pathologic changes in lung and cell numbers in bronchoalveolar lavage fluid ( BALF) were assayed 24 hours after challenged. Results Just like the lung tissues from the mice asthma models, the lung tissues from the mice instilled with DCs overloaded with allergen OVA 323-339 showed extensive inflammatory cells infiltration, most of which were eosinophils, neutrophils and lymphocytes. The lung tissues in the DC group showed no obvious inflammation. There were more cells in BALF in the DC-OVA group than that in the DC group. OVA-specific IgE in serum from the DC-OVA group was not significantly different from that in the mice asthma models [ ( 48. 22 ±4. 76) U/mL vs. ( 52. 75 ±4. 03) U/mL, P gt;0. 05] . Conclusion DCs overloaded antigen has the ability of transferring of antigen effectively and may be used as vectors of immunotherapy.

    Release date:2016-08-30 11:54 Export PDF Favorites Scan
  • 氧化应激失衡在慢性阻塞性肺疾病患者发病及防治中的研究进展

    慢性阻塞性肺病( COPD) 由于其患者人数多, 疾病致残率和死亡率高, 已经成为社会和患者家庭的严重经济负担,并成为一个非常重要的公共卫生问题。目前全球大约有6亿人患有COPD, 根据世界银行/ 世界卫生组织发表的研究,至2020 年COPD 将成为世界疾病经济负担的第五位, COPD将成为全球第三大致死原因[ 1] 。在我国, COPD 同样是严重危害公众健康的重要慢性呼吸系统疾病, 抽样研究发现人群的COPD总患病率为9. 4% [ 2] , 肺心病患病率为4. 4% [ 3] 。这提示我国COPD 患病率、死亡率之高以及占用大量社会资源的后果是十分惊人的。 慢性阻塞性肺疾病全球创议( Global Initiative for Chronic Obstructive Lung Disease, GOLD) 推广以来, 规范性管理COPD 的诊治, 对稳定期COPD 的治疗是以长效支气管舒张剂为主的吸入治疗, COPD 患者的生活质量提高, 急性加重次数及疾病的社会负担下降。然而, 尽管采用规范治疗, 仍不能完全阻止其急性加重的发生, 特别是对肺功能位于GOLD 分级Ⅲ、Ⅳ者来说, 由于急性加重是COPD 患者生活质量及肺功能下降的因素, 因此, 寻找更加积极的治疗措施,对于治疗及管理COPD 来说极有意义。近年来几个关于支气管舒张剂的临床研究并不能防止COPD 患者FEV1 的下降, 因此氧化应激失衡就再次进入了视线。本文综述了近年关于氧化应激失衡在COPD 中的应用及研究进展, 为抗氧化治疗在COPD 的管理中确立稳定地位提供一些基础。

    Release date:2016-09-13 04:06 Export PDF Favorites Scan
  • 病毒感染在慢性阻塞性肺疾病急性发作中的作用

    慢性阻塞性肺疾病( COPD) 是一种进行性肺功能下降的呼吸系统慢性疾病, 在全世界是第四位导致死亡的原因, 由其导致的误工、医疗等费用巨大。另外, COPD 急性发作( AECOPD) 是导致生活质量下降和肺功能减退的主要原因[ 1] 。尽管AECOPD是一个严重的临床和经济问题, 然而, 关于AECOPD的发生机制目前尚不完全清楚。目前认为感染是独立的导致AECOPD 最重要的原因, 在所有AECOPD的病因中约占70% 。关于细菌感染在AECOPD 中的地位, 由于细菌相对较易分离、有明确治疗效果等, 容易确认, 而病毒相对难于分离和检测, 治疗效果相对不佳, 且人们对其重要性缺乏认识等, 在AECOPD 中的重要性研究较少。本文综述了近年研究中发现的病毒感染在AECOPD 中作用。......

    Release date:2016-09-13 04:00 Export PDF Favorites Scan
  • Efect of bacterial infection on airway inflammation in acute exacerbation of chronic obstructive pulmonary disease patients

    0bjective To study the effect of bacterial infection on acute exacerbation of chronic obstructive pulmonary disease(AECOPD),and to compare the airway inflammation caused by different isolated bacteria.Methods A total of 159 sputum samples were collected from AECOPD patients diagnosed according to GOLD 2004 standard,in which conventional culture and identification of bacteria was conducted.The patients with purulent sputa were divided into different groups according to bacteria separated.Levels of IL-6.IL-8 and TNF-α in sputum supernatant were assayed and compared in different bacteria groups.the purulent sputum without isolated bacteria group(NG)and normal control group(NC). Results One hundred and twenty-nine strains of bacteria were isolated in 159 qualified sputa,including 26 strains of Klebsiella pneumoniae(KB),21 strains of Hemophilus influenza(Hi),17 strains of Pseudomonas aeruginosa(PA),37 strains of Haemophilus parainflb~enzae(HP)(mixed infection not included)and 28 strains of other bacteria.Among of all samples,20 were double infection of Haernophilus parainfluenzae with another bacterium.Ninety-seven purulent sputa were collected.According to bacteria isolated,these sputa were divided into five groups,named HP(24 samples),Hi(20 samples),PA(16 samples),KB(19 samples)and NG(18 samples).Contrast to NC,concentration of IL-8 and TNF-α rose in sputa from which PA,Hi,KB were isolated(Plt;0.05).The level increased much more in sputa from which PA and Hi were isolated compared with KB(Plt;0.05).Concentration of IL-6 rose in sputa of each group collected from AECOPD patients contrast to sputa collected from NC(Plt;0.05),without significant differences among all other group except for NC.Conclusions Bacterial infection plays an important role in AECOPD characterized with high level of inflammatory factors especially when PA,Hi,KB were infected bacteria.This study provides evidence for antibacterial therapy in AECOPD patients.

    Release date:2016-09-14 11:57 Export PDF Favorites Scan
  • 系统性红斑狼疮继发视网膜中央静脉阻塞1例

    Release date:2024-09-20 10:50 Export PDF Favorites Scan
  • The effects of nontypeable Hemophilus influenzae on proinflammatory cytokine expression of human lung epithelial cell line

    Objective To study the effect of nontypeable Hemophilus influenzae(NTHi) strain ATCC49247 on proinflammatory cytokines expression of human A549 lung epithelial cell line. Methods Confluent A549 cells were co-incubated with NTHi, NTHi+Erythromycin(10 mg/L), NTHi+Gentamicin(100 mg/L), and NTHi+Dexamethasone(100 μmol/L),and nuclear factor kappa B(NF-κB) inhibitor primed cells were co-incubated with NTHi for 24 h. Then levels of interleukin-8(IL-8) and tumor necrosis factor-α(TNF-α) in the supernatant was assayed by enzyme-linked immunosorbent assay(ELISA) and the expression of intercellular adhesion molecule-1(ICAM-1) in cells was detected by immunohistochemistry staining. Results A549 cells were transformed and died after co-intubated with NTHi for 24 h. NTHi induced A549 cells to release significantly greater amounts of IL-8, which was inhibited by NF-κB inhibitor pyrrolidine dithiocarbamate(PDTC). Incubating of A549 cells with NTHi significantly induced release of IL-8 and the expression of ICAM-1, which was blocked by erythromycin and dexamethasone and not by gentamicin. TNF-α was not detected in all circumstances. Conclusions NTHi can increase significantly the release and expression of proinflammatory cytokines through NF-κB pathway. Antibacterial drug erythromycin also has anti-inflammatory effect.

    Release date:2016-09-14 11:56 Export PDF Favorites Scan
  • Acute Bronchodilator Effect of Long Term Bronchodilator Tiotropium on COPD Patients

    Objective To investigate whether long term bronchodilator tiotropium has an acute bronchodilator effect on COPD patients. Methods 46 patients with stable COPD were enrolled in the study.Lung function test was performed before and at 10 min, 20 min, 1 h after inhaling tiotropium. FEV1 , FVC,FEV1/FVC, PEF25% -75% were measured by ambulatory spirometer. The patients were followed up after 1 month.Results The mean FEV1 was ( 1. 110 ±0. 34) L before inhaling tiotropiumand ( 1. 172 ±0. 359) L, ( 1. 221 ±0. 391) L, ( 1. 225 ±0. 392) L at 10 min,20 min, 1 h after inhaling tiotropium, respectively. FEV1 at 1 h after inhaling tiotropiumsignificantly increased compared with that before inhaling tiotropium. FVC also increased and reached highest at 1 h after inhaling tiotropium. PEF25%-75% at 1 h after inhaling tiotropium increased, but there was no significance difference compared with that before inhaling tiotropium. Mean FEV1 was 1. 287 Lafter 1 month, with significant difference compared with baseline. Conclusion Tiotropium can release the symptoms and improve compliance of COPD patients for its acute bronchodilator effect on COPD patients.

    Release date:2016-09-13 03:50 Export PDF Favorites Scan
  • 子宫内膜癌相关性视网膜病变一例

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