Objective To study the growth characteristics of umbil ical cord MSCs (UCMSCs) in vitro and its effect on the nerve regeneration after spinal cord injury (SCI). Methods UCMSCs isolated from pregnant rats umbil ical cord were cultured and purified in vitro. Sixty female Wistar rats weighing (300 ± 10) g were randomized into three groups (n=20per group). UCMSCs group (group A) in which UCMSCs suspension injection was conducted; DMEM control group (groupB) in which 10% DMEM injection was conducted; sham group (group C) in which the animal received laminectomy only.Establ ish acute SCI model (T10) by Impactor model-II device in group A and group B. The recovery of the lower extremity was observed using BBB locomotor scoring system, neurofilament 200 (NF-200) immunofluorescence staining was performed to detect the neural regeneration, and then the corticospinal tract (CST) was observed using the biotinylated dextran amine (BDA) tracing. Results Cultured UCMSCs were spindle-shaped fibrocyte-l ike adherent growth, swirl ing or parallelly. The USMSCs expressed CD29, but not CD31, CD45, and HLA-DR. The BBB score was higher in group A than group B 4, 5, and 6 weeks after operation, and there was a significant difference between two groups (P lt; 0.05). The BBB scores at different time points were significantly lower in groups A and B than that in group C (P lt; 0.05). UCMSCs was proved to survive and assemble around the injured place by frozen section of the cords 6 weeks after injury. NF-200 positive response area in groups A, B, and C was (11 943 ± 856), (7 986 ± 627), and (13 117 ± 945) pixels, respectively, suggesting there was a significant difference between groups A, C and group B (P lt; 0.05), and no significant difference was evident between group A and group C (P gt; 0.05). BDA anterograde tracing 10 weeks after operation demonstrated that more regenerated nerve fibers went through injured area in group A, but just quite few nerve fibers in group B went through the injuried cavity. The ratios of regenerative axons amount to T5 axons in group A and group B were smaller than that of group C (P lt; 0.05). Conclusion UCMSCs can prol iferate rapidly in vitro, survive and differentiate to neurons after being grafted into injured spinal cord. The transplantation of UCMSCs is effective in promoting functional recovery and axonal regeneration after SCI.
Objective To evaluate the therapeutic effect and safety of Bupropion hydrochloride sustained-release tablets in the treatment of depression. Methods A total of 48 patients meeting the diagnostic criteria of depression of CCMD-3 were randomly treated with Bupropion hydrochloride sustained-release tablets or Fluoxetine tablets for 42 days. Hamilton depression rating scale, Hamilton anxiety rating scale, clinical global impression and treatment emergent symptom scale were used to evaluate the therapeutic effect. Blood routine test, urine routine test and electrocardiogram were examined before and after the treatment. Results The effective rate of Bupropion hydrochloride sustained-release tablets [83% (20/ 24) ] was higher than that of Fluoxetine tablets [63% (15/ 24)], with a P value of 0.104. The incidence of adverse reactions was 46% (11/24) in both groups. Conclusion The therapeutic effect of Bupropion hydrochloride sustained-release tablets on depression is similar to that of Fluoxetine tablets, with mild adverse reactions to both treatments.
For observation of the change of transforming growth factor-beta 1 (TGF-beta 1) gene expression in the process of skin wound healing, the following experiments were performed. Sixteen Wistar rats were chosen. At each side of the rat’s back, a 1 cm x 1.5 cm middle-thick skin wound was made. After 3, 6, 9 and 12 days, the specimens were taken from the wounds. For each specimen, half of it was used for RNA extraction, and underwent dot blotting; and the other half was frozen immediately and underwent in situ hybridization. The probes were dig-labeled PDGF-BB cDNA probe and TGF-beta 1 probe. The results showed that TGF-beta 1 gene was expressed mainly in fibroblast, epithelial cell and capillary endothelial cell. The peak of TGF-beta 1 mRNA content was in the 6th day postoperatively. After that, the content of TGF-beta 1 decreased to normal. It was suggested that TGF-beta 1 gene expression was in close relation with healing process. TGF-beta 1 may play an important regulatory role in the skin wound healing.
目的 比较中高度散光患者配戴框架眼镜和透气性硬性接触镜（RGPCL）的主客观视觉质量。 方法 选取2008年6月－2011年5月中高度角膜散光20例共40只眼进行角膜地形图、综合验光仪验光等检查后，选择合适试戴片作配适评估并定制RGPCL。要求患者戴镜后1周、1个月、3个月和6个月复查，记录矫正视力、镜片配适、眼部情况，并在第4次复查时进行对比敏感度视力检查及主观问卷调查。 结果 RGPCL矫正视力优于框架眼镜，但两者对比敏感度视力在各空间频率均无明显差异。主观评分时，远视力两者无明显差别；中距离视力框架眼镜评分（3.825 ± 0.245）分，RGPCL评分（3.525 ± 0.302）分，差异有统计学意义（t=5.339，P=0.000）；近距离视力框架眼镜评分（3.795 ± 0.233）分，RGPCL评分（3.690 ± 0.194）分，差异有统计学意义（t=3.462，P=0.030）。有45%患者选择RGPCL为主要配戴方式；40%患者选择RGP CL和框架眼镜交替使用的方式；10%患者选择仅在有特殊社交需求时使用RGPCL；另5%患者放弃使用RGPCL。 结论 RGPCL和框架眼镜矫正中高度角膜散光均能取得较满意效果，在中近距离精细作业时框架眼镜矫正视力更为稳定清晰。但由于RGPCL在成像质量和外观上的优势，多数患者仍愿意坚持配戴RGPCL。
ObjectiveTo study the results of high tibia osteotomy (HTO) combined with posterior cruciate ligament (PCL) reconstruction for osteoarthritis (OA) of the medial compartment with PCL injury. MethodsBetween March 2008 and June 2014, 11 patients with OA of the medial compartment and PCL injury underwent HTO combined with PCL reconstruction. There were 5 males and 6 females, aged 43-55 years (mean, 50.3 years). All patients had a trauma history, and the duration of injury was 3-5 years (mean, 3.7 years). At preoperation, Hospital for special surgery (HSS) score was 54.73±8.60, Lysholm score was 56.91±4.51, KT-1000 test was (5.71±1.13) mm, and knee range of motion (ROM) was (125.21±4.77)°. The preoperative femoral tibia angle (FTA) and posterior slope angle (PSA) of the tibia plateau were (184.82±2.40)° and (7.18±1.17)° on the X-ray film. ResultsIncisional fat liquefaction occurred in 1 case, and wound healed after dressing change; primary healing of wound was obtained in the other cases. All 11 cases were followed up 12-28 months (mean, 17 months). Bone union was observed at osteotomy site within 6 months, without delayed union or nonunion. After operation, genu varus deformity was corrected with different degrees; the stability of knees was improved in all patients; and the pain of medial knee was released significantly. At 12 months after operation, the FTA was significantly reduced to (176.64±1.96)°; at last follow-up, the HSS score was significantly increased to 88.27±4.76, KT- 1000 test was significantly reduced to (3.18±0.87) mm, and Lyholm score was significantly increased to 86.45±2.34, all showing significant differences when compared with preoperative ones (P<0.05). At last follow-up, the knee ROM was (124.63±2.98)° and the PSA was (7.91±1.30)°, showing no significant difference when compared with preoperative ones (P>0.05). ConclusionThe PSA will not be changed when a combination of HTO and PCL reconstruction is used to treat OA of the medial compartment with PCL injury if the right osteotomy site and reasonable bone graft are selected. The short-term effectiveness is good because of good recovery of the lower extremity force line and knee stability, but the long-term effectiveness remains to be further followed up.