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find Keyword "生物传感器" 3 results
  • 感染性疾病实验室诊断技术及其研究进展

    目前全球感染性疾病现状严峻,应用于感染性疾病诊断的实验室技术种类繁多,为了解感染性疾病实验室诊断技术及其最新进展,帮助临床工作者早期、快速、准确地诊断感染性疾病,降低感染性疾病暴发的危险性,及时启动感染性疾病的正确治疗等,现从病原学检测、免疫学技术、分子生物学技术、生物传感器、流式细胞术这几个方面对感染性疾病诊断技术进行综述。

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  • Study of TGF-β/Smad3 Signal Pathway Using the Technology of Flurorescence Resonance Energy Transfer

    The transforming growth factor-β1 (TGF-β1)/Smad3 signal pathway is related to mutiple physiological and pathological generation mechanism of human being. Up to date, however, the spacial and time information on the phosphorylated Smad3 is still unclear. In this study, the process of Smad3 phosphorylation was observed under the physiological state in the living cells. Firstly, the ECFP-Smad3-Citrine (Smad3 biosensor) fusion protein expression vector was constructed and identified. Then the Smad3 biosensor was transfected into 293T cells. The transfection efficiency and the expressions of fusion proteins were observed in 24 hours. Thirdly, Smad3 biosensor flurorescence resonance energy transfer (FRET) was observed with the inversion fluorescence microscope and measured by the MetaFlour FRET 4.6 software. Smad3 biosensor transfection efficiency was nearly 40% and the fusion protein was seen under the fluorescence microscope. The FRET ratio of Smad3 biosensor in living 293T cells was decreased after 10 minutes incubation with the ligand of TGF-β1. The period of decreasing CFP and enhancing Citrine signals was about 300 seconds. With the technology of FRET, the TGF-β1/Smad3 signal pathway could be real time monitored dynamically under the physiological condition in living cells.

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  • The application of silicon nanowires field-effect transistor biosensor in medicine

    Ultra-sensitive and quantitative analysis of proteins, nucleic acid, virus and other biochemical species are critical technologies for effective dianosis of disease, as well as medical studies. Silicon nanowires field-effect transistor (SiNWs-FET) biosensor is one of the most promising powerful platforms for label-free, real-time, ultra-sensitive detection of analyte. Here, the working principle of SiNWs-FET biosensor and the applications of SiNWs-FET biosensors in medicine were introduced. Moreover, the methods for enhancing the sensitivity of SiNWs-FET biosensor were discussed. Lastly, the prospecting of SiNWs-FET biosensor was presented.

    Release date:2017-10-23 02:15 Export PDF Favorites Scan
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