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find Keyword "相关蛋白" 51 results
  • Protective Effect and Regulation Mechanism of Oxaloacetate on Myocardial Ischemia Reperfusion Injury in Rats

    ObjectiveTo investigate the protective effect and the regulation mechanism of oxaloacetate (OAA) on myocardial ischemia reperfusion injury in rats. MethodsSixty rats, weight ranged from 200 to 250 grams, were randomly divided into 6 groups:a negative control group, a sham operation control group, a model control group, an OAA pretreatment myocardial ischemia-reperfusion model group (three subgroups:15 mg/kg, 60 mg/kg, 240 mg/kg). We established the model of myocardial ischemia reperfusion of rats and recorded the internal pressure of left ventricle (LVSP), the maximal rate of left ventricular pressure change (±dp/dtmax) and left ventricular end diastolic pressure (LVEDP). We restored reperfusion 180 minutes after ligating the left anterior descending coronary artery 30 minutes and determinated cardiac troponin Ⅰ (cTn-I), lactate dehydrogenase (LDH), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px). We took out heart tissues, stained it and calculated the infarcted size. We used the Western blot to detect the expression of NF-E2 related factor 2 (Nrf2), Kelch-like ECH-associated protein-1 (Keap1) and heme oxygenase-1 (HO-1). ResultsCompared with the sham operation group, heart function indexes in the negative control group had no significant difference (P>0.05). But in the model control group there was a decrease (P<0.05) And the serum levels of LDH, cTn-I, and myocardial infarcted size were significantly increased (P<0.01). Compared with the model control group, heart function indexes in the OAA pretreatment groups improved, the serum LDH, cTn-I activity, and infarct size decreased (P<0.05), SOD and GSH-Px activity increased (P<0.05). And these results were statistically different (P<0.01) in the high dose OAA pretreatment groups. Compared with the model control group, the expression of Keap1 in the OAA pretreatment group was down-regulated (P<0.001) while total Nrf2, nucleus Nrf2 and its downstream HO-1 was up-regulated (P<0.001), which suggested that OAA enhanced antioxidant capacity by (at least in part) Keap1-Nrf2 pathway, resulting in reducing myocardial damage and protecting myocardium after acute myocardial ischemia reperfusion injury. ConclusionOxaloacetate can provide protective effects on myocardial ischemia reperfusion injury through down-regulating the expression of Keap1 and up-regulating the expression of Nrf2 and its downstream peroxiredoxins to improve antioxidant capacity.

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  • Construction of the Recombinant Adenovirus Carrying Antisense Multidrug ResistanceAssociated Protein and the Study of Its Application

    ObjectiveTo construct the recombinant adenovirus vector carrying antisense multidrug resistanceassociated protein (MRP) and transfect the human drugresistant hepatocellular carcinoma cell line(SMMC7721/ADM). MethodsThe fragment of MRP gene encoding 5′region was cloned reversely into the shuttle plasmid pAdTrackCMV, with the resultant plasmid and the backbone plasmid pAdEasy1,the homologous recombination took place in the bacteria and the recombinant adenoviral plasmid was generated. The adenoviruses were packaged and amplified in 293 cells. Then the cell line of SMMC7721/ADM was transfected with the resultant adenoviruses.ResultsThe recombinant adenovirus vector carrying antisense MRP was constructed successfully. The viral titer was 2.5×109 efu/ml, and more than 90% SMMC7721/ADM cells could be transfected when the multiplicity of infection(MOI) was 100. ConclusionThe recombinant adenovirus vector constructed by us could introduce the antisense MRP into the human drugresistant hepatocellular cell line effectively, which would provide experimental basis for the mechanisms and reversal methods of the multidrug resistance in human hepatocellular carcinoma.

    Release date:2016-08-28 04:48 Export PDF Favorites Scan
  • THE ROLE OF MULTIDRUG RESISTANCE-ASSOCIATED PROTEIN IN THE FORMATION OF ADRIAMYCININDUCED MULTIDRUG RESISTANCE TO HEPATOCELLULAR CANCER CELL SMMC-7721 IN HUMAN HEPATOCELLULAR CARCINOMA

    Objective To dynamically study the formation of multidrug resistance(MDR) of human hepatocellular carcinoma cell SMMC-7721 induced by Adriamycin (ADM) and the role of multidrug resistance-associated protein(MRP) in its mechanisms.Methods Hepatocellular carcinoma cell SMMC-7721 was cultured in RPMI-1640 medium containing ADM with progressively increased concentration or directly cultured in medium containing different concentrations of ADM. Resistant index of drug-resistant variants of SMMC-7721 cell was determined by drawing cell dosage-reaction curves.Levels of MRP mRNA expression were detected by reverse transcription-polymerase chain reaction(RTPCR). Intracellular rubidomycin(DNR) concentration was examined by flow cytometry(FCM).Results With progressive increasing of ADM concentration in medium resistant index and levels of MRP mRNA expression were correspondingly increased but intracellular DNR concentration was markly reduced. When parental cells were directly cultured in medium containing different concentrations of ADM, the higher the ADM concentration, the higher the level of MRP mRNA expression, but intracellular DNR concentration was kept at the similar high level and most cells died. Conclusion ADM may progressively induce SMMC-7721 cell resistant to multiple chemotherapeutic drugs with reduced intracellular DNR accumulation associated with the overexpression of MRP gene.

    Release date:2016-08-28 05:29 Export PDF Favorites Scan
  • The Costimulatory Pathway B7CD28/CTLA4 and the Islets of Langerhans Transplantation

    ObjectiveTo investigate the significant effect of costimulatory pathway B7CD28/CTLA4 on the islets of Langerhans transplantation. MethodsThe literatures were reviewed to summarize the molecular structure and functions of the pathway and the related animal experiments.ResultsThe costimulatory pathway B7CD28/CTLA4 was one of the signaling pathways of T cells activation and proliferation. If the costimulatory signals were absent, Tlymphocyte would be induced to the clonalanegy. Through blocking the costimulatory pathway mediated by CD28, CTLA4Ig prolonged to the islets of Langerhans survival in recipients. ConclusionBy the studies of the costimulatory pathway, it is helpful to understand the immune mechanism of the survival of islet grafts.

    Release date:2016-08-28 05:12 Export PDF Favorites Scan
  • A QUANTITATIVE STUDY ON NUCLEUS PLASMA RATIO AgNORs COUNTS OF CHOLANGIOCARCINOMA AND ATYPICAL EPITHELIAL HYPERPLASIA RELATED TO HEPATOLITHIASIS

    Nucleus plasma ratio was measured and silver-binding nucleolar organizer (AgNORs) were counted in 31 cases of cholangiocarinoma (11 cases were well-differentiated, 10 case moderately differentiated and 10 cases poorly differentiated) and in 17 cases of atypical epithelial hyperplasia related to hepatolithiasis (9 cases were simple hyperplasia, 8 cases atypical epithelial hyperplasia) by AgNORs techique and image analysis.The results showed that mucleus plasma ratio and AgNORs counts increased significantly from well-differentiated to poorly differentiated cholangiocarcinoma (P<0.01). No statistically significant differance was shown between nucleus plasma ratio of atypical hyperplasia and well-differentiated cholangiocarinoma.The data imply that chronic proliferative cholngitis in the presence of hepatolithiasis can progress to atypical epithelial hyperplasia which may be an important precursor of cholangiocarcinoma.

    Release date:2016-08-29 03:19 Export PDF Favorites Scan
  • The Expression of SIGIRR in Normal Human Lung Tissues and its Changes in the Acutely Injured Alveolar Epithelial Cells Induced by Lipopolysaccharide

    Objective To detect the expression of single immunoglobin IL-1 receptor related protein ( SIGIRR) in normal human lung tissues, and study its changes in alveolar epithelial cell acutely injured by lipopolysaccharide ( LPS) . Methods Twenty samples of human normal lung tissue were collected during the lobectomies. The expression of SIGIRR was detected by immunohistochemistry, western blot and RT-PCR. The human type II alveolar epithelial cell acute injury model was established by stimulating A549 cells with LPS of a final concentration of 10 μg/mL. The cells were collected at 0, 3, 6, 12, and 24 hours after the stimulation. The changes of SIGIRR expression at the same time points were observed by western blot. The expression vector containing full-length SIGIRR cDNA was transfected transiently into A549 cells to induce SIGIRR overexpression. MTT assay was performed to measure the injury of A549 cells caused by LPS. Results The immunohistochemistry, western blot and RT-PCR showed that there was a high expression of SIGIRR in normal human lung tissues. The expression of SIGIRR was located in alveolar epithelial cells by immunohistochemistry. The expression of SIGIRR at 3, 6, and 12 hours was down-regulated after LPSstimulation and raised again at 24 hours to the baseline. MTT assay showed that SIGIRR overexpression substantially reduced the growth inhibition ratio of A549 cells after LPS stimulation. Conclusions Expression of SIGIRR in normal human lung tissues was confirmed by different detection methods. SIGIRR alleviates the injury of alveolar epithelial cells caused by LPS, implying SIGIRR might be involved in the regulationof acute lung injury mediated by LPS.

    Release date:2016-09-14 11:23 Export PDF Favorites Scan
  • Effects of Single Immunoglobin IL-1 Receptor Related Protein on Inflammation Induced by High Mobility Group Box 1 in A549 Cells

    Objective To identify the effects of single immunoglobin IL-1 receptor related protein (SIGIRR) on inflammation induced by high mobility group box 1 (HMGB1) in A549 derived from human alveolar epithelial cells. Methods Eukaryotic expression vectors pCDNA3.1(+) constructed with SIGIRR cDNA were transiently transfected into A549 cells,in which SIGIRR was forced to be over-expressed. Western blot and RT-PCR were applied to detect the expression level of SIGIRR after transfection. After the stimulation by HMGB1,the transcriptional activity of NF-κB in A549 cells was detected by dual-luciferase reporter assay system,and the protein levels of inflammatory cytokine TNF-α and IL-1β were measured by ELISA. Results The expression level of SIGIRR increased significantly in A549 cells transfected with SIGIRR vectors. The transcriptional activity of NF-κB was enhanced obviously after HMGB1 treatment in A549 cells by dual-luciferase reporter assay system,while the transfection of SIGIRR vectors decreased the activity. The protein levels of TNF-α and IL-1β were down-regulated in A549 cells over-expressing SIGIRR after HMGB1 stimulation compared with the non-transfected cells. Conclusions Up-regulated SIGIRR expression can inhibit HMGB1-induced proinlammatory cytokine release in A549 cells such as TNF-α and IL-1β. The transcriptional activity of NF-κB is dampened by SIGIRR transfection,implying that the anti-inflammatory effects of SIGIRR may be involved in the regulation of NF-κB.

    Release date:2016-08-30 11:58 Export PDF Favorites Scan
  • Effect of Secreted Frizzled-related Protein 2 and Wnt Signaling Pathway in Myocardial Regeneration Treatment

    Abstract: Stem cell paracrine has been considered as the main mechanism to promote infarcted myocardium regeneration and repair of damaged cardiomyocytes. With further research, secreted frizzled-related protein 2 (Sfrp 2) and stem cell paracrine are closely linked to each other. Sfrp 2 can competitively bind to the specific receptor Fz in Wnt signaling pathway, inhibit Wnt signaling pathway to regulates apoptosis, differentiation, and other life processes of stem cells, and therefore becomes a research hotspot in recent years. This review focuses on the mechanism of Sfrp 2/Wnt signal way in stem cell therapy for myocardial infarction.

    Release date:2016-08-30 05:50 Export PDF Favorites Scan
  • 心肺转流术中血清对培养大鼠肺泡Ⅱ型上皮细胞SP-A的影响

    目的 研究肺表面活性物质相关蛋白A(SP-A)在心肺转流术(CPB)中的变化及机制,并观察己酮可可碱(PTX)对SP-A的保护作用。方法 改良原代培养大鼠肺泡Ⅱ型上皮细胞(AT-Ⅱ),将AT-Ⅱ与CPB中血清共同孵育,并设置PTX用药组,观察AT-Ⅱ的形态学改变及培养液中丙二醛(MDA)的变化,通过免疫组织化学、原位杂交方法检测SP-A和SP-A信使核糖核酸(SP-A mRNA)的表达。结果 损伤实验组AT-Ⅱ形态呈损伤性改变,培养液中丙二醛升高,细胞脱落率上升,成活率下降,SP-A表达在蛋白转录和翻译水平均明显降低,PTX组SP-A水平稍高。结论 CPB术后血清能直接损伤AT-Ⅱ并影响SP-A翻译和转录,这可能是术后肺表面活性物质质和量异常的重要原因,PTX能有效阻止CPB术后血清对SP-A的抑制作用。

    Release date:2016-08-30 06:33 Export PDF Favorites Scan
  • 多药耐药基因和多药耐药相关蛋白基因在食管、贲门癌中的表达

    目的 探讨多药耐药基因(MDR1)和多药耐药相关蛋白基因(MRP)在食管癌、贲门癌中表达的临床意义.方法 采用逆转录-多聚酶链反应(RT-PCR),对29例食管癌、贲门癌癌组织及癌旁组织中MDR1和MRP的表达进行检测.结果 癌组织中MDR1阳性率为65.5%,高于癌旁组织中MDR1的阳性率,为37.9%(P<0.05),癌组织MDR1信使核糖核酸(mRNA)表达水平也显著高于癌旁组织(P<0.01);癌组织的MRP阳性率为48.3%,高于癌旁组织(27.6%),但无差异(P>0.05),而癌组织MRP mRNA表达水平与癌旁组织比较则有差异(P<0.05);中、低分化肿瘤的MDR1和MRP表达阳性率增高,两基因的mRNA表达水平显著高于高分化肿瘤(P<0.05).结论 食管、贲门癌具有内源性多药耐药性;MDR1和MRP表达与食管、贲门癌的组织学类型及TNM分期无关,但可反映其肿瘤组织分化不良的生物学特征.

    Release date:2016-08-30 06:35 Export PDF Favorites Scan
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