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find Keyword "石蜡切片" 2 results
  • 兔肌腱组织石蜡切片的制备

    目的 探索在动物实验条件下一种兔肌腱的石蜡制片方法。 方法 经手术分别获取新西兰大白兔1 cm长左右侧后爪2~3趾的趾深屈肌腱。左侧后肢为正常组,切取后立即投入改良的4%多聚甲醛中室温固定1 d;右侧后肢为实验组,暴露后爪趾深屈肌腱后,注射10 μl腺病毒载体,术后第3天于注射点切取,并立即投入改良4%多聚甲醛,室温固定1 d。流水冲洗过夜后用酒精正丁醇混合液进行梯度脱水,二甲苯透明,浸蜡、包埋并切片。烤片后常规脱蜡至水,HE染色。 结果 光镜下,正常组肌腱的胶原纤维束平行而整齐紧密地排列,束间有沿其长轴成行平行排列的肌腱细胞,肌腱外膜可见成排的腱细胞。实验组肌腱外膜有炎性反应性增生,组织结构完整,染色鲜艳。 结论 为肌腱形态学方面的实验研究提供了一条新的便捷途径。

    Release date:2016-09-01 09:22 Export PDF Favorites Scan
  • Analysis of intraoperative frozen section diagnosis of 1 263 pulmonary nodules

    ObjectiveTo explore the key points and difficulties of intraoperative frozen section diagnosis of pulmonary diseases. MethodsThe intraoperative frozen section and postoperative paraffin section results of pulmonary nodule patients in Beijing Chaoyang Hospital, Capital Medical University from January 2021 to January 2022 were collected. The main causes of misdiagnosis in frozen section diagnosis were analyzed, and the main points of diagnosis and differential diagnosis were summarized. ResultsAccording to the inclusion criteria, a total of 1 263 frozen section diagnosis results of 1 178 patients were included in the study, including 475 males and 703 females, with an average age of 58.7 (23-86) years. In 1 263 frozen section diagnosis results, the correct diagnosis rate was 95.65%, and the misdiagnosis rate was 4.35%. There were 55 misdiagnoses, including 18 (3.44%) invasive adenocarcinoma, 17 (5.82%) adenocarcinoma in situ, 7 (35.00%) mucinous adenocarcinoma, 4 (2.09%) minimally invasive adenocarcinoma, 3 (100.00%) IgG4 related diseases, 2 (66.67%) mucinous adenocarcinoma in situ, 1 (16.67%) atypical adenomatous hyperplasia, 1 (14.29%) sclerosing pulmonary cell tumor, 1 (33.33%) bronchiolar adenoma, and 1 (100.00%) papillary adenoma. ConclusionIntraoperative frozen section diagnosis still has its limitations. Clinicians need to make a comprehensive judgment based on imaging examination and clinical experience.

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