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find Author "章锐" 3 results
  • Terlipressin Combined with Somatostatin in Treatment of Hemorrhage of Esophagogastric Varices Due to Portal Hypertension in Liver Cirrhosis

    目的观察特利加压素联合生长抑素治疗肝硬变门静脉高压食管胃底曲张静脉破裂出血的效果。 方法我院2008年3月至2012年8月期间收治的47例肝硬变门静脉高压食管胃底曲张静脉破裂出血患者入院时采用掷硬币法被随机分为特利加压素+生长抑素组(n=24)和生长抑素组(n=23),分别给予联合用药或单独使用生长抑素,计算24 h止血率、总止血率、1周再出血率、1周死亡率,观察不良反应发生、尿量及腹水量情况。 结果特利加压素+生长抑素组24 h止血率、总止血率和不良反应发生率明显高于生长抑素组(P<0.05),1周再出血率明显低于生长抑素组(P<0.05),2组1周死亡率比较差异无统计学意义(P>0.05),特利加压素+生长抑素组尿量明显多于生长抑素组(P<0.05),盆腔腹水量明显少于生长抑素组(P<0.05)。 结论特利加压素联合生长抑素用于肝硬变门静脉高压食管胃底曲张静脉破裂出血止血效果确切,可以明显降低死亡率;虽然其不良反应发生率略高,但均可以控制。

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  • Osteoglycin inhibits proliferation of Luminal breast cancer cells via up-regulating expression of estrogen receptor

    ObjectiveTo investigate whether Osteoglycin (OGN) gene inhibits the proliferation of Luminal breast cancer cells by up-regulating the expression of estrogen receptor (ER). Methods① Ualcan online database was used to analyze the expression of OGN in the breast cancer, and Kaplan-Meier Plotter was used to analyze the effect of OGN on the prognosis of patients with breast cancer. The median OGN mRNA expression level was taken as the cut-off point for high or low OGN expression. ② The expression of OGN mRNA in the Luminal breast cancer tissue of clinical case was examined using real time quantitative PCR (qRT-PCR). ③ Up-regulation of OGN expression in the Luminal breast cancer cell lines MCF-7 and T47D cells by transfection of overexpressing OGN plasmid, the expressions of OGN and ER were detected by qRT-PCR and Western blot, respectively. CCK8 assay and colony formation assay were applied to detect the cell proliferation and colony formation of Luminal breast cancer cells. ④ siRNA transfection was used to interfere with the expression of ER (ESR1) of breast cancer cells in the overexpressing OGN of breast cancer cells, then the CCK8 assay was used to detect the proliferation ability of the breast cancer cell lines after down-regulating the expression of ER in the overexpressing OGN patients. Results① The results of Ualcan online database showed that the expression of OGN mRNA in the breast cancer tissues of different types of breast cancer was lower than that in the normal breast tissues (P<0.001), and which was highest in the Luminal breast cancer tissues (P<0.001). The Kaplan-Meier Plotter prognosis analysis showed that in all breast cancer or Luminal breast cancer patients, the prognosis of patients with high OGN expression was better than those with low OGN expression (P=0.000 14, P=0.001 80). ② The OGN mRNA expression was decreased in the 30 Luminal breast cancer samples as compared with the corresponding adjacent normal breast tissues (t=4.774, P=0.000 019). ③ The expressions of OGN and ER in the MCF-7 and T47D cells were up-regulated after transfection of overexpressing OGN plasmid (P=0.000 002, P=0.000 001). The cell proliferation was inhibited (P<0.05) and the number of cell clones was decreased significantly (P<0.05). ④ After transient transfection of siRNA interfered with breast cancer cell lines of overexpressing OGN, ER mRNA level decreased (P<0.05), and cell proliferation ability increased significantly (P<0.05). Conclusion OGN could exert a tumor suppressor effect in Luminal breast cancer by mediating expression of ER.

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  • 利用 TCGA 数据库构建男性乳腺癌的lncRNA-miRNA-mRNA ceRNA 网络

    目的通过构建男性乳腺癌的竞争性内源 RNA(ceRNA)调控网络,探讨其发病机制。方法从 TCGA 数据库(The Cancer Genome Atlas Database)中下载男性乳腺癌的长链非编码 RNA(lncRNA)、微小 RNA(miRNA)和 mRNA 的表达谱数据,通过 R 软件的 limma 数据包分析男性乳腺癌差异表达的 lncRNA、miRNA 和 mRNA;分析三者之间的靶向调控关系,构建 ceRNA 网络,并用 Cytoscape 软件可视化,并将差异表达基因进行 GO 富集和 KEGG 通路分析。结果男性乳腺癌中差异表达的 lncRNA 为 275 种,差异表达的 miRNA 为 33 种,差异表达的 mRNA 为 1 675 种。本研究成功构建了男性乳腺癌的 lncRNA-miRNA-mRNA ceRNA 网络后,GO 富集分析显示,差异表达 mRNA 参与细胞增殖的负调控、转录的负调控、细胞蛋白质代谢过程的调控、细胞迁移的调控、转移酶活性的正调控、间充质细胞增殖的正调控等。KEGG 通路分析显示,差异表达 mRNA 参与癌症的途径、白细胞跨内皮迁移、丝裂原活化蛋白激酶(MAPK)和神经营养蛋白信号通路。结论本研究基于 TCGA 数据库成功构建了男性乳腺癌的 ceRNA 网络。

    Release date:2021-04-25 05:33 Export PDF Favorites Scan
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