ObjectiveTo explore apoptosis of acinar cells during pancreatic allograft rejection in rats.MethodsGroups of Wistar rats underwent heterotopic pancreaticoduodenal transplantation from syngenic Wistar of allogenic SD rats. The grafts were harvested on postoperative day 3, 5 and 7. All graft samples were subjected to histological examination and apoptotic cells of graft acinar cells using in situ terminal deoxynucleotidy1 transferasemediated dUTP nickend labeling (TUNEL). Histopathological rejection score and apoptotic index (AI) were analyzed. ResultsThe incidence of apoptotic cells was increased steadily over time in allografts, in contrast with syngenic grafts. The apoptotic cells in allografts were mainly acinar cells and few infiltrating lymphocytes. A significant correlation between apoptotic index and histopathological rejection score was noted.ConclusionTUNEL can display apoptosis of single cell in situ. Apoptosis is an important mechanism of tissue injury in acute pancreatic allograft rejection in rats. Acinar cell apoptosis can be used as a valuble index to estimate the injury of grafts and to monitor the acute rejection.
The specimens were obtained from 7 fibrous capsules two years after mammoplasty with silicone prosthesis, and were examined by electron spin resonance (ESR) andmicroscope. The result showed that a marked quantities of oxygen free radical existed in the capsules and that the main pathological change was the infiltration of massive inflammatory cells and deposition of collagen. It was suggested that the fibrous capsule formation was concerned with the oxygen free radical. The origin of the oxygen free radical in mammoplasty with slicone was also discussed.
目的 分析眼表面肿块的发病情况及组织病理学特点。 方法 对2004年1月-2008年12月收治并经病理学证实的326例眼表面肿块患者的年龄、性别、眼别、肿块发生部位、肿块性质及病理类型进行回顾性分析。 结果 326例眼表面肿块中，良性肿块264例（81.0%），恶性肿块62例（19.0%）。良性肿块中，前5位分别为色素痣67例（25.4）%，迷芽瘤63例（23.9）%，乳头状瘤39例（占14.8）%，结膜囊肿25例（9.5）%，炎性肉芽肿20例（7.6）%。恶性肿块中，前4位分别为鳞状细胞癌25例（40.3）%，淋巴瘤13例（21.0）%，恶性黑色素瘤12例（19.4）%，原位癌10例（16.1）%。 结论 眼球表面的肿块有共同的组织细胞起源，肿块的亚型表现出不同的组织结构、良恶性和好发部位；而同部位的良性、交界性和恶性病变的衍变发展，从某种程度上体现了一个疾病的不同发展阶段，三者间的鉴别和明确的病理诊断能为临床选择手术时机及手术方式提供依据。
Lung ground glass opacity (GGO), which is associated with the pathology of the lung adenocarcinoma, is drawing more and more attention with the increased detection rate. However, it is still in the research stage for the imaging interpretation of GGO lesions. In this paper, we reviewed and analyzed the new classification of lung adenocarcinoma corresponding to the interpretation of GGO imaging feature, which emphasizes on how to determine the GGO lesions comprehensively and quantitative determination of the invasive extent of GGO.
Pulmonary carcinoid (PC) is a rare low-grade malignant neuroendocrine tumor. Unlike other neuroendocrine tumors of the digestive tract, PC seldom causes hormone-related neuroendocrine symptoms, and the diagnosis mainly depends on the biopsy of the lesion. Besides, the immunohistochemical staining technique is an important supplement and a grading standard for pathological diagnosis. Surgery is the first choice for the treatment of early PC. However, the therapy of advanced PC which is the main problem perplexing most of clinicians is controversial and still facing severe challenges. Therefore, we will discuss the current progress of the diagnosis and therapy of advanced PC.
Polypoidal choroidal vasculopathy (PCV) is originally defined as a separate disease, but with the development of imaging techniques, it has now been included in the spectrum of neovascularization. In the Asian population, the prevalence of PCV is high, and with the deepening of clinical studies, the pathological characteristics, pathogenesis and clinical manifestations of PCV have been more deeply understood. Through dynamic observation and histopathological study of PCV lesions during operation, it can be confirmed that the lesions are mainly located between the retinal pigment epithelium and the Bruch membrane, rather than originating from the choroidal circulation, which is of great significance for understanding the origin and natural course of PCV. It is worth noting that although a theoretical bridge has been established between age-related macular degeneration (AMD)/PCV, there is a lack of intuitive clinical data on the ultrastructural and molecular manifestations of the cells/stroma in the local lesions of the eye, especially the progression of AMD/PCV from early/middle stage to exudative stage. It is precisely because of this that highly attractive research topics and exploration space are proposed for the future.