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find Keyword "细胞外信号调节MAP激酶类" 3 results
  • 丝裂原活化蛋白激酶信号通路与眼底新生血管性疾病相关性的研究进展

    丝裂原活化蛋白激酶(MAPK)信号通路中主要存在3种亚型,分别为细胞外信号调节激酶(ERK)、c-Jun氨基末端激酶(JNK)和p38 MAPK。它们在各亚群内部均存在着类似的、相互独立的三级级联反应,在适当刺激因素下作用于不同的底物可产生不同的细胞生物学效应。眼底新生血管是多种致盲眼病的病理基础,是多种因子相互作用导致促血管生成因子和抗血管生成因子间的平衡失调的结果;而有关多种因子发挥生物效应的MAPK信号通路在眼底新生血管发生发展过程中的作用越来越引起注意。MAPK信号通路在糖尿病视网膜病变、早产儿视网膜病变、老年性黄斑变性、视网膜静脉阻塞等疾病的新生血管形成中发挥重要的调控作用。通过对MAPK信号通路在眼底新生血管作用机制的探索,有助于深入详尽地了解眼部疾病的形成和发展规律,为预防和控制眼底新生血管形成和发展提供新的思路和方案。在未来,针对MAPK信号通路的靶向治疗将成为有效抑制眼底新生血管形成的重要治疗方案之一。

    Release date:2016-09-02 05:22 Export PDF Favorites Scan
  • The influence of Cisplan on the expression of B7-H1 in retinoblastoma cells

      Objective To observe the influence of cisplan on the expression of B7-H1 in retinoblastoma (RB) cells,and to investigate its mechanism. Methods Human RB cell line HXO-Rb44 cells were treated by 6 different concentrations of cisplan (0.000, 0.375, 0.750, 1.500, 3.000, 6.000 mu;g/ml), and their B7-H1 mRNA expression was determined by the reversetranscription polymerase chain reaction (RT-PCR) and fluorescence quantitative PCR (FQ-PCR); the B7-H1 protein expression was determined by immunofluorescence and flow cytometry. HXO-Rb44 cells were treated by 1.5 mu;g/ml cisplan for 0, 15, 30, 60, 120 min, then the phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2) was detected by Western blot.Results The expression of B7-H1 mRNA and protein in the 0.375, 0.750, 1.500, 3.000, 6.000 mu;g/ml group were significantly higher than that of the blank control group (F=395.478,112.03; P=0.000). Western blot showed that cisplan (1.5 mu;g/ml) could activate ERK1/2 by increasing its phosphorylation in HXO-Rb44 cells. After cisplan treatment, the phosphorylation of ERK1/2 increased gradually and reached its peak at 30 min, and then went down gradually.Conclusion Cisplan can promote the expression of B7-H1 and activate ERK1/2 in RB cells.

    Release date:2016-09-02 05:37 Export PDF Favorites Scan
  • Inhibitory effects of 5-lipoxygenase on oxygen-induced retinal neovascularization in mice

    ObjectiveTo investigate the inhibitory effects of 5-lipoxygenase (5-LOX) on oxygen-induced retinal neovascularization in mice and to explore its possible mechanisms. Methods7-day-old C57BL/6J mice were randomly divided into normal group, oxygen induced retinopathy (OIR) model group, large-dose group, small-dose group and control group with 12 mice in each group. The mice with their mothers were kept in (75±2)% of oxygen environment for 5 days and then returned to normoxia for 5 days to establish the OIR model except for normal group. From postnatal day 12 to 17, the large-dose group and small-dose group received intravitreous injection of 5-LOX at dose of 100 mg/kg and 50 mg/kg respectively, while the control group received the same volume of 1% dimethyl sulfoxide. The mice in the OIR group received no treatment. The number of endothelium cell nuclei breaking through the inner limiting membrane (ILM) was counted on hematoxylin and eosin-stained retinal section. The mRNA expression of 5-LOX, vascular endothelial growth factor (VEGF)-a, VEGF receptor 2 (VEGFR-2) on retinal tissue were detected by reverse transcription polymerase chain reaction (RT-PCR). The protein expression of 5-LOX, VEGF-a, VEGFR-2 and phosphorylation extracellular signal-regulated kinase (P-ERK) 1/2 on retinal tissue were detected by Western blot. ResultsThe number of vascular cell nuclei breaking through the ILM in the large-dose group and small-dose group decreased significantly compared with the OIR group and control group (F=73.390, P < 0.05). The mRNA expression and protein expression of 5-LOX, VEGFa, VEGFR-2 on retinal tissue were decreased significantly in the large-dose group and small-dose group as compared with the OIR group and control group (F=92.668, P < 0.05). The difference of VEGFR-2 protein expression between large-dose group and small-dose group was not significant (F=2.118, P > 0.05). The differences of 5-LOX, VEGF-a, P-ERK 1/2 protein expression between large-dose group and small-dose group were significant (F=86.490, 165.128, 139.424; P < 0.05). ConclusionHypoxia may induce 5-LOX expression in the retina. Retinal neovascularization was significantly inhibited by selective inhibition of 5-LOX.

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