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find Keyword "细胞间黏附分子-1" 15 results
  • The Effects of Modified Ultrafiltration on the Concen tration Changes of Soluble In tercellular Adheson Molecules and Tumor Necros is Factor α after Cardiopulmonary Bypass

    Abstract:  Objective  To investigate the effect of modified ultrafiltration on attenuating the inflammatory reaction and endothelial cell activation or damage after cardiopulmonary bypass (CPB).  Methods Forty patients undergoing cardiac operation with CPB were randomly divided into two groups. Ult rafiltration group ( n = 20) : patients underwent modified ultrafiltration after CPB; control group ( n = 20 ) : without ultrafiltration. Plasma concentrations of soluble intercellular adhesion molecules-1 ( s ICAM -1) and tumor necrosis factor-α (TNF-α) were determined with enzyme linked immunosorbent assay and radioimmunity pre-operat ively (baseline) , at the end of CPB, 4h and 24h post-operatively in both groups.  Results The concentrations of sICAM -1 in the cont rol group at 4h and 24h po st-operatively were higher than those pre-operatively (P lt; 0. 01). The concentrations sICAM -1 in the ultrafiltrat ion group in pre-operatively and at the end of CPB were not significantly different from that of the control group, but they were lower at 4h and 24h post-operatively (269. 6±33. 8Lg/L vs. 409. 6±37. 3Lg/L , 245. 9±32. 2Lg/L vs. 379. 3±35. 7Lg/L ; P lt; 0. 01). In the ultrafiltration group, the concentration of TN F-α at the end of CPB and 4h post-operatively were higher than that pre-operatively (P lt; 0. 01). The concent rations of TNF-α in the ultrafiltration group at 24h post-operatively recoved to the pre-operative level (0. 177±0. 024Lg/L vs. 0. 172±0. 030Lg/L ; P gt;0.05). In the control group, the concentration of TN F-α was higher at the end of CPB than that pre-operatively (P lt;0.01) , and decreased slightly at 4h and 24h post-operatively, but remained higher than those pre-operat ively (0. 264±0.045Lg/L vs. 0.174±0.033Lg/L , 0.218±0.028Lg/L vs. 0.174±0. 033Lg/L ; P lt; 0. 05).  Conclus ion CPB is known to induce inflammatory reaction and endothelial cell activation or damage. Modified ultrafiltration appears to attenuate these adverse reactions and is beneficial to postoperative convalescence.

    Release date:2016-08-30 06:08 Export PDF Favorites Scan
  • Preventive Effect of n-3 Polyunsaturated Fatty Acids on Allograft Arteriosclerosis

    Abstract: Objective To study the preventive effect of n-3 polyunsaturated fatty acids on allograft arteriosclerosis. Methods Arterial homeotransplant model were created with 480 rats which were divided into four groups. Control group, no n-3 lyunsaturated fatty acids were taken. Group A, n-3 polyunsaturated fatty acids were taken for two weeks before operation with the dose of EPA 600mg/kg. Group B, 300 mg/kg and group C 150 mg/kg were taken respectively. The recipient’s transplanted vessel was excised after 1,7,14,21and 28 days respectively. The tissue pathological variations, ultrastructure variations and expression variations of intercellular adhesion molecule-1(ICAM-1), vascular cell adhesion molecule-1(VCAM-1), nuclear factorkappa B(NF-κB) had been observed. Results The pathological changes occurred 7 days after operation in control group and were most prominent on the 28th day, blood vessels were obstructed and the expressions of ICAM-1, VCAM1,NF-κB were markedly intensified than those of group A, B, C (Plt;0.05). The pathological variations of transplanted vessel in group A, B, C occurred later than those in control group. The nonobstruction rates in group A, B, C were better than that in control group. The expressions of ICAM-1, VCAM-1, NF-κB in control group were ber than those in group A, B, C (Plt;0.05). The expressions of ICAM-1, VCAM-1, NF-κB after 1 day or 7 days demonstrated no statistically significant change in group A, B, C (Pgt;0.05). The preventive effect for allograft vessel atheromatosis in group A and group B was ber than that in group C after 14, 21 and 28d (Plt;0.05). There were no significant difference between group A and group B (Pgt; 0.05). Conclusion The n-3 polyunsaturated fatty acids can prevent the allograft vessel atheromatosis, the most effective dose of n-3 polyunsaturated fatty acids is 300 mg/kg.

    Release date:2016-08-30 06:15 Export PDF Favorites Scan
  • ACEI对心瓣膜置换术后患者s-ICAM-1、s—VCAM-1和v—WF含量的影响

    目的 观察血管紧张素转化酶抑制剂(ACEI)对风湿性心脏病心瓣膜置换术后患者血浆中可溶性黏附分子含量和心功能的影响。方法 85例风湿性心脏瓣膜病患者行心瓣膜置换术后被分为雅施达组(28例)、卡托普利组(29例)和对照组(28例),分别给予雅施达、卡托普利和安慰剂治疗。于手术后4周、8周抽血测定血浆中可溶性细胞间黏附分子-1(s—ICAM-1)、可溶性血管细胞问黏附分子-1(s—VCAM-1)和血管性血友病因子(v—WF)的含量,并同时测定心功能。结果 雅施达组和卡托普利组患者的s—ICAM-1、s—VCAM-1和v—WF浓度均低于对照组(P〈0.05),术后4周心功能恢复较对照组好(P〈0.05),但雅施达组和卡托普利组间比较差别无统计学意义(P〉0.05)。结论 ACEI能降低风湿性心脏病患者术后血浆中可溶性黏附分子水平,加强心瓣膜置换术后心脏功能恢复;而与ACEI的种类无明显的关系。

    Release date:2016-08-30 06:18 Export PDF Favorites Scan
  • 心脏不停跳手术中心肌NF-κB转录活性、ICAM-1的表达及其临床意义

    目的 探讨心脏不停跳与心脏停搏手术对心肌核转录因子κB(NF-κB)转录活性、细胞间黏附分子-1(ICAM-1)表达水平的影响及其临床意义。方法 将40例先天性心脏病患者随机分为两组,每组20例。组Ⅰ:行心脏不停跳心内直视手术;组Ⅱ:行常规体外循环手术(灌注冷晶体心脏停搏液)。两组患者均于心内操作前、后取右心房壁心肌组织检测NF-κB转录活性、ICAM-1表达水平,用透射电子显微镜观察心肌超微结构;分别于术前、主动脉开放或心内操作完成后1、24、48和72h测定两组心肌肌钙蛋白I(cTnI)、肌酸激酶同工酶MB(CK-MB),并对其结果进行比较。结果术后组Ⅰ NF-κB转录活性、ICAM-1表达水平较术前无显著变化,组Ⅱ NF-κB转录活性较术前升高(Plt;0.01);术后NF-κB转录活性组Ⅱ显著高于组Ⅰ(Plt;0.01)。术后两组血清cTnI、CK-MB水平较术前均有不同程度升高(Plt;0.01),主动脉开放后/心内操作完成后各时点,组Ⅱ均显著高于组Ⅰ(Plt;0.01)。透射电子显微镜观察,组Ⅰ术后心肌超微结构无明显变化,组Ⅱ心肌损伤变化显著。结论 心脏不停跳下心内直视手术术后短期内心肌NF-κB转录活性、ICAM-1表达水平无明显变化,减轻了心肌缺血-再灌注损伤及由NF-κB激活而引起的心肌炎性反应,有较好的心肌保护效果。

    Release date:2016-08-30 06:23 Export PDF Favorites Scan
  • The effect of PDTC on ICAM-1,VCAM-1 expression of endothelial cells in hypoxia/reoxygenation-stimulated

    Objective To study the mechanisms and treatment of ischemia /reperfusion injury, expression of intracellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1) were measured, the effect on suppression of ICAM-1 and VCAM-1 by the pyrrolidine dithiocarbamate (PDTC) were investigated. Methods Endothelial cells were divided into 3 groups, hypoxia group: endothelial cells were exposed in hypoxia condition, then returned to reoxygenation condition; the PDTC group: PDTC was added to the endothelial cells in the culture media before exposing to hypoxia condition; control group: endothelial cells underwent treatment. Confocal microscopy was used to detect expression of ICAM-1 and VCAM-1. Results ICAM-1 and VCAM-1 expression were low in endothelial cells of control group, and increased in hypoxia group . ICAM-1 and VCAM-1 expression of endothelial cells in PDTC group werelower than those in hypoxia group , but higher than those in control group. Conclusions It seems that hypoxia/ reoxygenation can activate the endothelial cells and increase the expression of cell adhesion molecules. PDTC can decrease the expression of ICAM-1 and VCAM-1. PDTC may prove benificial in the treatment of ischemia /reperfusion injury.

    Release date:2016-08-30 06:27 Export PDF Favorites Scan
  • The protective effect of N-acetyl-L-cysteine on donor rat lung in the period of reperfusion

    Objective To observe the protective effect on rat lung by using N-acetyl-L-cysteine(NAC) a inhibiter of nuclear factor-kappa B (NF-κB) in the period of reperfusion. Methods Twenty-four rats were randomly divided into a control group and a trail group.The harvested lung blocks of 12 rats were flushed with and stored in the low-potassium-dextran (LPD) solution at 4℃ for 16 hours. The isolated rat lung reperfusion models were established and the donor lungs were perfused for 1 hour. NAC was used in the trail group but normal saline was used in control group. Partical pressure of oxygen in artery (PaO2), peak airway pressure (PawP) were measured at every 15 min intervals during reperfusion. After reperfusion, the lung tissue wet-to-dry(W/D)ratio, and myeloperoxidase(MPO) activity were obtained. The protein and mRNA expressions of intercellular adhesion molecule-1(ICAM-1), NF-κB were also observed by using immunohistochemistry and semi-quantitative RT-PCR at the end of reperfusion. Results The level of decreased PaO2 and increased PawP in trail group were lower than those in control group at every interval time the sample obtained after reperfusion in 60 min. (Plt;0.01 or lt;0.05). After reperfusion the W/D,MPO, the protein and mRNA expressions of ICAM-1, NF-κB were decreased evidently in trail group than those in control group(Plt;0.01 or lt;0.05). Conclusion Using NAC in the period of reperfusion, can effectively inhibit the expression of NF-κB and ICAM-1,further improve lung respiratory functions.

    Release date:2016-08-30 06:28 Export PDF Favorites Scan
  • Effects of Isoflurane on the Changes of Oxygen Free Radicals and Intercellular Adhesion Molecule-1 Expression in Rats with Liver Ischemia and Reperfusion Injury

    目的 探讨异氟醚通过抑制细胞间黏附分子(ICAM-1)表达参与减轻肝脏缺血-再灌注(IR)损伤的可能调节机制。 方法 32只雌性SD大鼠分为4组。A组大鼠行腹腔注射1%戊巴比妥钠40 mg/kg麻醉,进行手术但不阻断入肝血流;B组1%戊巴比妥钠麻醉后行部分肝脏IR;C组大鼠仅接受1.0 MAC异氟醚吸入麻醉,不阻断血流;D组采用1.0 MAC异氟醚麻醉,建立肝脏IR模型。肝脏缺血60 min,再灌注3 h后取肝组织和血液标本,检测血清丙氨酸转氨酶(ALT)和天冬门氨酸转氨酶(AST)、肝组织ICAM-1和肝组织还原型谷胱甘肽(GSH)、脂质过氧化物丙二醛(MDA)和超氧化物歧化酶(SOD)含量。 结果 与戊巴比妥钠麻醉比较,采用异氟醚处理后明显降低血清ALT和AST的水平,再灌注肝组织内GSH、SOD含量明显高于而MDA含量降低,同时抑制肝组织ICAM-1的表达。 结论 异氟醚麻醉能够有效减轻肝脏IR损伤,抑制氧自由基的生成和释放,具体机制可能与抑制ICAM-1表达致使细胞内GSH含量增加密切相关。

    Release date:2016-09-08 09:11 Export PDF Favorites Scan
  • Expression of ICAM-1 mRNA of Isolated Rat Liver after Preservation and Reperfusion and Effect of Salvia Miltiorrhiza on Its Expression

    目的 探讨大鼠离体肝脏保存再灌注后肝脏组织中细胞间黏附分子-1(ICAM-1) mRNA的表达变化及丹参对其表达的影响。 方法 选取健康Wistar雄性大鼠54只,用完全随机方法选6只大鼠作为正常组,切除肝脏后立即灌注;随机选24只大鼠作为对照组,切除肝脏后置入4 ℃UW液中分别保存8、16、24、32h后再行肝脏循环再灌注;余下的24只作为实验组,切除肝脏后置入含丹参的4 ℃UW液中分别保存8、16、24、32h后再行肝脏循环再灌注。应用 RT-PCR方法检测各组大鼠离体肝脏保存再灌注后肝脏组织中ICAM-1mRNA表达。 结果 正常组肝脏中的ICAM-1mRNA表达为3.61±1.56,对照组和实验组8、16、24、32h时肝脏中ICAM-1mRNA表达分别为15.71±1.78、33.70±3.35、45.83±4.37、66.98±5.89和11.69±1.25、16.55±1.37、24.73±2.74、32.65±3.39,对照组和实验组各时相均分别明显低于正常组(P<0.05),且均随保存时间延长,ICAM-1mRNA 表达逐渐增加(P<0.05),实验组16h后ICAM-1mRNA 表达均分别明显低于对照组相应时相(P<0.05)。结论 丹参能够降低离体肝脏保存再灌注后肝脏组织中ICAM-1mRNA表达,对肝脏保存再灌注损伤可能具有防护作用。

    Release date:2016-09-08 10:35 Export PDF Favorites Scan
  • Effect and Mechanism of Glutamine to The Aberrant Crypt Foci in Rats

    Objective To explore the effect and mechanism of glutamine to the aberrant crypt foci (ACF) in rat injured by acetic acid. Methods Thirty Wistar rats were averagely divided into three groups: control group, acetic acid group and glutamine group. The colon of the rat was infused with 1% acetic acid. Started to gavage with glutamate two days after modeling glutamine group. The injured colons were studied after fourteen days with light and scanning electronic microscope. Paraffin sections of specimens were prepared and stained with HE. The colon crypts were isolated by HCl digestion method. The expressions of CD44 and ICAM-1 in the epithelial cell of the large intestine mucosa were detected by immunohistochemistry method. Results On the days of 14, the number of ACF in the glutamine group were remarkably decreased as compared with that of the acetic acid group and a branch-like. The expressions of ICAM-1 and CD44 (every 1 000 cells) were 302.1±30.1 and 298.6±28.3 in glutamine group, 223.6±23.5 and 221.5±28.6 in control group, 198.5±19.5 and 215.3±17.8 in acetic acid group, respectively. While the expressions of CD44 and ICAM-1 in intestine were increased remarkably in the glutamine group compared with the control group and acetic acid group (P<0.05). Conclusion  Glutamine could decrease the formation of the ACF injured by acetic acid. Increasing the expressions of CD44 and ICAM-1 may be one of the important factors to decrease the ACF.

    Release date:2016-09-08 11:47 Export PDF Favorites Scan
  • Effects of Newcastle Disease Virus and Adriamycin on Surface Structure and Actin of Hepatocellular Carcinoma Cell Lines SMMC-7721

    【Abstract】ObjectiveTo compare the effects of newcastle disease virus (NDV) and adriamycin (ADM) on surface structure and actin of hepatocellular carcinoma cell lines SMMC-7721. Methods SMMC-7721 carcinoma cell lines were divided into 2 groups. NDV was added into one group, while ADM was added into the other group. The cells were then cultured at 5 time phases (8, 16, 24, 36 and 48 h). Intracellular actin and Ca2+ were examined by using immunofluorescence method. CD44 and intercellular adhesion molecule-1 (ICAM-1) were detected by using immunochemical method and flow cytometry, respectively. The change of cellular surface structure was observed by scan electron microscope. Results Cells gradually contracted and turned round over time. It was observed that actin was segmented and cells alignment became disordered. The mean fluorescence intensity of actin decreased in both groups, but it was obvious in NDV group. There were significant differences of fluorescence intensity between 2 groups at the phases of 16 h (P<0.05), 24 h (P<0.05), 36 h (P<0.01) and 48 h (P<0.05), except the one after 8 h. Intracellular Ca2+ concentration increased gradually in both groups, and the amplifications in NDV group were significantly higher at the phases of 24 h, 36 h and 48 h than those in ADM group (P<0.01, P<0.05 and P<0.01, respectively). There were also differences at 8 and 16 h, but there were no statistical significance. The expression of CD44 in cells decreased. The mean fluorescence intensity of ICAM-1 raised gradually, and then came to peaking at 36 h, but there was no significant difference between two groups. All the above indices between different phases in the same group showed significant differences (P<0.05). Conclusion Both NDV and ADM could make tumor cells degenerate and rupture, but the effect of NDV is more intensive. It could increase the fragility of cells and hasten the process of cell rupture. Disintegrated cancer cell and changes of adhesion molecule could lead cancer cells be identified, encapsulated, and killed by immune cells under static condition.

    Release date:2016-09-08 11:45 Export PDF Favorites Scan
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