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find Keyword "缺血再灌注损伤" 5 results
  • 参附注射液对兔移植肺缺血再灌注损伤的保护作用

    目的 观察参附注射液对供肺缺血再灌注损伤的保护作用。 方法 将20只新西兰白兔随机分为实验组和对照组,每组各10只,建立兔左肺自体原位移植模型,分别用参附注射液和生理盐水对兔肺进行预处理和供肺灌注。于主动脉阻断前、再灌注后15 min、30 min和60 min各时点检测左肺静脉血中丙二醛(MDA)含量、总超氧化物歧化酶(SOD)的活力,于再灌注60 min后称左肺组织的干湿比重(D/W),并观察其病理变化。 结果 主动脉阻断前两组MDA含量差异无统计学意义(Pgt;0.05);再灌注15 min后实验组MDA含量较主动脉阻断前下降;再灌注30 min和60 min时,两组MDA含量均呈上升趋势,但实验组明显低于对照组(Plt;0.05)。主动脉阻断前实验组SOD活力明显高于对照组(Plt;0.05),再灌注后两组SOD活力均呈下降趋势,以对照组下降幅度明显(Plt;0.05)。实验组的D/W显著高于对照组(0.23±0.01 vs. 0.19±0.02,Plt;0.05)。对照组肺组织水肿明显,大量的炎性细胞浸润,肺泡腔内有片状渗出;而实验组表现为肺泡间隔水肿轻微,少量炎细胞浸润,渗出不明显。 结论 参附注射液对供肺的缺血再灌注损伤有较好的保护作用。

    Release date:2016-08-30 06:02 Export PDF Favorites Scan
  • The Protective Effects of Ischemic Postconditioning on Ischemiareperfusion Myocardium and the Relationship with Mitochondrial Adenosine Triphosphate Sensitive K+ Channels

    Objective To investigate the protective effects of ischemic postconditioning (IPo) on ischemiareperfusion (I/R) myocardium and the relationship with mitochondrial adenosine triphosphate (ATP) sensitive K+ channels (mitoKATP) and provide evidences to the development of druginduced postconditioning. Methods Langendorff models were established in 40 Wistar rats which were divided into 5 groups by random number table with 8 rats in each group. Normal control group(NC group): the rat hearts were continuously reperfused by KrebsHenseleit bicarbonate buffer (K-HB) for 100 min without any other treatment; I/R group: the rat hearts underwent a 40-min global ischemia followed by a 60-min reperfusion; IPo group: after a 40-min global ischemia, the process of 10-second reperfusion followed by a 10-second ischemia was repeated 6 times, then there was a continuous 58min reperfusion; 5-hydroxydecanoic acid(5-HD) group: after a 40min global ischemia, hearts with 5HD(100 μmol/L) K-HB were reperfused for 15min and then perfused without 5HD for 45min;IPo+5-HD group: after a 40-min global ischemia, the process that the isolated hearts with 5-HD(100 μmol/L) KHB were reperfused for 10second followed by a 10second ischemia was repeated 6 times, then the hearts with 5-HD(100 μmol/L) KHB were continuously [CM(159mm]perfused for 13-min followed by reperfusion without 5-HD(100 μmol/L) K-HB for 45-min. The cardiac function,coronary flow(CF), cardiac troponin I(cTnI) content in coronary effluent, the area of acute myocardial infarction (AMI) and myocardial ultrastructure were observed. Results Left ventricular developed pressure(74.3±3.3 mm Hg vs. 57.1±3.3 mm Hg,t=1300, P=0.000),+dp/dtmax(1 706.6±135.6 mm Hg/s vs. 1 313.3±96.2 mm Hg/s,t=6.28,P=0.000),-dp/dtmax(1 132.8±112.1 mm Hg/s vs. 575.7±67.7 mm Hg/s,t=13.48, P=0.000) and CF(6.49±0.30 ml/min vs. 3.70±0.24 ml/min,t=28.6,P=0.000) in IPo group were higher than those in I/R group. Left ventricular enddiastolic pressure(10.9±1.7mm Hg vs. 26.2±1.5 mm Hg,t=-19.21, P=0000)and cTnI content in coronary effluent (0.62±0.01 ng/ml vs. 0.71±0.01 ng/ml, t=-12.00,P=0.000) were lower than those in I/R group; the area of AMI decreased 20.8% compared with that in I/R group (Plt;0.05). The myocardial protective effect in IPo+5HD group was similar with that in IPo group, but lower than that in IPo group. The electron microscope showed that IPo and IPo+5HD could reduce myocardial fiber damage and mitochondrial damage caused by I/R. Conclusion IPo can protect I/R myocardium, which is achieved mainly by activating mitoK-ATP channels. 

    Release date:2016-08-30 06:06 Export PDF Favorites Scan
  • The Effect of Ischemic Preconditioning on Myocardial Ischemic Reperfusion of Elderly Rats

    Objective To investigate the effect of ischemic preconditioning(IPC) on myocardial ischemic reperfusion injury(I/R) of elderly rats. Methods Fiftysix Wistar rats, of which there were 28 aged from 21-23months(elderly rat) and 28 aged from 4-5months(young rat), were used to build isolated heart perfusion Langendorff model. The rats were divided into 7 groups with random number table(8 in each group): adult control group, adult I/R group, adult IPC group, elderly control group, elderly I/R group, elderly IPC group and elderly enhanced IPC group. The control group underwent a 90-min perfusion without any intervention; the I/R group underwent a 30-min equilibration period, then a 30-min ischemia and a 30-min reperfusion; the IPC group underwent a 10-min equilibration period, then a 5-min ischemia for twice and a 5-min reperfusion, after that a 30-min ischemia and [CM(158-3mm]a 30-min reperfusion; the enhanced IPC group underwent a 10-min equilibration period, then a 5-min ischemia for 4-times and a 5-min reperfusion, after that a 30-min ischemia and a 30-min reperfusion. The recovery rates of cardiac output(CO), left ventricular developed pressure (LVDP), the maximum rising and descending rate of left ventricular pressure (±dp/dtmax) after a 30-min reperfusion were compared among groups. The activity of creatine kinase (CK) in coronary outflow, the level of malonyldialdehyde (MDA) and superoxide dismutase (SOD) before ischemia and after a 30min reperfusion were detected. The myocardial infarction areas were compared among groups. Results After a 30min reperfusion, compared with adult I/R group, in adult IPC group CK reduced significantly(89.48±18.72 U/L vs. 115.76±16.72 U/L,q=6.061,Plt;0.01),the level of MDA decreased significantly(9.53±3.44 nmol/ml vs. 16.84±2.29 nmol/ml,q=7.732,Plt;0.01),the level of SOD increased significantly(584.7±122.62 U/ml vs. 429.46±85.24 U/ml,q=4.754,Plt;0.01),the recovery rates of CO,LVDP,+dp/dtmax and -dp/dtmax increased ignificantly(78.69%±9.68% vs. 65.10%±8.63%,83.61%±8.46% vs. 67.23±8.68%,81.68±8.68% vs. 67.89%±6.89%,89.79%±7.78% vs. 66.79%±8.46%,Plt;0.01), the myocardial infarction areas reduced significantly (5.25%±4.33% vs. 14.75%±8.02%,q=7.458,Plt;0.01)。There was no statistical significance between elderly IPC group and elderly I/R group in the above indexes(Pgt;0.05).However, There was statistical significances between elderly enhanced IPC group and I/R group. CK reduced significantly (88.60±28.32 U/L vs. 105.76±9.64 U/L,q=5.620,Plt;0.01),the level of MDA decreased significantly(8.38±3.36 nmol/ml vs. 16.80±3.06 nmol/ml,q=7.500,Plt;0.01),the level of SOD increased significantly(558.87±78.66 U/ml vs. 433.75±86.65 U/ml,q=7.335,Plt;0.01),the recovery rates of CO,LVDP,+dp/dtmax and -dp/dtmax increased significantly (77.99%±10.02% vs. 66.26%±9.78%,85.59%±6.67% vs. 73.90%±6.66%,83.87%±9.98% vs. 68.90%±8.68%,86.01%±766% vs. 70.39%±7.98%,Plt;0.01), the myocardial infarction areas reduced significantly (795%±6.32% vs. 1568%±10.36%,q=8.680, Plt;0.01). 〖WTHZ〗Conclusion The protective effect of IPC on I/R elderly rat hearts has weakened. The enhanced IPC is able to regain the protective effect of IPC on elderly rat hearts.

    Release date:2016-08-30 06:06 Export PDF Favorites Scan
  • Protective Effects of Adenosine on Ischemia Reperfusion Injury of the Donor Lung Following Heart lung Transplantation in Canine

    Abstract: Objective To investigate the protective effects of adenosine (ADO) on lung ischemia/reperfusion injury following heart-lung transplantation in canine. Methods Canine heart-lung transplantation was performed.Canines were divided into two groups: transplant control groupand ADO group. The changes of arterial partial pressure of oxygen(PaO2) after reperfusion in two groups at 30,60,90,120 min were observed.The tissue contents of nitric oxide (NO) were measured at 10 min before ischemia, 10 min and 120 min after ischemia; 10 min and 60 min after reperfusion.The lung tissue samples were obtained 1h after reperfusion.The tissue myeloperoxidase(MPO) activity,content of malondialdehyde(MDA), content of superoxide dismutase(SOD), wet/dry ratio of lung(W/D) were measured.Microscopic examination of lungs was also conducted. Results (1)In ADO group,PaO2 were significantly higher than that in control group at 30,60,90 and 120 min after reperfusion (Plt;0.05).(2) The tissue contents of NO at 120 min after ischemia, 10 min and 60 min after reperfusion were significantly lower than that at 10 min before ischemia(Plt;0.05). In ADO group,the tissue contents of NO at 120 min after ischemia, 10 min and 60 min after reperfusion were higher than that in control group respectively(Plt;0.05). (3)The tissue MPO activity, content of MDA, W/D in ADO group were significantly lower than those in corresponding control group. The content of SOD in ADO group were higher than that in control group(Plt;0. 05).(4)The microscopic examination showed that there were severe leukocyte infiltration and edema formation in the alveolar space in control group, but the changes were less severe in ADO group. Conclusion Administration of ADO in canine heart-lung transplantation can protect the donor lung against ischemia/reperfusion injury.

    Release date:2016-08-30 06:13 Export PDF Favorites Scan
  • Changes of Somatosensory Evoked Potentials During Graded Spinal Cord Ischemia/reperfusion Injury in Rabbits

    Objective To investigate the relationship between graded spinal cord ischemia/reperfusion injury and somatosensory evoked potentials(SEP),neurologic function score(NFS)and the histopathological changes of spinal cord. Methods Forty rabbits were randomized and equally divided into 4 groups: shamoperation group, ischemia for 30min, 45min and 60min groups. The spinal cord ischemiareperfusion injury model was created by occlusion of the abdominal aorta in rabbits. SEP was monitored before ischemia,5,10minutes after ischemia, 15, 30 minutes, 1,2, 24 and 48 hours after reperfusion. NFS was evaluated at 6,12,24 and 48 hours after reperfusion.The pathological changes of spinal cord were observed after reperfusion 48 hours. Results The pathological characters with mild,moderate and severe spinal cord ischemia/reperfusion injury could be simulated by declamping after 30, 45 and 60 minutes infrarenal aorta crossclamping. SEP amplitude returned to normal after reperfusion 15 minutes(Pgt;0.05)and SEP latency returned to normal after reperfusion 30 minutes(Pgt;0.05)during mild spinal cord ischemia/reperfusion injury.SEP amplitude returned to normal after reperfusion 30 minutes(Pgt;0.05)and SEP latency returned to normal after reperfusion 60 minutes(Pgt;0.05)during moderate spinal cord ischemia/reperfusion injury. SEP latency increased and SEP amplitude decreased during severe spinal cord ischemia/reperfusion injury,compared with other groups, there were significant differences in SEP latency and SEP amplitude by clamping the infrarenal aorta for 60min(Plt;0.01). With graded spinal cord ischemia/reperfusion injury, compared with shamoperation group, spinal cord ischemiareperfusion groups had significant differences in NFS(Plt;0.01). Conclusion SEP is much quicker in the recovery of amplitude than latency during spinal cord ischemia/reperfusion. SEP is a sensitive and accurate index for spinal cord function during ischemia/reperfusion injury. SEP monitoring spinal cord ischemia/reperfusion injury during operation provides experimental basis for clinical application.

    Release date:2016-08-30 06:16 Export PDF Favorites Scan
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