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find Author "聂开瑜" 29 results
  • 拇指背侧皮神经营养血管筋膜蒂逆行皮瓣修复拇指指腹缺损

    目的 总结采用拇指背侧皮神经营养血管筋膜蒂逆行皮瓣移位修复拇指指腹缺损的临床效果。 方 法 2006 年1 月- 2008 年3 月,收治拇指指腹缺损24 例。男18 例,女6 例;年龄16 ~ 56 岁,平均26.5 岁。爆炸伤4 例,机器绞伤8 例,电锯伤12 例。缺损范围为2.0 cm × 2.0 cm ~ 3.5 cm × 3.0 cm。受伤至入院时间30 min ~ 7 h。术中切取2.5 cm × 2.5 cm ~ 4.0 cm × 3.5 cm 拇指背侧皮神经营养血管筋膜蒂逆行皮瓣修复缺损。供区宽度lt; 2.8 cm 者直接缝合,gt; 2.8 cm 者于前臂内侧切取皮片游离移植修复。 结果 术后21 例皮瓣完全成活;3 例术后48 h 因静脉回流障碍远端部分坏死,经换药后成活。供区切口顺利愈合,植皮均成活。18 例获随访,随访时间6 ~ 12 个月。皮瓣质地优良不臃肿,外形满意;两点辨别觉为5 ~ 12 mm。 结论 吻合神经的拇指背侧皮神经营养血管筋膜蒂逆行皮瓣外形好,术后感觉恢复满意,是修复拇指指腹缺损的一种理想方法。

    Release date:2016-09-01 09:06 Export PDF Favorites Scan
  • 第五掌骨桡背侧逆行筋膜蒂岛状皮瓣修复环小指皮肤缺损

    目的 总结第5 掌骨桡背侧逆行筋膜蒂岛状皮瓣修复环、小指皮肤缺损的手术方法及疗效。 方法 2004 年9 月- 2009 年10 月,应用第5 掌骨桡背侧逆行筋膜蒂岛状皮瓣修复环、小指皮肤缺损26 例。男18 例,女8 例;年龄18 ~ 56 岁,平均28.5 岁。机器绞伤12 例,电刨伤6 例,电锯伤8 例。损伤指别:环指10 例,小指16 例。损伤部位:掌侧皮肤缺损8 例,指背皮肤缺损18 例。创面范围2.5 cm × 1.5 cm~ 4.5 cm × 3.0 cm。受伤至手术时间1 ~ 9 h,平均4.5 h。术中皮瓣切取范围为3.0 cm × 2.0 cm ~ 5.0 cm × 3.5 cm。供区直接缝合或植皮修复。 结果 术后3 ~ 5 d 4 例皮瓣远端发生静脉回流障碍,发生张力性水疱,经换药处理后表皮脱落愈合;其余皮瓣及供区植皮均顺利成活,切口均Ⅰ期愈合。26 例术后均获随访,随访时间6 ~ 24 个月,平均12 个月。其中10 例因皮瓣臃肿于术后6 ~ 8 个月行皮瓣整形术。末次随访时皮瓣外形均满意,质地与受区相似,两点辨别觉为0.8 ~ 1.2 cm。掌指关节屈曲达80 ~ 90°,平均84°;近侧指间关节屈曲达80 ~ 100°,平均90°。 结论 第5 掌骨桡背侧逆行筋膜岛状皮瓣具有血供可靠、切取方便、质地良好等优点,是修复环、小指皮肤缺损的一种较好方法。

    Release date:2016-08-31 05:41 Export PDF Favorites Scan
  • (足母)趾腓侧皮瓣游离移植修复手指掌侧皮肤缺损

    目的 总结(足母)趾腓侧皮瓣游离移植修复手指掌侧皮肤缺损的疗效。 方法 2005 年7 月-2009 年9 月,收治手指掌侧皮肤缺损20 例。男16 例,女4 例;年龄18 ~ 56 岁,平均28.5 岁。致伤原因:机器绞伤12 例,爆炸伤5 例,感染引起局部坏死3 例。拇指14 例,示指3 例,中指3 例。17 例外伤创面有不同程度污染,伤后至入院时间为1 ~ 6 h;3 例感染创面发生局部皮肤坏死,伤后至该次入院时间为15 ~ 53 d。创面缺损范围3.0 cm × 2.0 cm ~ 3.5 cm × 2.5 cm。术中采用大小为3.5 cm × 2.5 cm ~ 4.0 cm × 3.0 cm 的(足母)趾腓侧皮瓣游离移植修复创面,供区植皮修复。 结果 1 例术后2 d 因动脉危象导致皮瓣坏死,行邻指指固有动脉侧方皮瓣移位修复后成活;其余皮瓣及植皮均顺利成活,创面均Ⅰ期愈合。术后19 例获随访,随访时间6 ~ 18 个月,平均12 个月。手指外形、质地较好。末次随访时示、中指掌指关节屈曲达80 ~ 90°,近侧指间关节屈曲达80 ~ 100°,远侧指间关节屈曲达20 ~ 30°;拇指指间关节屈曲30 ~ 80°;对指、对掌功能不受限。供足行走正常。 结论 (足母)趾腓侧皮瓣游离移植修复手指掌侧皮肤缺损具有质地结构相似、精细感觉恢复优良、不需二次整形等优点,是有效治疗方法之一。

    Release date:2016-08-31 05:43 Export PDF Favorites Scan
  • 足背岛状动脉皮瓣修复小腿中下段软组织缺损

    Release date:2016-09-01 09:26 Export PDF Favorites Scan
  • Effects of rapamycin and deferoxamin on wound healing after ischemia and hypoxia

    Objective To explore the effect and mechanism of rapamycin and deferoxamin on wound healing after ischemia and hypoxia. Methods The model of ischemia and hypoxia wound was made on the back of 40 SPF male adult Sprague Dawley rats, weight (300±20) g; they were randomly divided into 4 groups (n=10): the control group (group A), deferoxamine intervention group (group B), rapamycin intervention group (group C), and deferoxamine+rapamycin intervention group (group D). At 3, 6, and 9 days after model preparation, rats of groups A, B, C, and D were intra-peritoneally injected with normal saline, deferoxamin (10 mg/kg), rapamycin (3 mg/kg), deferoxamin (10 mg/kg)+rapamycin (3 mg/kg) respectively. The wound healing was observed and the healing time was recorded in each group; the wound healing tissue was harvested to test the mRNA and protein expressions of mammalian target of rapamycin (mTOR), hypoxia inducible factor 1α (HIF-1α), and vascular endothelial growth factor (VEGF) by real-time fluorescence quantitative PCR and Western blot at 2 days after wound healing. Results All rats survived to the end of the experiment, and wounds healed; the healing time of groups A, B, and D was significantly shorter than that of group C (P<0.05), but there was no significant difference between groups A, B, and D (P>0.05). Real-time fluorescence quantitative PCR showed that the expression of mTOR mRNA in groups C and D was significantly decreased when compared with the expressions in groups A and B (P<0.05); there was significant difference between groups A and B (P<0.05), but no significant difference between groups C and D (P>0.05). The expressions of HIF-1α mRNA and VEGF mRNA were signi-ficantly higher in groups B and D than groups A and C, and in group A than group C (P<0.05), but there was no signifi-cant difference between groups B and D (P>0.05). Western blot showed that the relative expressions of mTOR protein in groups C and D were significantly decreased when compared with the expressions in groups A and B (P<0.05), but there was no significant difference between groups C and D (P>0.05). The relative expressions of HIF-1α protein in groups A, B, and C were significantly increased when compared with expression in group D (P<0.05), but there was no significant difference between groups A, B, and C (P>0.05). The relative expression of VEGF protein were significantly lower in groups B, C, and D than group A, in group D than groups B and C, and in group C than group B (P<0.05). Conclusion Defe-roxamin can promote the wound healing of rats after ischemia and hypoxia, and the effect of rapamycin is opposite. It may be related to the existence of mTOR and HIF-1 signaling pathway in chronic ischemia-hypoxia wound.

    Release date:2017-06-15 10:04 Export PDF Favorites Scan
  • The role of Schwann cells-like cells derived from human amniotic membrane mesenchymal stem cells transplantation in flap nerves regeneration

    Objective Inducing human amniotic membrane mesenchymal stem cells (hAMSCs) to Schwann cells-like cells (SCs-like cells) in vitro, and to evaluate the efficacy of transplantation of hAMSCs and SCs-like cells on nerves regeneration of the rat flaps. Methods hAMSCs were isolated from placenta via two-step digestion and cultured by using trypsin and collagenase, then identified them by flow cytometry assay and immunofluorescence staining. The 3rd generation of hAMSCs cultured for 6 days were induced to SCs-like cells in vitro; at 19 days after induction, the levels of S-100, p75, and glial fibrillary acidic protein (GFAP) were detected by immunofluorescence staining, Western blot, and real-time fluorescence quantitative PCR (qPCR). The levels of brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) were measured by ELISA in the supernatant of the 3rd generation of hAMSCs cultured for 6 days and the hAMSCs induced within 19 days. In addition, 75 female Sprague Dawley rats were taken to establish the rat denervated perforator flap model of the abdominal wall, and were divided into 3 groups (n=25). The 3rd generation of hAMSCs (1×106 cells) in the proliferation period of culturing for 6 days, the SCs-like cells (1×106 cells), and equal volume PBS were injected subcutaneously in the skin flap of the rat in groups A, B, and C, respectively. At 2, 5, 7, 9, and 14 days after transplantation, 5 rats in each group were killed to harvest the flap frozen sections and observe the positive expression of neurofilament heavy polypeptide antibody (NF-01) by immunofluorescence staining. Results The cells were identified as hAMSCs by flow cytometry assay and immunofluorescence staining. The results of immunofluorescence staining, Western blot, qPCR showed that the percentage of positive cells, protein expression, and gene relative expression of S-100, p75, and GFAP in SCs-like cells group were significantly higher than those in hAMSCs group (P<0.05). The results of ELISA demonstrated that the expression of BDNF and NGF was significantly decreased after added induced liquid 1, and the level of BDNF and NGF increased gradually with the induction of liquids 2 and 3, and the concentration of BDNF and NGF was significantly higher than that of hAMSCs group (P<0.05). Immunofluorescence staining showed that the number of regenerated nerve fibers in group B was higher than that in groups A and C after 5-14 days of transplantation. Conclusion The hAMSCs can be induced into SCs-like cells with the proper chemical factor regulation in vitro, and a large number of promoting nerve growth factor were released during the process of differentiation, and nerve regeneration in flaps being transplanted the SCs-like cells was better than that in flaps being transplanted the hAMSCs, which through a large number of BDNF and NGF were released.

    Release date:2018-01-09 11:23 Export PDF Favorites Scan
  • Superficial temporal artery composite perforator flap in repair of temporal skin and soft tissue defects and reconstruction of sideburns

    ObjectiveTo investigate the feasibility and effectiveness of repairing temporal skin and soft tissue defects and reconstructing sideburns with superficial temporal artery composite perforator flap.MethodsBetween January 2018 and January 2019, 12 patients with temporal tumors were treated. There were 5 males and 7 females with an average age of 51 years (range, 37-68 years). There were 8 cases of basal cell carcinoma and 4 cases of squamous cell carcinoma. The disease duration ranged from 3 months to 4 years (mean, 13 months). The area of residual wound after tumor resection was 3.8 cm×2.5 cm-5.2 cm×3.5 cm. The superficial temporal artery composite perforator flap was designed. The hairy superficial temporal artery frontal branch perforator flap was used to repair the hair growing area and reconstruct the sideburn; and the area of the flap was 2.5 cm×1.0 cm-4.2 cm×3.0 cm. And the superficial temporal artery descending branch perforator flap without hair was used to repair the hair-free area; and the area of the flap was 2.5 cm×1.5 cm-7.5 cm×4.0 cm. The donor sites were sutured directly.ResultsAll flaps survived, and the incisions at the donor and recipient sites healed by first intention. Eleven patients were followed up 6-12 months (mean, 9 months). The incisions were not obvious. The flaps were flat and the color of the flaps were not significantly different from the surrounding skin. The reconstructed sideburns were consistent with the healthy side and the facial appearance was satisfactory. No local tumor recurred during follow-up.ConclusionFor the temporal skin and soft tissue defects involving the sideburn, the superficial temporal artery composite perforator flap can be used to repair subunits with different aesthetic characteristics in sections and has the advantages of operating simply, obtaining satisfied facial appearance, and little effect on the donor site.

    Release date:2020-04-29 03:03 Export PDF Favorites Scan
  • Free vastus lateralis flap combined with skin grafting for repairing small- and medium-sized lacunar defects in non-weight-bearing area of diabetic foot

    Objective To explore the feasibility and effectiveness of free vastus lateralis flap combined with skin grafting for repairing small- and medium-sized lacunar defects in the non-weight-bearing area of diabetic foot. Methods Between January 2022 and October 2023, 8 patients (8 feet) with small- and medium-sized lacunar defects in the non-weight-bearing area of diabetic foot were admitted. There were 6 males and 2 females, with an average age of 64.3 years (range, 58-76 years). The duration of the diabetic foot ulcer ranged from 2 to 7 weeks (mean, 4.3 weeks). The wound was located between the metatarsal bones in 4 cases, on the medial side of the foot in 2 cases, on the lateral side of the foot in 1 case, and on the dorsal and lateral sides of the foot in 1 case. The length of wound was 4.0-12.0 cm, the width was 3.0-5.0 cm, and the depth was 1.2-2.0 cm. The free vastus lateralis flaps were designed to repair the wounds, and skin grafting covered the vastus lateralis flaps. The length of the vastus lateralis flap was 5.0-14.0 cm, the width was 3.5-6.0 cm, and the thickness was 1.0-1.5 cm. The donor sites of the muscle flaps were directly sutured. Results The time for vastus lateralis flaps harvested ranged from 30 to 80 minutes (mean, 55.0 minutes), and the total operation time ranged from 125 to 170 minutes (mean, 147.5 minutes). All muscle flaps and skin grafts survived successfully, and the wounds and the incisions at the donor sites healed by first intention. All patients were followed up 6-24 months, with an average of 12.8 months. The appearances of 3 patients who did not follow the doctor’s instructions for pressure treatment of the muscle flaps were a little bloated, and the rest had a good appearance. The texture of the muscle flaps was soft. There were linear scars at the donor sites. There was no recurrence of ulcers during follow-up. All patients could walk independently without limitation of daily activities at last follow-up. Conclusion The application of free vastus lateralis flap combined with skin grafting to repair small- and medium-sized lacunar defects in the non-weight-bearing area of diabetic foot has the advantages of simple operation and time-saving as well as small damage to the donor site, with good repair effect, especially for the elderly patients who are not suitable for prolonged anesthesia.

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  • Preliminary application of ulnar cortex transverse transport technique in treatment of upper extremity thromboangiitis obliterans

    Objective To investigate the feasibility and effectiveness of ulnar cortex transverse transport technique in treating upper extremity thromboangiitis obliterans (TAO). MethodsA retrospective analysis was conducted on the clinical data of 7 male patients with upper extremity TAO who were admitted and met the inclusion criteria between January 2019 and July 2022. The patients ranged in age from 32 to 50 years, with a mean age of 40.1 years. The disease duration ranged from 6 to 24 months, averaging 13.6 months. The smoking history ranged from 8 to 31 years, with a mean of 18.4 years. All patients presented with finger ulcers or gangrene, including 1 case affecting the thumb and index finger, 1 case affecting the index and middle fingers, 1 case affecting the middle and ring fingers, 1 case affecting only the ring finger, and 3 cases affecting the ring and little fingers. The preoperative visual analogue scale (VAS) score was 9.1±0.7. All patients underwent treatment with the ulnar cortext transverse transport technique. The wound healing time and the disappearance time of rest pain were recorded. The VAS score was used to assess the degree of hand pain before and after operation. Serum interleukin 6 (IL-6) levels were measured before operation and at 1 month after operation. Computed tomography angiography (CTA) of the affected limb was performed before operation and at 3 months after operation to evaluate changes in blood vessels. The clinical outcomes were evaluated at 1 year after operation based on the Patwa and Krishnan grading system. ResultsAll 7 patients were followed up 12-17 months, with an average of 13.7 months. All patients experienced successful healing of ulcers, with wound healing time ranging from 14 to 21 days, averaging 17.3 days. During the follow-up, no complication occurred, and there was no recurrence of ulcers. The disappearance time of rest pain ranged from 8 to 15 days, averaging 12.1 days. The pre- and post-operative (1-month) serum IL-6 levels were (25.1±5.9) pg/mL and (11.9±2.9) pg/mL, respectively, with a significant difference (t=5.363, P=0.002). CTA examination at 3 months after operation revealed partial revascularization of upper extremity arteries and establishment of collateral circulation, showing significant improvement compared to preoperative status. The VAS scores at 1, 7, 28 days, and 6 months postoperatively were 6.4±0.8, 3.7±0.8, 0.6±0.8, and 0.1±0.4, respectively, all of which significantly improved compared to preoperative scores (P<0.05). Furthermore, the VAS scores gradually decreased over time, with significant differences observed between postoperative time points (P<0.05). At 1 year after operation, the effectiveness of all 7 patients were evaluated as excellent based on the Patwa and Krishnan grading system. ConclusionThe ulnar cortex transverse transport technique can improve blood circulation in the upper limb of patients with TAO, reconstruct microcirculation, inhibit inflammation, promote ulcer healing, and alleviate limb pain.

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  • CLINICAL OBSERVATION OF BASIC FIBROBLAST GROWTH FACTOR COMBINED WITH TOPICAL OXYGEN THERAPY IN ENHANCING BURN WOUND HEALING

    Objective To investigate the efficacy of basic fibroblast growth factor (bFGF) combined with topical oxygen therapy for deep II degree burn wounds, by comparing the effects of bFGF combined with topical oxygen therapy and bFGF with routine therapy. Methods From February 2004 to July 2009, 85 patients with deep II degree burn wounds (117 wounds) were enrolled and divided into 4 groups randomly according to different treatments. There was no significant difference in sex, age, disease course, wound size, and wound treatment size among 4 groups (P gt; 0.05). In group A, 18 patients (28 wounds) were treated routinely; in group B, 23 patients (30 wounds) were treated with routine methods and topical oxygen therapy; in group C, 19 patients (25 wounds) were treated with routine methods and bFGF therapy; and in group D, 25 patients (34 wounds) were treated with routine methods and bFGF/topical oxygen therapy. Topical oxygen therapy was administered to the wound for 90 minutes per day for 3 weeks. The bFGF therapy was appl ied everyday (150 U/ cm2) for 3 weeks. Results All cases were followed up 6-12 months (9 months on average). The wound heal ing times in groups A, B, C, and D were (27.3 ± 6.6), (24.2 ± 5.8), (22.2 ± 6.8), and (18.2 ± 4.8) days, respectively; showing significant difference between group A and group D (P lt; 0.05). The wound heal ing rates in groups A, B, C, and Dwere 67.8% ± 12.1%, 85.1% ± 7.5%, 89.2% ± 8.3%, and 96.1% ± 5.6%, respectively; showing significant differences between group A and groups B, C, D (P lt; 0.05). The therapic effective rates in groups A, B, C, and D were 75%, 90%, 92%, and 100%, respectively; showing significant difference between group A and group D (P lt; 0.05). The Vancouver scar scale scoring of group D 6 months after treatment was better than that of group A (P lt; 0.05). Conclusion The bFGF combined with topical oxygen therapy can enhance deep II degree burn wound heal ing. Furthermore, the therapy method is simple and convenient.

    Release date:2016-08-31 05:48 Export PDF Favorites Scan
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