west china medical publishers
Keyword
  • Title
  • Author
  • Keyword
  • Abstract
Advance search
Advance search

Search

find Keyword "肌腱缺损" 7 results
  • EFFECT OF TRIPTOLIDE ON ALLOGENIC TENDON TRANSPLANTATION IN REPAIRING TENDON DEFECT IN CHICKEN

    Objective Tri ptol ide can suppress immunological rejection reaction. To investigate the effect of tri ptol ide on allogenic tendon transplantation in repairing tendon defect in chicken. Methods The defect model of the third toes tendon was establ ished in 64 healthy-cleaning male Leghorn chickens (4-month-old, weighing 1.9-2.3 kg), which underwent allogenic tendon transplantation for repairing and were divided into 2 groups randomly (n=32). Tri ptol ide feeding[100 μg/(kg·d)] was given for 3 weeks in the experimental group and normal feeding in the control group. General condition of the chickens was observed after operation. The transplanted tendons were harvested from 4 chickens in each group for gross observation at 1, 2, 3, and 4 weeks after operation; the histological observation was performed at 1 and 3 weeks, and transmission electron microscope observation at 2 and 4 weeks. The blood and tendon were harvested from another 8 chickens in each group for flow cytometry and biomechanical tests respectively at 3 and 6 weeks. Results All chickens survived to the experiment end. Gross observation: with time extending, hyperemia and edema around transplanted tendon were rel ieved. Rarefaction adhering zone was seen in experimental group, and pyknotic adhering zone in control group. Histological observation: inflammatory reaction in experimental group was sl ighter than that in control group at 1 and 3 weeks. Transmission electron microscope observation: at 2 and 4 weeks, fibroblasts had big cell nucleus, more euchromatin, and l ittle heterochromatin in experimental group; however, there were small amount of rough endocytoplasmic reticulums with gentle expanded capsular space in control group, which contained sparse content. Flow cytometry test: at 3 and 6 weeks, peri pheral blood contained less CD4+ and CD8+ T lymphocytes in experimental group than in control group, and the ratio of CD4+ to CD8+ T lymphocyte significantly decreased in experimental group when compared with control group (P lt; 0.05). Biomechanical examination: at 3and 6 weeks, the maximum tensile strength in experimental group was bigger than that in control group, and tensile adhesion power in experimental group was smaller than that in control group. There were significant differences in the indexes between 2 groups (P lt; 0.05). Conclusion Tri ptol ide can suppress immunological rejection reaction, strengthen tendon healing strength, and reduce tendon adhesion in allogenic tendon transplantation.

    Release date:2016-08-31 04:23 Export PDF Favorites Scan
  • LONG-TERM EFFECTIVENESS OF TENDON ALLOGRAFT FOR REPAIRING TENDON DEFECT

    Objective To observe the long-term effectiveness of tendon allograft to repair tendon defect. Methods Between October 1996 and September 1999, 24 patients with tendon defect were treated with tendon allograft which was cultured with deoxyguanosine and preserved at low-temperature or ultra-deep-low-temperature. There were 19 males and 5 females, aged from 12 to 46 years with an average of 25.9 years. These patients included 7 cases of total extensor tendon defect of 2nd-5th fingers, 7 cases of index finger extensor tendon defect, 3 cases of deep flexor tendon defect of 2nd- 5th fingers, 1 case of ring finger deep flexor tendon defect, 3 cases of long extensor tendon defect of 2nd-5th toes, 2 cases of long extensor hallucis tendon defect, and 1 case of shoulder adduction missing. The sizes of tendon defect ranged from 5 to 15 cm. The mean time from injury to operation was 1.3 months (range, 2 hours to 3 months). Results Incisions healed by first intention. No deep infection, infectious diseases, and obvious immune rejection occurred. All patients were followed up from 10 to 12 years with an average of 10.8 years. When compared with contralateral sides, at 10 years of follow-up, 1 patient lost 6-10° flexion function; after 10.6 years, flexion tendon releasing was performed; allografted tendon had normal color and elasticity with decreased diameter and with mild and moderate adherence; and after releasing, function was improved. According to Hand Surgery Association assessment standard, the results were excellent in 12 cases, good in 6, and poor in 6; the excellent and good rate was 75%. Conclusion Tendon allograft which is cultured with deoxyguanosine and preserved at low-temperature or ultra-deep-low-temperature is safe to use in cl inical, which has good long-term effectiveness in treating tendon defect.

    Release date:2016-08-31 05:42 Export PDF Favorites Scan
  • 掌背动脉肌腱皮瓣修复手指皮肤合并肌腱缺损

    目的 总结第2、4 掌背动脉肌腱皮瓣修复手指皮肤合并肌腱缺损的临床疗效。 方法 1995 年7 月- 2010 年6 月,采用第2、4 掌背动脉肌腱皮瓣修复28 例外伤致手指皮肤合并肌腱缺损患者。男19 例,女9 例;年龄17 ~ 48 岁,平均29 岁。示指14 例,中指9 例,环指5 例。指背23 例,指腹5 例。皮肤缺损范围为2.5 cm × 2.4 cm~ 5.6 cm ×4.5 cm,肌腱缺损长度为1.3 ~ 4.8 cm。急诊手术19 例,择期手术9 例。术中皮瓣切取范围为3.5 cm × 3.4 cm ~ 6.6 cm ×5.5 cm。5 例伴指骨缺损者取自体髂骨植骨修复。供区直接拉拢缝合。 结果 术后4 d 7 例皮瓣远端出现水疱,经换药痊愈;其余皮瓣均顺利成活,创面Ⅰ期愈合。供区切口均Ⅰ期愈合。28 例均获随访,随访时间5 ~ 12 个月,平均10 个月。5 例指骨缺损者术后3 个月植骨均骨性愈合。皮瓣外形较满意,质地柔软。末次随访时肌腱功能恢复按手指总主动活动度(TAM)评定法:获优9 例,良15 例,中4 例,优良率85.7%。 结论 第2、4 掌背动脉肌腱皮瓣修复手指皮肤合并肌腱缺损具有外形及功能恢复满意的优点,是一种较好的修复方法。

    Release date:2016-08-31 05:44 Export PDF Favorites Scan
  • CLINICAL APPLICATION OF ALLOGENEIC TENDON

    Objective To explore the clinical application of allogeneic tendonhandled by ultra-deep-low-temperature. Methods From March 1995 to June 2002,tendons rupture and tendons defect were repaired by ultra-deep-low-temperatureallogeneic tendon grafts in 96 cases, including 51 cases of flexor or extensor tendon defect of hand, 36 cases of coracoclariculare ligament rupture, 4 cases of cruciate ligament rupture, 2 cases of calcaneus tendon rupture and 3 cases ofextensor tendon defect of foot. The interval between injury and operation was 1day to 5 months. The length of defect was 4-12 cm with an average of 7 cm.The matching allogeneic tendons were selected during operation, and early function exercise was done after operation. Secondary release was performed in the cases of tendon adhesion.Results Follow-up time is from 6 months to 5 years (3.4 years on average). No rejection or rupture occurred after tendon allograft. The satisfactory result was achieved in 92.71% of the cases. Conclusion The allogeneic tendon is similar to auto-tendon in transplanting, healing and function is useful clinically.

    Release date:2016-09-01 09:30 Export PDF Favorites Scan
  • 掌背复合皮瓣修复手指背部皮肤及肌腱缺损

    目的探讨掌背复合皮瓣修复手指背部皮肤及肌腱缺损的临床效果。 方法将2011年1月-2013年6月收治的手指背部皮肤及肌腱缺损患者80例按其治疗方法分为对照组和观察组,每组40例。对照组患者采用带蒂皮瓣转移治疗,观察组患者采用掌背复合皮瓣修复治疗,比较两组患者的治疗效果。 结果观察组与对照组手术时间分别为(71.9±12.6)、(147.2±15.7)min,修复出血量分别为(20.6±5.2)、(45.6±7.2)mL,患者术后住院时间分别为(2.6±0.4)、(5.0±1.2)d,差异均有统计学意义(P<0.05)。观察组和对照组总有效率分别为95.0%和85.0%,两组比较差异无统计学意义(P>0.05);满意度分别为95.0%和65.0%,两组比较差异有统计学意义(P<0.05);观察组和对照组分别有3例(7.5%)和7例(17.5%)出现不良反应,两组比较差异无统计学意义(χ2=1.829,P=0.176)。 结论临床上采用掌背复合皮瓣修复手指背部皮肤及肌腱缺损治疗效果理想,值得进一步推广应用。

    Release date: Export PDF Favorites Scan
  • Effects of cryopreservation on biological characteristics of tendon-derived stem cells in rat patellar tendon

    ObjectiveTo explore the effects of cryopreservation on the cell survival rate, cell viability, early apoptosis, migration ability, and tendon-related marker expression of tendon-derived stem cells (TDSCs) in rat patellar tendons.MethodsThe patellar tendon tissues were harvested from 12 4-month-old male Sprague Dawley rats; 12 patellar tendon tissues from 6 rats were cryopreserved (the experimental group), and the other 12 patellar tendon tissues were not treated (the control group). The patellar tendons were digested with 0.3% type I collagenase to obtain nucleated cells. The survival rate of nucleated cells was detected by trypan blue exclusion assay, and colony-forming ability by crystal violet staining. TDSCs were isolated and cultured to passage 3 (P3). The cell viability of TDSCs was detected by Alamar Blue method, the early apoptosis by Annexin V-FITC/PI assay, the cell migration ability by Transwell method, and the mRNA expressions of tendon-related markers [collagen type I (Col1α1), scleraxis (Scx), and tenomodulin (Tnmd)] by real-time quantitative PCR.ResultsThe survival rate of nucleated cells was 91.00%±3.63% in the control group, and was 61.65%±4.76% in the experimental group, showing significant difference (t=12.010, P=0.000). The formation of the primary nucleated cell clones was observed in 2 groups. At 12 days, the number of colonies forming of the experimental group [(8.41±0.33)/1 000 nucleated cells] was significantly lower than that of the control group [(15.19±0.47)/1 000 nucleated cells] (t=28.910, P=0.000). The percentage of TDSCs in the active nucleated cells in the experimental group (1.37%±0.09%) was significantly lower than that in the control group (1.67%±0.10%) (t=5.508, P=0.003). The growth trend of TDSCs (P3) in the 2 groups was consistent within 14 days. There was no significant difference in absorbance (A) value between 2 groups at each time point (P>0.05). The early apoptotic rate of TDSCs was 1.67%±0.06% in the experimental group and was 1.63%±0.06% in the control group, showing no significant difference (t=0.707, P=0.519). Under microscope, TDSCs adhered to the lower chamber of the Transwell chamber; the number of cells was 445.00±9.70 in the experimental group and was 451.50±12.66 in the control group, showing no significant difference (t=0.998, P=0.342). The relative mRNA expressions of Col1α1, Scx, and Tnmd were 3.498±0.065, 0.062±0.002, and (4.211±0.211)×10–5 in the experimental group and were 3.499±0.113, 0.062±0.001, and (4.341±0.274)×10–5 in the con-trol group, showing no significant difference (t=0.013, P=0.991; t=0.042, P=0.969; t=0.653, P=0.549).ConclusionThe survival rate of nucleated cells in cryopreserved rat tendon tissues is lower, but a large number of active TDSCs, and its cell viability, early apoptosis rate, migration ability in vitro, and cell tenogenic differentiation ability are remained.

    Release date:2017-07-13 11:11 Export PDF Favorites Scan
  • Effectiveness of groin flap with external oblique aponeurosis for tendon and skin defects of dorsal foot

    Objective To investigate the effectiveness of groin flap with external oblique aponeurosis in repair of tendon and skin defects of dorsal foot. Methods Between October 2016 and January 2020, 12 patients with compound tissue defects of the dorsal foot caused by trauma were treated. There were 9 males and 3 females, with a median age of 42 years (range, 32-65 years). The size of the skin defects ranged from 8 cm×5 cm to 12 cm×8 cm. All wounds were accompanied by extensor tendon injury, including 6 cases of extensor hallucis longus tendon defect, 5 cases of extensor digitalis longus tendon defect, and 3 cases of extensor digitalis longus tendon and extensor digitorum brevis defects. The interval between injury and admission was 1-6 hours (mean, 3 hours). After admission, the wounds were thoroughly debrided, and the groin flap with external oblique aponeurosis was used to repair the skin and tendon defects in the second stage. The size of skin flap ranged from 10 cm×6 cm to 13 cm×9 cm, and the size of the external oblique aponeurosis ranged from 5.5 cm×3.0 cm to 8.0 cm×5.0 cm. The wounds at donor sties were sutured directly. Results All flaps survived completely without significant complications. All incisions of the recipient and donor sites healed by first intention. All patients were followed up 16-24 months (mean, 18 months). The flaps were satisfactory in appearance and soft in texture. At last follow-up, 9 cases were excellent and 3 cases were good according to the American Orthopaedic Foot and Ankle Society (AOFAS) metatarsophalangeal-interphalangeal joint scale criteria. The toe function was satisfactory. The line scar was left without hernia or other morbidity on the donor site. Conclusion The groin flap with the external oblique aponeurosis can repair the tendon and skin defects of the dorsal foot, with concealed donor site, easy dissection and adjustable thinness, as well as the enough tough aponeurosis.

    Release date: Export PDF Favorites Scan
1 pages Previous 1 Next

Format

Content