west china medical publishers
Keyword
  • Title
  • Author
  • Keyword
  • Abstract
Advance search
Advance search

Search

find Keyword "肺保存" 3 results
  • 参附注射液对兔移植肺缺血再灌注损伤的保护作用

    目的 观察参附注射液对供肺缺血再灌注损伤的保护作用。 方法 将20只新西兰白兔随机分为实验组和对照组,每组各10只,建立兔左肺自体原位移植模型,分别用参附注射液和生理盐水对兔肺进行预处理和供肺灌注。于主动脉阻断前、再灌注后15 min、30 min和60 min各时点检测左肺静脉血中丙二醛(MDA)含量、总超氧化物歧化酶(SOD)的活力,于再灌注60 min后称左肺组织的干湿比重(D/W),并观察其病理变化。 结果 主动脉阻断前两组MDA含量差异无统计学意义(Pgt;0.05);再灌注15 min后实验组MDA含量较主动脉阻断前下降;再灌注30 min和60 min时,两组MDA含量均呈上升趋势,但实验组明显低于对照组(Plt;0.05)。主动脉阻断前实验组SOD活力明显高于对照组(Plt;0.05),再灌注后两组SOD活力均呈下降趋势,以对照组下降幅度明显(Plt;0.05)。实验组的D/W显著高于对照组(0.23±0.01 vs. 0.19±0.02,Plt;0.05)。对照组肺组织水肿明显,大量的炎性细胞浸润,肺泡腔内有片状渗出;而实验组表现为肺泡间隔水肿轻微,少量炎细胞浸润,渗出不明显。 结论 参附注射液对供肺的缺血再灌注损伤有较好的保护作用。

    Release date:2016-08-30 06:02 Export PDF Favorites Scan
  • Progress of Lung Preservation Solution in Lung Transplantation

    Abstract: Although lung transplantation has been established as the only valid therapeutic approach for endstage pulmonary disease, several related problems remain to be solved. In addition to the serious problem in donor lung shortage, primary graft dysfunction caused by lung ischemia-reperfusion injury is one of the most common reason of early mortality. Optimal preservation of lung is essential to reduce ischemic organ dysfunction after lung transplantation. The development of a highly reliable lung preservation solution that reduces ischemia-reperfusion injury will improve the functioning of transplanted lungs. The progress of the type, perfusing technique or strategies and modified methods of lung preservation solution are reviewed in this article.

    Release date:2016-08-30 06:15 Export PDF Favorites Scan
  • The protective effect of N-acetyl-L-cysteine on donor rat lung in the period of reperfusion

    Objective To observe the protective effect on rat lung by using N-acetyl-L-cysteine(NAC) a inhibiter of nuclear factor-kappa B (NF-κB) in the period of reperfusion. Methods Twenty-four rats were randomly divided into a control group and a trail group.The harvested lung blocks of 12 rats were flushed with and stored in the low-potassium-dextran (LPD) solution at 4℃ for 16 hours. The isolated rat lung reperfusion models were established and the donor lungs were perfused for 1 hour. NAC was used in the trail group but normal saline was used in control group. Partical pressure of oxygen in artery (PaO2), peak airway pressure (PawP) were measured at every 15 min intervals during reperfusion. After reperfusion, the lung tissue wet-to-dry(W/D)ratio, and myeloperoxidase(MPO) activity were obtained. The protein and mRNA expressions of intercellular adhesion molecule-1(ICAM-1), NF-κB were also observed by using immunohistochemistry and semi-quantitative RT-PCR at the end of reperfusion. Results The level of decreased PaO2 and increased PawP in trail group were lower than those in control group at every interval time the sample obtained after reperfusion in 60 min. (Plt;0.01 or lt;0.05). After reperfusion the W/D,MPO, the protein and mRNA expressions of ICAM-1, NF-κB were decreased evidently in trail group than those in control group(Plt;0.01 or lt;0.05). Conclusion Using NAC in the period of reperfusion, can effectively inhibit the expression of NF-κB and ICAM-1,further improve lung respiratory functions.

    Release date:2016-08-30 06:28 Export PDF Favorites Scan
1 pages Previous 1 Next

Format

Content