ObjectiveTo systematically review the correlation between RUNX3 expression of colorectal cancer and its clinical characteristics. MethodsDatabases including PubMed, The Cochrane Library (Issue 9, 2013), EMbase, MEDLINE (Ovid), CNKI, VIP, WanFang Data and CBM were searched for collecting published international and domestic case-control studies on the correlation between RUNX3 expression of colorectal cancer and its clinical characteristics from the date of their establishment to October 1st, 2013. Two reviewers screened literature according to the inclusion and exclusion criteria, extracted data, and assessed the methodological quality of the included studies. Then, meta-analysis was performed using RevMan 5.2 software. ResultsA total of 7 case-control studies were included involving 804 cases in total (521 cases in colorectal cancer group and 383 cases in control group). The results of meta-analysis showed that, RUNX3 expression was lower in the colorectal cancer group than in the normal control group (OR=0.05, 95%CI 0.03 to 0.08, P < 0.000 01); higher in the moderately/highly-differentiated group than in the poorly differentiated group (OR=4.77, 95%CI 2.88 to 7.90, P < 0.000 01); higher in colorectal cancer with invasion depth on T1-T2 stage than in that on T3-T4 stage (OR=8.13, 95%CI 4.15 to 15.92, P < 0.000 01); and lower in the colorectal cancer accompanied with lymph node metastasis than that without lymph node metastasis (OR=0.23, 95%CI 0.14 to 0.36, P < 0.000 01), all with significant differences. No significant difference was found in RUNX3 expression between groups by age, sex and tumour location. ConclusionRUNX3 expression is notably correlated to colorectal cancer and its clinical characteristics. Due to the quantity and quality limitation of the included studies, the above conclusion still needs to be further proved by performing more high quality studies.
ObjectiveTo investigate the relationship between the pathological and functional changes of the retina and the expression of monocyte chemoattractant protein (MCP)-1 after retinal laser injury in mice. MethodsA total of 116 C57BL/6 mice were randomly divided into the normal group (58 mice) and the injured group (58 mice). Retinal laser injuries were induced by Argon ion laser. At 1, 3, 7 days after laser injury, electroretinogram (ERG) responses were recorded to detect the function of the retina. Hematoxylin and eosin (HE) staining was performed to observe pathological changes. Quantitative real-time polymerase chain reaction (PCR) was performed to detect gene expression of MCP-1. Western blot was used to measure the protein expression of MCP-1. ResultsHE staining showed a progressive damage of the retinal structure. The results of ERG showed that the differences of dark-adaptive a wave (t=6.998, 9.594, 13.778) and b wave (t=12.089, 13.310, 21.989) amplitudes of 1, 3 and 7 day post-injury between normal group and injured group were statistically significant (P=0.000). At 1 day post-injury, the differences of light adaptive b wave amplitudes between the two groups were statistically significant (t=8.844, P=0.000). While the differences of light-adaptive a wave amplitudes were not (t=2.659,P=0.200). At 3, 7 days post-injury, the differences of a (t=3.076, 7.544) and b wave amplitudes (t=10.418, 8.485) between the two groups were statistically significant (P=0.000). In dark-adaptive ERG, the differences of a wave amplitudes between 1 day and 3 days (t=3.773), 1 day and 7 days (t=5.070) and b wave amplitudes between 1 day and 7 days (t=4.762) were statistically significant (P<0.01), while the differences of a wave amplitudes between the 3 days and 7 days (t=1.297) and b wave amplitudes between 1 day and 3 days (t=2.236), 3 day and 7 days (t=2.526) were not significant (P=0.660, 0.120, 0.060). In light-adaptive ERG, the differences of a wave amplitudes between 1 day and 7 days (t=2.992) and b wave amplitudes between 1 day and 3 days (t=3.570), 1day and 7 days (t=4.989) were statistically significant (P<0.05), while the differences of a wave amplitudes between 1 day and 3 days (t=0.516), the 3 days and 7 days (t=2.475) and b wave amplitudes between 3 days and 7 days (t=1.419) were not significant (P=1.000, 0.710, 0.070). Quantitative real-time PCR showed that the differences of MCP-1 gene expression at 1, 3 and 7 day post-injury between normal group and injured group were statistically significant (t=14.329, 16.861, 5.743; P<0.05). Western blot showed that the differences of MCP-1 protein expression at 1, 3 and 7 day post-injury between normal group and injured group were statistically significant (t=75.068, 54.145, 14.653; P<0.05). ConclusionIn the first 7 days after mice retinal laser injury, there are progressive pathological and functional damage of the retina, which might be correlated with MCP-1 expression.
目的 探讨乳房肿物体表定位膜对乳腺彩超结果的影响。方法 回顾性分析2010年12月至2011年1月期间，笔者所在医院乳腺门诊检查触诊阴性的180例患者共236个乳房肿物贴膜前后的彩超检查结果，分析其血运、边界、钙化及分级情况的变化。结果 贴膜后，236个肿物中有2个血运情况有变化，7个边界有变化，5个钙化情况有变化，5个分级有变化，但贴膜前后乳房肿物的血运（P=0.500）、边界（P=0.136）、钙化（P=0.082）及分级（P=0.172）变化情况比较差异均无统计学意义。结论 乳房肿物体表定位膜不影响乳房肿物的彩超检查结果。
ObjectiveTo observe the regulation of PTB-associated splicing factor (PSF) exerts on phosphatidylinositol 3 kinase (PI3K)/Akt signaling pathway in cultured retinal pigment epithelial (RPE) cells. MethodsARPE-19 RPE cells were divided into five groups including PSF overexpression (0.25, 0.50, 1.00 μg of pEGFP-C2-PSF plasmid DNA), PSF overexpression control (pEGFP-C2 empty vector DNA), PSF inhibition (0.25, 0.50, 1.00 μg of pGenesil-PSF-RNAi plasmid DNA), PSF inhibition control (pGenesil-scramble-siRNA empty vector) and sham transfected group (treated with lipofactamine 2000 reagent, but without adding plasmid DNA) groups. After transfecting with plasmid DNA, the cells were stimulated with insulin-like growth factor-1 (IGF-1). IGF-1-stimulated ARPE-19 cells were also treated with Wortmannin and /or PSF over-expression. WST-1 assay was used to detect the proliferation rates, the VEGF mRNA levels were analyzed using real time polymerase chain reaction (PCR), the levels of phosphorylation Akt and total Akt expression were measured by western blotting. ResultsAfter IGF-1 stimulation, the difference of the cell proliferation rates between PSF overexpression group, PSF overexpression control group and sham transfected group was statistically significant (F=29.728, P<0.05). The difference of the cell proliferation rates between PSF inhibition group, PSF inhibition control group and sham transfected was also statistically significant (F=14.121, P<0.05). Compared with control group, the VEGF mRNA levels was decreased in PSF overexpression group (P=0.000 3), but increased in PSF low expression group (P=0.030 9). Furthermore, overexpression of PSF could down-regulate the activation of pAkt after IGF-1 stimulation. When combined with Wortmannin treatment, the VEGF mRNA levels in PSF overexpression group was significantly lower than the control group (P<0.05). ConclusionsAfter IGF-1 treatment, PSF plays a role in suppressing the proliferation and VEGF expression in RPE cells by inactivating PI3K/Akt signaling pathway.