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find Author "葛海燕" 3 results
  • The Application of Preservation of the Ring of Pylorus to the Reconstruction of the Digestive Tract by Interposition of Jejunum Following Total Gastrectomy

    目的探讨胃底贲门癌患者行全胃切除术后消化道的重建方式。 方法总结我院1999年3月至2002年4月间采用经腹全胃切除保留幽门环间置空肠重建消化道手术的16例胃底贲门癌患者的临床资料。 结果无一例手术死亡, 无吻合口漏及狭窄, 全组患者均治愈出院。 术后半年每餐进食200~300 g, 每日3~4次, 其中蛋白质1 g/(kg·d), 总热量为2 300~3 000 kcal,餐后无胸骨后灼痛,无胆汁返流现象及排空障碍。结论严格掌握手术适应证,保留幽门环间置空肠重建消化道能起到较好的效果。

    Release date:2016-08-28 04:49 Export PDF Favorites Scan
  • Effect of aquaporin 1 on the migration of endothelial progenitor cells

    ObjectiveTo observe the effects of aquaporin 1 (AQP1) on the proliferation and migration of endothelial progenitor-endothelial progenitor cells (EPC).MethodsBone marrow cells of AQP1 wild-type (WT) (n=6) and knockout-type (KO) mice (n=6) were isolated and differentiated into EPC in vitro. Immunofluorescence was used to detect cell surface antigens to identify EPC. Live cell kinetic imaging and quantification technology, transwell migration assays, as well as scratch test were used to compare the function of EPC between AQP1 WT and KO mice.ResultsEPC culture showed that cells were initially suspended and gradually adhered to typical mesenchymal stem cells within 7 days. After cultured on special medium for endothelial cells they were adhered and differentiated, and fusiform or polygonal, paving stone-like EPC were observed around 14 days. When cultured by special medium of EPC, CD133 and CD31 were positively detected after 7 days, and CD34 and Flk-1 were positively detected after 14 days. Positive expression of AQP1 was only detected in EPC of AQP1 WT mice. Functional studies of EPC revealed there was no significant difference in the proliferation of EPC between AQP1 WT and KO group mice. Transwell assay showed that EPC migration ability of AQP1 KO mice was significantly weaker than that of WT mice. The scratch healing ability of EPC in AQP1 KO mice was significantly lower than that of WT mice.ConclusionsEPC initially shows the characteristics of stem cells and with the prolongation of culture time, EPC gradually shows the characteristics of endothelial cells. AQP1 affects the EPC migration rather than proliferation.

    Release date:2018-05-28 09:22 Export PDF Favorites Scan
  • Influence of Jejunal Interposition Pouch Reconstruction on Nutritional Condition of Patients after Total Gastrectomy

    Objective To evaluate whether jejunal interposition pouch (JIP) reconstruction is an ideal procedure of digestive tract reconstruction after total gastrectomy. Methods Ninetyfour patients after total gastrectomy had randomly divided into two groups, JIP group 42 cases and RouxenY pouch (RYP) group 52 cases. The gastrointestinal function improvement in body weight and nutritional parameters (serum albumin, hemoglobin level, and serum protein) were compared 1 year after surgery for the two groups. Results The nutritional condition of JIP group and RYP group after operation had improved (P<0.01); and the condition of JIP group with fewer symptom problems demonstrated much more better than standard RYP group (P<0.01). Conclusion JIP that could obtain partly compensatory function after total gastrectomy is an ideal reconstruction.

    Release date:2016-08-28 04:43 Export PDF Favorites Scan
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