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find Keyword "血管发生" 2 results
  • EFFECT OF SIMVASTATIN ON INDUCING ENDOTHELIAL PROGENITOR CELLS HOMING AND PROMOTING BONE DEFECT REPAIR

    Objective To investigate the effect of simvastatin on inducing endothel ial progenitor cells (EPCs) homing and promoting bone defect repair, and to explore the mechanism of local implanting simvastatin in promoting bone formation. Methods Simvastatin (50 mg) compounded with polylactic acid (PLA, 200 mg) or only PLA (200 mg) was dissolved in acetone (1 mL) to prepare implanted materials (Simvastatin-PLA material, PLA material). EPCs were harvested from bone marrow of 2 male rabbits and cultured with M199; after identified by immunohistochemistry, the cell suspension of EPCs at the 3rd generation (2 × 106 cells/mL) was prepared and transplanted into 12 female rabbits through auricular veins(2 mL). After 3 days, the models of cranial defect with 15 cm diameter were made in the 12 female rabbits. And the defects were repaired with Simvastatin-PLA materials (experimental group, n=6) and PLA materials (control group, n=6), respectively. The bone repair was observed after 8 weeks of operation by gross appearance, X-ray film, and histology; gelatin-ink perfusion and HE staining were used to show the new vessels formation in the defect. Fluorescence in situ hybridization (FISH) was performed to show the EPCs homing at the defect site. Results All experimental animals of 2 groups survived to the end of the experiment. After 8 weeks in experimental group, new bone formation was observed in the bone defect by gross and histology, and an irregular, hyperdense shadow by X-ray film; no similar changes were observed in control group. FISH showed that the male EPC containing Y chromosome was found in the wall of new vessels in the defect of experimental group, while no male EPC containing Y chromosome was found in control group. The percentage of new bone formation in defect area was 91.63% ± 4.07% in experimental group and 59.45% ± 5.43% in control group, showing significant difference (P lt; 0.05). Conclusion Simvastatin can promote bone defect repair, and its mechanism is probably associated with inducing EPCs homing and enhancing vasculogenesis.

    Release date:2016-08-31 05:49 Export PDF Favorites Scan
  • 活性氧簇对创口愈合过程中血管新生的影响

    活性氧族是一类氧衍生的代谢物,被广泛地认为是多种生理过程以及病理状态下关键的调节剂,在血管系统中主要由还原型辅酶Ⅱ氧化酶生成。慢性创口的愈合涉及2种不同形式的血管新生:血管发生(骨髓来源分化而成的循环内皮祖细胞形成)和血管生成(已存在血管局部内皮细胞的芽生而形成)。活性氧族通过对血管新生过程中所涉及的内皮祖细胞、内皮细胞和血管平滑肌细胞功能的调节,影响创口愈合。

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