Objective To investigate the effect of constitutively active Akt1 gene on rat engrafted islets in apoptosis and revascularization, and to explore potential method of gene therapy in the islet transplantation. Methods Rat islet which was transfected constitutively actived Akt1 gene via adenovirus vector using MOI=500. Thirty-six streptozotocin induced diabetic Wistar rats were divided into 3 groups complete randomly: Adv-CA-Akt1 group, Adv-LacZ group and simple transplantation group. Blood glucose and insulin were determined after operation. TUNEL was used to detect the apoptotic islet cells. HE and immunohistochemical staining of insulin were used to evaluate the histology of the islet grafts. The microvessel density (MVD) was determined by CD31 immunohistochemical staining. Results The fasting glucose level in Adv-CA-Akt1 group restored to normal 2 days after transplantation. However, in Adv-LacZ group and simple transplantation group, it reduced but still kept being hyperglycemia. And the serum insulin level was higher than other two groups ( P ＜ 0.05). Compared to simple transplantation group and Adv-LacZ group, apoptotic rate decreased 25% in Adv-CA-Akt1 group, a large number of islet grafts were seen under the capsule of the kidney, which were positively stained by insulin antibody. In the other two groups, the islet groups mass were lighter, and few positively stained by insulin antibody. MVD showed lighter positive endothelial cells stained by CD31 antibody in the other two groups than Adv-CA-Akt1 group ( P ＜ 0.05). Conclusion Constitutively activate Akt1 gene can prolong graft survival during early posttransplant period, and can accelerate the revascularization of islet grafts effectively.
Objective To analyze the international hotspots of study in age-related macular degeneration (AMD) in recent years. Methods Articles related to AMD that appeared during period January 2002-December 2010 from Pubmed database were quantitatively analyzed by software of BICOMS. PASW 18.0 statistical software used to cluster high frequency keywords of published articles. Clustering relationships of high frequency hot topics were identified. Results A total of 8529 AMD related articles were retrieved. The number of published articles in 2009 was 2.45 times greater than that of published articles in 2002. Six thousand five hundred and fourteen AMD related articles were published in the United States, England and Germany, which accounted for 76.37% of worldwide total. Fifty-two high frequency keywords were analyzed. They were clustered into 6 categories, which were identified as physiological/genetics, etiology and prevention and control, pathology and tomography and other diagnostic techniques, surgical therapy, drug therapy and photochemical therapy, and drug/laser therapy. Conclusions Recently, more attention has been paid to AMD, and the published AMD related articles has rapidly increased. The United States and European countries occupy the dominant position for the number of published AMD related articles. The international main research focus included 6 fields: basic research in AMD, etiology, pathology and diagnostic techniques, AMD prevention and control, surgery/drugs/laser and photochemical treatment of AMD.
Objective To analyze and reveal the domestic and abroad studies on polypoidal choroidal vasculopathy (PCV) by bibliometric. Methods Foreign articles were searched from American Institute of Scientific Information online database of web of science (WOS) database, Chinese articles were searched from China National Knowledge Infrastructure (CNKI) database. The age distribution, national and regional, funding agency and citation of the PCV literatures published before December 31 2012 were analyzed by software Bibexcel and SPSS 17.0. Results Totally 696 literatures were analyzed, including 440 foreign literatures and 256 Chinese literatures. The numbers of foreign literatures were gradually increased since 2000, significantly after 2007, reached the maximum at 2011. The literatures were mainly in English, the number of the literatures of China ranked 4th of all countries. The National Natural Science Foundation of China ranked No.5 of funding agency. Citations gradually increased since 2000, increased significantly after 2008; there were 30 highfrequency subjects, and hot topics were clustered into 5 categories: treatment of PCV, genetics studies of PCV, detection, diagnosis and epidemiology of PCV, relationships between PCV and macular degeneration, related cytokine of PCV. Chinese literatures increased since 2000, significantly after 2006, and reached the maximum at 2010; there were 15 literatures supported by National Natural Science Foundation of China; Citation of Chinese literatures gradually increased since 2001, and reached the maximum at 2012. There were 30 highfrequency subjects, and hot topics were clustered into 5 categories: genetics and cytokine studies of PCV, detection and diagnosis of PCV, treatment of PCV, PCV and macular diseases, PCV and vitreous hemorrhage. Conclusions Literatures of PCV were gradually increased since 2000, abroad and domestic literatures of PCV reached maximum at 2011 and 2010 respectively. The abroad research of PCV focuses on 5 categories: treatment of PCV, genetics studies of PCV, detection, diagnosis and epidemiology of PCV, relationships between PCV and macular degeneration, related cytokine of PCV. The domestic research focuses on genetics and cytokine studies of PCV, detection and diagnosis of PCV, treatment of PCV, PCV and macular diseases, PCV and vitreous hemorrhage.
【Abstract】Objective To investigate the effects of preservative temperature on pancreatic function and determine the optimal preservative temperature for pancreatic transplantation. MethodsCold pancreatic preservation was performed and a homologous male Wistar rat model of heterotopic total pancreaticoduodenal transplantation was established. The pancreas was preserved for 6 h in UW solution at specific temperatures(0 ℃, 4 ℃, 8 ℃ and 12 ℃). After preservation, pancreatic tissue was taken for histologic examination in every group. ATP and total adenine nucleotides (TAN) were determined by using high performance liquid chromatography (HPLC). Blood glucose(BG), serum amylase and lipase were measured 24 h after transplantation. And the activity of myeloperoxidase (MPO) in the pancreatic grafts was also measured at the same time. Besides, histological observation was performed. Results Microscopic studies showed that the histomorphological changes of pancreas in 4 ℃ group were less obvious than those in other groups. Tissual concentrations of ATP and TAN decreased gradually in 4 ℃ group, 0 ℃ group, 8 ℃ group, and 12 ℃ group after 6 h of preservation(PH＜0.05). The levels of BG, serum lipase and MPO increased gradually in 4 ℃ group, 0 ℃ group, 8 ℃ group, and 12 ℃ group(PH＜0.05). The activity of MPO in 4 ℃ group （1.19±0.16 U/g ）was significantly lower than that of the control group（0.26±0.09 U/g，PH＜0.05). Conclusion The temperature of 4 ℃ is most appropriate for hypothermic pancreatic preservation and can considerably alleviate cold ischemic injury of rat pancreas.
Objective To explore clinical efficacy and independent risk factors related to the recurrence of primary spontaneous pneumothorax (PSP) after video-assisted thoracoscopic surgery (VATS). Method We retrospectively anal- yzed the clinical data of 566 PSP patients by VATS in our hospital between December 2011 and June 2014 year. The patients were divided into a triple-port group(110 patients with 88 males and 22 females, aged 31.34±15.62 years) and a double-port group (456 patients with 383 males and 73 females, aged 31.46±15.65 years) by operation methods. We tried to find out the independent risk factors related to the recurrence. Results There was no statistical difference in intraoperative blood loss, postoperative drainage, drain removal, postoperative complications, postoperative length of hospital stay and rate of postoperative recurrence between the two groups (P>0.05) . However, the operation time (P<0.001) and the drainage of postoperative 48 hours (P<0.05) in the double-port group was shorter or less than that in the triple-port group. The overall recurrence rate was 1.41% (8/566) . The minors (OR=14.75, P=0.045) , no bulla type (OR=32.76, P=0.019) , and multiple bulla type (OR=15.48, P=0.013) were the independent risk factors for postoperative recurrence. Conclusions The double- port VATS technique has similar effect with triple-port VATS technique on spontaneous pneumothorax patients under- gone pulmonary bullae ligation and pleurodesis. Bulla ligation and whole mechanical pleurodesis can get better clinical efficacy and lower rate of recurrence. The minor, no bulla type, and multiple bulla type are the independent recurrence risk factors of PSP after surgery.
ObjectiveTo compare and analyze clinical effects of video-assisted thoracoscopic surgery (VATS) lobectomy and systematic lymph node harvests for peripheral non-small cell lung cancer (PNSCLC) patients between single-port (SP) and multi-port (MP) with a propensity-matched analysis. MethodsWe retrospectively analyzed the clinical data of 324 patients presented with PNSCLC and admitted in the Affiliated Hospital of Qingdao University from January 2013 through December 2015. Six-eight patients underwent single-port thoracoscopic lobectomy were as a SP group and 256 patients with multi-port thoracoscopic lobectomy. Another 68 patients were produced by a propensity-matched analysis in these 256 patients, to match with SP group as a MP group. There were 26 males and 42 females at age of 54-62 (59.3±10.3) years in the SP group. There were 32 males and 36 females at age of 50-66 (61.5±9.4) years in the MP group. Perioperative outcomes were compared between the two groups. ResultsAll operations were accomplished successfully, without conversion to thoracotomy. Most postoperative outcomes were similar in intraoperative blood loss (136.3±22.7 ml vs. 142.2±20.3 ml), conversion (4.4% vs. 7.4%), lymph node dissection number (19.9±3.5 vs. 20.0±3.0), station (7.9±2.3 vs. 8.3±2.1), postoperative drainage volume (761.4±182.3 ml vs. 736.9±176.4 ml), chest drainage duration (5.2±1.5 d vs. 5.8±1.8 d), length of hospital stay (5.5±2.0 d vs. 5.0±2.5 d), and postoperative complications (2.9% vs. 7.4%) between the two groups (P > 0.05). There were statistical differences in operation time (138.2±20.3 min vs. 126.4±22.4 min), downtrend of pain scores (P=0.03), and patients' satisfaction level (8.8±1.4 vs. 7.3±2.3, P < 0.05). Concision Single-port thoracoscopic lobectomy is not inferior to multi-port and is a safe and feasible surgical procedure for the management of PNSCLC.