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find Author "赵科研" 7 results
  • The Effect of Different Numbers of Bone Marrow Mesenchymal Stem Cells Transplanted into Rats with Pulmonary Arterial Hypertension and Their Influence on Endothelin-1 Expression

    Abstract: Objective To study the effect of different numbers of bone marrow mesenchymal stem cells(MSCs) transplanted into rats with pulmonary arterial hypertension (PAH)induced by monocrotaline(MCT)and their influence on the expression of endothelin-1(ET-1). Methods Forty healthy male Wistar rats(weight,from 180 to 250 g) were divided into four groups by random number table(n=10):group A:Wistar rats were intraperitoneally injected with MCT 60 mg/ kg, and then injected with 1×106 MSCs via the external jugular vein;group B:Wistar rats were intraperitoneally injected with MCT 60 mg/kg,and then injected with 5×105 MSCs via the external jugular vein;MCT group:Wistar rats were intraperitoneally injected with MCT 60 mg/kg, and then injected with equal amount of PBS via the external jugular vein; control group:Wistar rats were intraperitoneally injected with equal amount of saline and then injected with equal amount of PBS via the external jugular vein. Four weeks after MSCs transplantation,right ventricular systolic pressure(RVSP) and ventricular weight ratio of right ventricle/ (left ventricle+ventricular septum)were measured. Histomorphology of lung tissue was observed. Genetic expression of ET-1 in lungs and serum peptide of ET-1 were also measured. Results Four weeks after MSCs transplantation,both RVSP and ventricular weight ratio decreased significantly in rats of group Acompared with those of MCT group(RVSP:35.8±4.2 mm Hg vs. 47.2±10.1 mm Hg,P< 0.01; ventricular weight ratio:0.357±0.032 vs. 0.452±0.056,P<0.01), but these two parameters didn’t decrease significantly in rats of group B(P> 0.05). By histopathological staining, the percentage of medial wall thickness of the pulmonary arterioles was significantly less in rats of group A than that of MCT group(19.7%±3.0% vs. 26.8%±3.6%, P< 0.01). There was no statistical difference in the percentage of medial wall thickness of the pulmonary arterioles between group B and MCT group. Reverse transcriptase-polymerase chain reaction (RTase-PCR)results showed that ET-1messenger ribonucleic acid(mRNA)expression was highest in MCT group and MSCs transplantation significantly decreasedits expression in group A, while its expression was similar between group B and MCT group. The expression ofET-1 in plasma was also significantly decreased in group A than that in MCT group. Conclusion Intravenous MSCs transplantation can significantly inhibit MCT-induced PAH,and reduce both ET-1 mRNA expression in lung and ET-1 peptide level in plasma. It’s a better choice to transplant 1×106 MSCs to inhibit PAH in rats.

    Release date:2016-08-30 05:50 Export PDF Favorites Scan
  • 法洛四联症术后发生多种并发症救治成功一例

    Release date:2016-08-30 06:13 Export PDF Favorites Scan
  • Surgical Treatment of Aortic Regurgitation with Lower Ejection Fraction

    ObjectiveTo summarize the surgical experience of aortic regurgitation with lower ejection fraction (EF). MethodsWe retrospectively analyzed the clinical data of 34 patients with aortic regurgitation and lower ejection fraction received aortic valve replacement in the General Hospital of Shenyang Military Region between January 2012 and December 2013. There were 27 males and 7 females with age of 21-74 (51.03±12.06) years. All surgical procedures were performed under general anesthesia during cardiopulmonary bypass. ResultsThere was no operative mortality. Cardiopulmonary bypass time was 40-155 (60.92±22.89) minutes, aortic clamping time varied from 24 to 79 (37.12±12.61) minutes. Postoperative ventilator-assisted time was 4 to 67 (16.12±12.74) hours. The patients were discharged 8-15 (11.03±2.04) days after surgery. When discharged, EF value was 30% to 48% (41%±4%) and significantly improved compared with that before operation (P<0.01). Pulmonary artery systolic pressure varied from 33 to 50 (38.35±4.35) mm Hg and decreased significantly than that before operation (P<0.01). Left ventricular end-diastolic volume reduced to 168-380 (269.12±52.01) ml and obviously decreased than that before operation (P<0.01). ConclusionSurgical treatment can be carried out on patients with aortic insufficiency and lower EF. Treatment results are satisfactory.

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  • 动脉导管未闭合并其他心脏畸形的外科治疗

    摘要:  目的 总结动脉导管未闭合并其他心脏畸形的外科治疗经验, 以提高手术效果。 方法 2004 年11 月~2006 年12 月手术治疗动脉导管未闭合并其他心脏畸形44 例, 40 例采用正中切口, 上、下腔静脉插管, 转流前游离动脉导管, 双10 号线结扎; 对导管粗大者可在并行转流下分离导管并结扎, 4 例患者实行分期手术。 结果 本组无手术死亡。术后发生低心排血量综合征3 例, 经积极治疗治愈; 2 例发生血红蛋白尿, 均在2d 内恢复。44 例术后超声心动图复查均无再通, 无灌注肺发生。术后随访36 例, 均在3 个月~ 2 年顺利恢复, 活动正常。 结论 经胸部正中切口手术是一种简单、有效的方法, 可同时修补其他心内畸形。分离、结扎动脉导管时应熟练掌握导管及其邻近解剖关系, 根据生命体征及导管直径的大小决定是否应用并行心肺转流。

    Release date:2016-08-30 06:08 Export PDF Favorites Scan
  • Surgical Techniques and Short-term Outcomes of Endoscopic Saphenous Vein Harvesting for Coronary Artery Bypass Grafting

    ObjectiveTo investigate short-term outcomes and surgical techniques of endoscopic saphenous vein harvesting (ESVH) for coronary artery bypass grafting (CABG). MethodsClinical data of 554 patients undergoing CABG with ESVH in General Hospital of Shenyang Military between July 2009 and August 2013 were retrospectively analyzed. There were 372 male and 182 female patients with their age of 38-84(61.3±9.0) years. Clinical outcomes, postoperative lower extremity pain and edema, wound infection and healing were analyzed. ResultsDuring ESVH, 13 patients with very little subcutaneous fat received transition to skin bridge technology or partially open incision. Average number of harvested vein grafts was 1-4 (2.0±0.6), and the quality of the vein grafts was satisfactory. Postoperative lower extremity pain and edema were significantly reduced, and there was no delayed wound healing or infection. Seven patients died postoperatively, including 2 patients with perioperative myocardial infarction, 2 patients with pneumonia, 1 patient with ventricular fibrillation, 1 patient with massive cerebral infarction, and 1 patient with multiple organ dysfunction syndrome. A total of 452 patients were followed up for 120-1 460 (742.6±188.5) days. There was no late death or reintervention during follow-up. ConclusionESVH is a safe and efficacious procedure with less postoperative lower extremity pain and edema, satisfactory vein graft quality, better postoperative recovery and cosmetic results, compared with traditional fully or intermittently open wound for saphenous vein harvesting.

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  • Multiple factors analysis on the recovery of left ventricular ejection fraction in the revascularized patients with ischaemic cardiomyopathy

    Objective We probed how to predict left ventricular ejection fraction (LVEF) of the ischaemic cardiomyopathy (ICM) patients would be improved apparently after revascularization. Methods Between July 2010 and December 2015, 245 ICM patients (30%≤LVEF≤40%) with coronary bypass grafting (CABG) were retrospectively observed. Among them, 146 patients were accompanied by ischemic mitral regurgitation (IMR) (146/245, 59.6%), and 41 patients underwent mitral valvuloplasty or replacement because of more than moderate IMR. There were 13 patients early death, and other 232 patients who were followed up over 6 months were divided into two groups based on whether or not post-operative LVEF increased by 10%: a LVEF recovered group (group A, 124 patients) and a non-recovered group (group B, 108 patients). Results Preoperative NT-proBNP in the group A was significantly higher than that in the group B (P=0.036). There were less patients with myocardial infarction in the group A than that in the group B (P=0.047), and more with angina pectoris in the group A than that in the group B (P=0.024). There was no significant difference in the extent of mitral regurgitation or mitral surgery between the groups A and B (P>0.05). There were lower left ventricular end-diastolic diameter (LVEDD), left ventricular end-systolic diameter (LVESD) and left ventricular end-diastolic volume (LVEDV) in the group A than those in the group B (P<0.05). Multivariate analysis revealed that preoperative LVEDD dilated apparently and no angina pectoris existed before surgery were independent risk factors for LVEF with no recovery in the ICM patients (30%≤LVEF≤40%) after revascularization. The LVEDD of 245 patients (including 13 early deaths) was 41-71 mm. We found that the ICM patients with LVEDD ≥60 mm were more likely to signify the unfavourable prognosis (χ2=8.63, P=0.003, OR=2.21, 95% confidence interval 1.25 to 3.91). Conclusion Preoperative LVEDD dilated and no angina pectoris before surgery are independent risk factors for LVEF with no recovery in the ICM patients (30%≤LVEF≤40%) after revascularization. LVEDD≥60 mm can be regarded as the preoperative forecasting factors for the unfavourable prognosis in the ICM patients (30%≤LVEF≤40%) after revascularization.

    Release date:2018-11-27 04:47 Export PDF Favorites Scan
  • Prediction of new atrial fibrillation after off-pump coronary artery bypass grafting based on preoperative heart rate variability: A retrospective study

    ObjectiveTo study the relationship between preoperative heart rate variability (HRV) and postoperative atrial fibrillation (POAF) after off-pump coronary artery bypass grafting (OPCAB). MethodsA retrospective analysis was performed on the clinical data of 290 patients who were admitted to the Department of Cardiovascular Surgery, General Hospital of Northern Theater Command from May to September 2020 and received OPCAB. There were 217 males and 73 females aged 36-80 years. According to the incidence of POAF, the patients were divided into two groups: a non-atrial fibrillation group (208 patients) and an atrial fibrillation group (82 patients). The time domain and frequency domain factors of mean HRV 7 days before operation were calculated: standard deviation of all normal-to-normal intervals (SDNN), root mean square of successive differences, percentage difference between adjacent normal-to-normal intervals that were greater than 50 ms, low frequency power (LF), high frequency power (HF), LF/HF. ResultsThe HRV value of patients without POAF was significantly lower than that of patients with POAF (P<0.05). The median SDNN of the two groups were 78.90 ms and 91.55 ms, respectively. Age (OR=3.630, 95%CI 2.015-6.542, P<0.001), left atrial diameter (OR=1.074, 95%CI 1.000-1.155, P=0.046), and SDNN (OR=1.017, 95%CI 1.002-1.032, P=0.024) were independently associated with the risk of POPAF after OPCAB. Conclusion SDNN may be an independent predictor of POAF after OPCAB.

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