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find Author "邢智远" 2 results
  • Regulation study of ubiquitin-specific protease 39 for cell proliferation and apoptosis in colorectal cancer cells

    ObjectiveThis study is aimed to determine the expression of ubiquitin-specific peptidase 39 (USP39) protein in the colorectal cancer (CRC) tissues, and the effect of silencing USP39 gene on the cell growth and cell cycle distribution of CRC cells.Methods① The expressions of USP39 protein in CRC tissues and its paracancerous tissues were determined by immunohistochemical staining method. ② By lentiviral infection, Lv-shUSP39 (KD-1 and KD-2 group) and Lv-shCon (shCon group) were transferred into SW1116 and HCT116 cells, and cells of blank control group did not received any treatment (Con group). To determine the role of USP39 gene in cell growth, MTT assay was performed to draw growth curve, and cell cycle distribution of CRC cells in the 4 groups were determined by flow cytometer.Results① The expression of USP39 protein was higher in CRC tissues compared to adjacent tissues (P=0.007). ② For SW1116 and HCT116 cells, the cell proliferation ability of KD-1 and KD-2 groups were remarkably decreased than those in corresponding shCon and Con groups on 3, 4, and 5-day (P<0.05). ③ Flow cytometry assay showed that, the percentage of G0/G1 phase cells were decreased obviously (P<0.05), while increased significantly in percentage of G2/M phase and number of sub-G1 phase cells in KD-1 group compared with that in the Con group and shCon group of SW1116 and HCT116 cells (P<0.05).ConclusionsThe expression of USP39 protein is highly expressed in CRC tissues. Knockdowning of USP39 gene can inhibit cell proliferation and promote cell apoptosis.

    Release date:2018-12-13 02:01 Export PDF Favorites Scan
  • 经屈氏韧带入路行腹腔镜右半结肠切除术: 附1例结肠癌报道

    目的介绍经屈氏韧带入路的腹腔镜右半结肠切除术的可行性和安全性。方法回顾性分析青岛大学附属医院胃肠外科收治的1例升结肠癌并行腹腔镜下右半结肠切除术患者的手术信息,该术式优先经屈氏韧带入路进行手术操作快速、准确地进入十二指肠胰头前间隙并确定外科层面,进行胰十二指肠上方、下方和外侧Toldt间隙拓展。结果该例患者的手术Toldt间隙游离并淋巴结清扫时间55 min,整个手术时间90 min,术中出血量为约20 mL,未输血,术后无消化道出血、吻合口漏、粘连性肠梗阻等并发症发生,结合加速康复外科举措的运用,于术后第6天康复出院。结论经屈氏韧带入路是一种新的腹腔镜下右半结肠切除术的入路方式,具有解剖精准、导向清晰、操作快速、安全、符合肿瘤根治原则等列优点。

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