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find Author "陈姣" 7 results
  • 尿沉渣中相似有形成分的特征及鉴别

    目的 提高临床检验人员尿沉渣显微镜检中相似有形成分的辨认能力。 方法 通过分析2007年-2012年参加美国病理家协会和卫生部临检中心能力比对试验122份图片标本的回报结果,归纳尿沉渣相似有形成分辨认的主要错误,总结常见的尿沉渣中相似有形成分的形态特征和鉴别要点。 结果 122份样本中115份鉴定结果正确,7份鉴定错误,错误率5.7%。 结论 通过定期的学习和比较,使检验技师尿沉渣相似有形成分的识别能力得到提升,尿沉渣检验质量得到提高。

    Release date:2016-09-07 02:37 Export PDF Favorites Scan
  • Different acupuncture methods on pregnancy outcome of in vitro fertilization-embryo transfer: a network meta-analysis

    Objective To analyze the efficacy and safety of different acupuncture methods on outcome of in vitro fertilization-embryo transfer (IVF-ET). Methods The PubMed, EMbase, Cochrane Library, CNKI, VIP, WanFang Data and CBM databases were searched to collect randomized controlled trials (RCTs) related to the objectives of the study from the inception to April 16, 2023. After two investigators independently screened the literature, extracted the data and evaluated the risk of bias of the included studies, a network meta-analysis was performed using Stata 16.0 software. Results There were 62 trials total with 9844 patients, involving 7 interventions. Network meta-findings analysis revealed the following: ① Clinical pregnancy rate (CPR): needle warming > auricular acupressure > transcutaneous electrical acupoint stimulation (TEAS) > electroacupuncture > acupuncture > sham acupuncture > no adjunctive treatment; ② Live birth rate (LBR): electroacupuncture > auricular acupressure > TEAS > acupuncture > sham acupuncture > no adjunctive treatment. Conclusion Needle warming assisted IVF-ET is superior to other acupuncture therapies in improving CPR, especially during the promotion period of excretion, and the selection of Zusanli, Guanyuan and uterine acupoints for 3-month cycles may have the best effect. And for the LBR, the effect of electroacupuncture is better than that of other therapies. Besides, auricular acupressure may have good therapeutic potential. Due to the limited quality and quantity of the included studies, more high quality studies are required to verify the above conclusions.

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  • Computer-aided prediction and molecular mechanism investigation of active components in compound Kushen injection inhibiting p21-activated kinase 1

    Targeting p21-activated kinase 1 (PAK1) is a novel strategy for pancreatic cancer treatment. Compound Kushen injection contains many anti-pancreatic cancer components, but the specific targets are unknown. In this study, 14α-hydroxymatrine, an active component of Kushen injection, was found to possess high binding free energy with the allosteric site of PAK1 by molecular docking based virtual screening. Molecular dynamics simulations suggested that 14α-hydroxymatrine caused the α1 and α2 helices of the allosteric site of PAK1 to extend outward to form a deep allosteric regulatory pocket. Meanwhile, 14α-hydroxymatrine induced the β-folding region at the adenosine triphosphate (ATP)-binding pocket of PAK1 to close inward, resulting in the ATP-binding pocket in a “semi-closed” state which caused the inactivation of PAK1. After removal of 14α-hydroxymatrine, PAK1 showed a tendency to change from the inactive conformation to the active conformation. We supposed that 14α-hydroxymatrine of compound Kushen injection might be a reversible allosteric inhibitor of PAK1. This study used modern technologies and methods to study the active components of traditional Chinese medicine, which laid a foundation for the development and utilization of natural products and the search for new treatments for pancreatic cancer.

    Release date:2024-04-24 09:50 Export PDF Favorites Scan
  • TSK-11G型与B65-01型26号两种骨髓活检针行骨髓活检术的效果比较

    【摘要】 目的 比较日本TSK-11G型骨髓活检针和国产B65-01型26号骨髓活检针进行骨髓活检的效果。 方法 2010年3月-2011年5月门诊行骨髓活检术患者105例,分别用TSK-11G型和B65-01型26号骨髓活检针进行活检,比较所取得骨髓组织的长度达标率和完整性。 结果 TSK-11G型骨髓活检针所取组织质量、长度优于B65-01型26号活检针(Plt;0.05)。TSK-11G型达标92例,占87.62%,取材完整101例,占96.19%;B65-01型26号达标22例,占20.95%,取材完整88例,占83.81%。 结论 TSK-11G型骨髓活检针行骨髓活检术所取骨髓组织更长完整性更好,值得临床推广。

    Release date:2016-08-26 02:18 Export PDF Favorites Scan
  • Application of Sysmex XT-4000i hematology analyzer in cell count and cell differential count of body-fluid samples

    Objective To evaluate the value of Sysmex XT-4000i hematology analyzer in its body-fluid mode in cell count and cell differential count of pleural effusion, ascites and cerebrospinal fluid samples. Methods A total of 95 pleural effusion, ascites and cerebrospinal fluid samples were collected from patients hospitalized between May and September 2015. The samples were tested by Sysmex XT-4000i hematology analyzer (instrument method) and modified Neubauer hemocytometer (manual method) for cell count, and the results of them were compared and analyzed. Results The instrument method and the manual method had a good consistency in nuclear cell count and erythrocyte count (kappa=0.965,P< 0.001; kappa=0.988,P<0.001). There was no significant difference in the count of mononuclear cells (P> 0.05). However, there was a significant difference in the count of multiple nuclear cells (P<0.05). Conclusions Hematology analyzer in its body-fluid mode may replace manual method in cell count of pleural effusion, ascites and cerebrospinal fluids for its high precision, high efficiency and easy operation. However, cell differential count of this method needs microscopic examination assistance.

    Release date:2017-04-19 10:17 Export PDF Favorites Scan
  • 糖原染色在急性淋巴细胞白血病诊断中的临床意义研究

    目的 分析急性淋巴细胞白血病( ALL) 糖原染色( PAS) 的阳性率,与细胞免疫分型、融合基因分析结果进行比较,探索PAS在ALL诊断中的应用价值。 方法 回顾性分析我院自2010年1月-2012年5月初发ALL患者124例,统计分析其PAS染色、细胞免疫分型、断裂点丛集区基因-abesine鼠白血病基因(BCR-ABL)融合基因、外周血象及相关临床资料。 结果 50 例经细胞免疫分型诊断为早期前B型急性淋巴细胞白血病(Pro-B ALL)的患者,PAS反应阳性者30例(60%);42例经细胞免疫分型诊断为普通型急B性淋巴细胞白血病(Common-B ALL)的患者,PAS反应阳性者23例(55%);32例经细胞免疫分型诊断为急性T淋巴细胞白血(T-ALL)的患者,PAS阳性者12例(37%)。分析显示T-ALL患者PAS的阳性率明显低于Common-B ALL和Pro-B ALL的患者(P< 0.05),Common-B ALL和Pro-B ALL之间PAS阳性率差异无显著的统计学意义(P>0.05)。38 例BCR-ABL融合基因阳性的ALL患者,PAS反应阳性者18例(47%);86例BCR-ABL融合基因阴性的ALL患者,PAS反应阳性者47例(55%),BCR-ABL融合基因阳性和阴性两组比较,PAS阳性率差异无统计学意义(P>0.05)。 结论 PAS 在ALL患者有较高的阳性率,B-ALL中PAS阳性率显著高于T-ALL,PAS可作为一种经济快速的ALL诊断及免疫亚型初步诊断的辅助手段。

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  • Comparison of diagnostic significance between myeloperoxidase staining and cytoplasm myeloperoxidase in acute leukemia cells

    Objective To detect the difference between the peroxidase (POX) by cytochemical staining and cytoplasm myeloperoxidase (cMPO) by flow cytometry in acute leukemia cells, and provide a more accurate basis for the classification of leukemia. Methods The positive rate of POX in acute leukemia cells was detected by cytochemical staining. The positive rate of cMPO in acute leukemia cells was detected by flow cytometry. Then the positive rate of POX and cMPO, and the positive cells score were analyzed. Results The positive rate and the positive cells scores between POX and cMPO in acute lymphoblastic leukemia were significantly different (P<0.05), the positive rate and the positive cells scores of POX were significantly higher than those of cMPO. The positive rate between POX and cMPO in acute non-lymphoblastic leukemia (ANLL) had significant differences (P<0.05), the positive rate of cMPO was higher than that of POX; but no difference was found between POX and cMPO positive cells scores in ANLL (P>0.05). In acute myelocytic leukemia (AML)-M1 subtype, significant difference was found in the positive rate between POX and cMPO (P=0.006); cMPO positive rate was significantly higher than that of POX, but the POX positive cells score was significantly higher than that of cMPO (P=0.001). There were no significances of positive rate and positive cells score in AML-M2, AML-M3, AML-M4, AML-M5 subtypes between POX and cMPO (P>0.05). Conclusions There are not major differences between positive rate of POX and cMPO, as well as the positive cells scores in acute leukemia, especially acute myelocytic leukemia. We can choose the better method according to the actual situation and the sensitivity requirements. The two methods should be replenished by each other and used alternately.

    Release date:2017-07-21 03:43 Export PDF Favorites Scan
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