Objective To investigate the characteristics of fundus angiography with fluorescein and indocyanine green in reattachment of rhegmatogenous retinal detachment． Methods Fundus fluorescein angiography(FFA)and indocyanine green angiography(ICGA)were performed on 21 cases with simple rhegmatogenous retinal detachment postoperatively． Results (1) On the artificial ridge of 21 cases,window defect hyperfluorescence was found in FFA,choroidal vessel dilatation and choroidal filling defect of 14 cases were showed in ICGA;(2) In 6 cases cryocoagulation,fluorescein leakage was showed in FFA and ICGA,leakage of choroid was observed in late stage of ICGA;(3) In 6 cases photocoagulation,a few fluorescein leakage was demonstrated in FFA and none of this feature was found in ICGA;(4) ICGA showed slow filling of choroid of perioptic disc in 2 case,and temporo-inferiorly in 2 case. Conclusion Window defect hyperfluorescence of FFA and choroidal vessel dilatation of ICGA on the artificial ridge were the main characteristics in reattachment of rhegmatogenous retinal detachment. (Chin J Ocul Fundus Dis,20000,16:17-19)
Objective To observe the effect of hypoxia inducible factor-1alpha;（HIF-1alpha;）to the expression of cell surface adhesion molecules CD18 and the adhesion ability of leukocytes and vascular endothelial cells under early stage of diabetic retinopathy condition.Methods The human promyelocytic leukemia cell line HL60 and the rhesus choroid-retina vascular endothelial cell line RF/6A were cultured in RPMI 1640 medium-10% human serum, which was collected from the subjects of early stage of diabetic retinopathy and age-matched healthy control. The cells were cultured in 4 groups as control group (group A), diabetic group (group B), HIF-1 anti-sense oligonucleotides (ASODN) group (group C) and HIF-1 sense oligonucleotides (SODN) group (group D). The percentages of CD18 positive cell in the HL60 cell were measured by flow cytometry and mRNA in the HL60 cell by realtime reverse transcriptionpolymerase chain reaction（RT-PCR）. Results The percentage of CD18 positive cell in the group A, B, C and D was 17.06plusmn;6.01, 42.23plusmn;2.60, 25.33plusmn;3.05 and 32.40plusmn;10.57, respectively, the differences among them were significant (F=36.47,P＜0.001). Compared to the group A,the expression of CD18 mRNA in the group B,C and D was increased about 21.05plusmn;2.07、2.23plusmn;0.96 and 25.07plusmn;2.27 times,respectively, the differences among them were significant (F=180.34, Plt;0.001). The adherent rates of HL60 to RF/6A in group A, B, C and D was 0.06plusmn;0.00,0.09plusmn;0.10,0.05plusmn;0.00 and 0.07plusmn;0.01, respectively,the differences among them were significant（F=13.06，P=0.002）.Conclusion In vitro, HIF-1 could regulate the expression of CD18 by HL60, and the adhesion of HL60 to RF/6A when the cells were exposed to diabetic serum. The effects of human serum weaken with the inhibition of HIF-1 expression.HIF-1 play regulatory role in the expression of CD18 and adhesion of leukocytes and vascular endothelial cells under early stage of diabetic retinopathy condition.