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find Author "韩仲兵" 2 results
  • Effect of Melittin on collagen type II expression of rat endplate chondrocytes induced by interleukin 1β

    Objective To observe the effect of Melittin on collagen type II (Col-II) expression of rat endplate chondrocytes (EPCs) induced by interleukin 1β (IL-1β). Methods Primary EPCs from the lumbar vertebra of 4-week-old Sprague Dawley rats were culturedin vitro and identified by morphological observation, toluidine blue staining and Col-II immunofluorescence staining. Then, MTT assay was used to determine the optimal concentration of IL-1 and Melittin. Next, EPCs at passage 3 were randomly divided into 4 groups: no treatment was done in group A as control group; the optimal concentration of IL-1β, Melittin, and both IL-1β and Melittin were used in groups B, C, and D respectively. The expression of Col-II was detected by Western blot after 48 hours intervention. Results Under inverted microscope, the first generation EPCs were polygonal; cell proliferation decreased after fifth generation, and cell morphology changed into fusiform. The acidic mucosubstance in the cytoplasm (such as Aggrecan) was stained dark blue by toluidine blue. After marking Col-II by immunofluorescence, the positive expression of cytoskeleton (green fluorescence) could be observed. MTT assay showed that IL-1β and Melittin could inhibit the EPCs in a dose-dependent manner after intervention of 24 and 48 hours, and the optimal concentrations of IL-1β and Melittin intervention were 10 ng/mL and 1.0 μg/mL respectively. Compared with group A, the expression of Col-II was significantly reduced in group B, and was significantly increased in group C by Western blot assay, but there was no significant difference between group D and group A. The Col-II expression levels of groups A, B, C, and D were 0.991±0.024, 0.474±0.127, 1.913±0.350, and 1.159±0.297 respectively, showing significant difference between the other groups (P<0.05) except between group A and group D (P>0.05). Conclusion Melittin has a protective effect on endplate cartilage, and the research results provide experimental basis for the prevention and treatment of spinal degenerative disease.

    Release date:2017-04-01 08:56 Export PDF Favorites Scan
  • 游离跗外侧动脉穿支皮瓣修复手指中远节脱套伤

    目的 探讨游离跗外侧动脉穿支皮瓣修复手指中远节脱套伤的疗效。方法 2017年1月—2021年2月,采用游离跗外侧动脉穿支皮瓣纵行瓦合修复12例(12指)手指中远节脱套伤。男8例,女4例;年龄28~55岁,平均42岁。致伤原因:皮带轮绞伤4例,挤压伤2例,机器碾挫伤6例。示指7例、中指3例、环指2例。创面范围7.0 cm×5.5 cm~7.5 cm×6.0 cm,合并不同程度肌腱、骨外露及损伤。受伤至手术时间2~4 h,平均2.5 h。皮瓣切取范围为7.5 cm×6.0 cm~8.0 cm×6.5 cm。供区植皮修复。结果 术后皮瓣及供区植皮均顺利成活,创面Ⅰ期愈合。患者均获随访,随访时间6~12个月,平均8个月。皮瓣外形良好,色泽正常、无色素沉着,质地柔软;术后6个月皮瓣两点辨别觉为6~12 mm,平均9 mm。末次随访时,按中华医学会手外科学会上肢部分功能评定试用标准,获优7例、良4例、可1例,优良率达91.7%。供区足部感觉和功能无明显影响。结论 游离跗外侧动脉穿支皮瓣是修复手指中远节脱套伤的一种良好选择。

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