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find Author "马荣" 3 results
  • Effects of Survivin Antisense RNA on Apoptosis and Reversing Drug Resistance of SGC7901 Cells

    Objective To study the effects of survivin antisense RNA on SGC7901 cell’s apoptosis and chemosensitivity to taxotere, and to investigate its effect on the expression of multi-drug resistance gene-1 (MDR-1). Methods Survivin antisense eukaryotic vector anti-pcDNA3-svv was transfected into SGC7901 cell lines by lipofectamine and positive clones were screened out then. Survivin protein and MDR-1 mRNA were measured by western blot and RT-PCR, respectively. Apoptosis that was induced by anti-pcDNA3-svv was observed by electronic microscope, and the sensitivity of SGC7901 cell to taxotere was examined by MTT. Results The expressions of survivin protein and MDR-1 mRNA in transfected SGC7901 cells both decreased more significantly than that of non-transfected cells (P<0.05, P<0.01), and the indices of MDR of transfection group and non-transfection group were 0.196±0.013 and 3.126±0.019, respectively, at the late phase of apoptosis, which had a significant difference between each other (P<0.01), IC50 of the transfected cells to taxotere was (16.7±1.98) ng/ml and that of the non-transfected cells was (55.7±1.89) ng/ml, which also had a significant difference (P<0.01). Conclusion Surivivin antisense RNA could induce the apoptosis of SGC7901 cancer cell line and could increase the cells’ sensitivity to taxotere, which may help to reverse drug resistance.

    Release date:2016-08-28 04:08 Export PDF Favorites Scan
  • MDT discussion of a case of solid pseudopapillary tumor of pancreas

    ObjectiveTo improve recognization and effect of surgical treatment for solid pseudopapillary tumor of pancreas (SPTP) through multidisciplinary team (MDT) discussion.MethodsThe clinical data of 1 case of SPTP treated in The Second Affiliated Hospital of Chongqing Medical University were discussed by the MDT. The best operation scheme was selected and the patient was followed-up.ResultsThe preoperative CT scan showed that the occupation on the body and tail of pancreas and suggested it was the tumorous lesions. After the MDT discussion, the preoperative diagnosis was still not completely clear. However, the decision was made to resect occupation. After the operation, the pathological examination showed it was the SPTP. There was no recurrence or metastasis with the follow-up of 6 months.ConclusionsPreoperative diagnosis of SPTP is difficult, especially for atypical SPTP. Through MDT discussion, it can help to reduce misdiagnosis rate and formulate optimal surgical treatment strategy.

    Release date:2019-09-26 10:54 Export PDF Favorites Scan
  • 经皮经肝胆道镜碎石取石术治疗胆肠吻合术后肝胆管结石病

    目的探讨经皮经肝胆道镜碎石取石术(PTCSL)必要时联合球囊扩张治疗胆肠吻合术后肝胆管结石病的可行性及安全性。方法重庆医科大学附属第二医院 2015 年 12 月至 2020 年 12 月期间共有 54 例胆肠吻合术后肝胆管结石病患者接受 PTCSL 必要时联合球囊扩张治疗(符合纳入标准)。回顾性收集患者的一般资料、手术情况、术后情况以及术后随访资料。结果54 例患者中有 52 例(96.3%)行 PTCSL 治疗成功,2 例因为胆肠吻合口完全闭塞而失败。术中出血量的中位数为 55 mL(15~520 mL);取净结石 48 例(88.9%),结石残留 6 例(11.1%)。术后并发症发生率为 27.8%(15/54),无围手术期死亡患者。取净结石的 48 例患者获随访,中位随访时间 33 个月(2~60 个月),有 10 例结石复发,复发率为 20.8%(10/48)。结论PTCSL 必要时联合球囊扩张治疗胆肠吻合术后肝胆管结石病具有良好的安全性和可行性,但是术后需要注意预防结石复发。

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