west china medical publishers
Author
  • Title
  • Author
  • Keyword
  • Abstract
Advance search
Advance search

Search

find Author "高尚志" 5 results
  • 结肠代食管术的临床研究进展

    摘要:结肠代食管的价值已被众多学者肯定,但因该手术操作技术复杂,术后并发症发生率高,一旦发生移植结肠段坏死,死亡率极高,故此手术未能广泛开展.近年来,众多文献报道对此手术的技术革新,其并发症和死亡率大大下降.因此,对结肠代食管术的适应证、手术技术、并发症的防治以及术后生活质量的新近认识进行了综述.

    Release date:2016-08-30 06:35 Export PDF Favorites Scan
  • The Role of Protein Kinase C in Immature Myocardial Ischemic Preconditioning

    Abstract: Objective To investigate the mechanism of protein kinase C(PKC) in immature myocardial ischemic preconditioning in order to further its clinical applicability. Methods Langendorff perfusion heart models of 24 rabbits were set up and they were randomly divided into 4 groups: ischemic reperfusion group (I/R group), myocardial ischemic preconditioning group (MIP group), chelerythrine group (CLT group) and protein kinase C group (PKC group). The emodynamics, biochemistry and myocardial ultrastructure were observed. Results The heart function recovery and myocardial water content in the MIP and the PKC groups were better than those of the I/R and the CLT groups (Plt;0.01). The adenosine triphosphate (ATP) content, superoxide dismutase activity, mitochondrial Ca2+-ATPase activity and synthesizing ATP activity of mitochondria in the MIP and the PKC groups were significantly higher than those of the I/R and the CLT groups (Plt;0.01). The dehydrogenase and creatine kinase leakage, malondialdehyde content, myocardial cell Ca2+ content and mitochondrial Ca2+ content in the MIP and the PKC groups were significantly lower than those of the I/R and the CLT groups (Plt;0.01). The myocardial ultrastructure injuries in the MIP and the PKC groups were less than that of the I/R and the CLT groups. Conclusion Myocardial ischemic preconditioning plays an important role in protecting immature myocardium, which is probably realized by the activation of PKC.

    Release date:2016-08-30 06:02 Export PDF Favorites Scan
  • The Role of Mitochondrial Adenosine Triphosphatesensitive Potassium Channel in Immature Myocardial Ischemic Preconditioning

    Objective To investigate the role of mitochondrial adenosine triphosphatesensitive potassium channel(mitoKATP) in immature myocardial ischemic preconditioning, and to provide evidence for immature myocardial protection. Methods Langendorff isolated heart infused model was used in the experiment. Twentyfour rabbits (aged from 14 to 21 days) were randomly divided into 4 groups:ischemiareperfusion group(I/R group), myocardial ischemic preconditioning group(E1 group), 5hydroxydecanoate(5-HD) group (E2 group) and Diazoxide (Diaz) group(E3 group). Hemodynamics recovery rate, myocardial water content(MWC), the leakage rates of serum creatine kinase and lactate dehydrogenase, adenosine triphosphate content, superoxide dismutase activity, malondialdehyde content, myocardial cell Ca2+ content and myocardial mitochondrial Ca2+ content, myocardial mitochondrial Ca2+-ATPase activity, the adenosine triphosphate(ATP) synthesizing ability of myocardial mitochondria were tested, and myocardial ultrastructure was observed via electron microscopy. Results The hemodynamics recovery rate, myocardial water content(P<0.05), adenosine triphosphate content, superoxide dismutase activity, myocardial mitochondrial Ca2+-adenosine triphosphyatase(ATPase) activity and the ATP synthesizing ability of myocardial mitochondria of the rabbits in E1 and E3 group were significantly better than that in I/R group and E2 group(P<0.05). Malondialdehyde content, the leakage rates of serum creatine kinase and lactate dehydrogenase, myocardial cell Ca2+ content and myocardial mitochondrial Ca2+ content of the rabbits in E1 group and E3 group were significantly lower than that in I/R group and E2 group (P<0.05). The myocardial ultrastructure injury in E1 and E3 group were significantly reduced compared with that in I/R and E2 group. Conclusion Myocardial ischemic preconditioning has significant protective effects on immature myocardium. Its mechanism may be related to the activation of mitoKATP.

    Release date:2016-08-30 06:05 Export PDF Favorites Scan
  • Preservation Effect of Immature Myocardium with Renal Ischemic Preconditioning in Neonatal Rabbits

    Objective To investigate the immature myocardial protection effects with renal ischemic preconditioning. Methods 18 neonatal rabbits were randomly divided into three groups. Ischemic/reperfusion(I/R) group underwent 45 min ischemia followed with 45 min reperfusion after Langendorff model performed. Cardiac ischemic preconditioning(CIP) group underwent 45 min ischemia followed with 45 min reperfusion after 5 min ischemia and then 5 min reperfusion for two times. Renal ischemic preconditioning(RIP) group underwent 45 min ischemia followed with 45 min reperfusion after renal artery obstruction for 5 min and 5 min reperfusion for three times.The left ventricular function recovery,myocardial water content(MWC), lactate dehydrogenase (LDH) and creatine kinase(CK) leakage, malondialdehyde(MDA) content,adenosine triphosphate(ATP) content, superoxide dismutase(SOD) activity, myocardial cell Ca2+ [Ca2+]c content,mitochondrial Ca 2+ content [Ca2+]m,synthesizing ATP activity of mitochondria [ATP]m and Ca2+ATPase activity of mitochondria [Ca2+ATPase]m were tested. Results The recovery of postischemic heart function in RIP group and CIP group were higher than that I/R group(Plt;0.01). There were no significant difference of HR, AF in three groups (Pgt;0.05). There were significant difference of CF,CO,LVSP and LVEDP in RIP group and CIP group than those I/R group(Plt;0.01). There were significant difference of MWC, CK, LDH, ATP content, MDA, SOD activity, [Ca2+]c content, [Ca2+ATPase]m, [Ca2+]m and [ATP]m in RIP group than those I/R group(Plt;0.01). There were no significant difference between RIP group and CIP group upon every index (Pgt;0.05). Conclusion RIP has the same cardioprotection to immature myocardium as ischemic preconditioning.

    Release date:2016-08-30 06:08 Export PDF Favorites Scan
  • 大网膜移植治疗食管感染、穿孔

    目的 探讨食管感染、穿孔的手术治疗方法,以提高其成功率,降低死亡率. 方法 1970~1999年收治食管感染、穿孔患者32例,其中食管异物22例,外伤5例,食管溃疡穿孔3例,医源性损伤2例.所有患者共施行手术56例次.手术方式包括除去异物、胸腔引流、食管破口修补、邻近组织覆盖、带蒂大网膜包绕、病变食管切除、重建食管、胃造口和空肠造口. 结果 治愈26例,好转2例,死亡4例.随访1~5年,22例能进普通饮食. 结论 食管感染、穿孔治疗的关键为去除病因、清除病灶内失活组织、控制感染、胃造口减压、空肠造口供给营养和带蒂大网膜包绕食管.

    Release date:2016-08-30 06:31 Export PDF Favorites Scan
1 pages Previous 1 Next

Format

Content