west china medical publishers
Author
  • Title
  • Author
  • Keyword
  • Abstract
Advance search
Advance search

Search

find Author "黄云超" 22 results
  • aPKC-ι在非小细胞肺癌组织中的表达及其与预后的关系

    目的 研究非典型蛋白激酶C-ι(aPKC-ι)在非小细胞肺癌(NSCLC)组织中的表达,探讨其表达与NSCLC各临床病理因素及其预后的关系。 方法 采用免疫组织化学染色法检测80例NSCLC癌组织和20例肺良性疾病组织中aPKC-ι蛋白的表达情况,并随访所有患者的生存时间。 结果 NSCLC组织中aPKC-ι蛋白表达较肺良性疾病组织明显增高(P=0.006),aPKCι表达与性别(P=0.806)、组织学类型(P=0.140)差异无统计学意义,与淋巴结转移(P=0.003)、病理分期(P=0.000)、分化程度(P=0.006)差异有统计学意义;生存分析显示:aPKC-ι表达与预后明显相关(r=0.914)。 结论 aPKC-ι的表达强弱与NSCLC的发生及发展有关,可作为NSCLC患者预后参考指标,有利于个体化治疗。

    Release date:2016-08-30 05:59 Export PDF Favorites Scan
  • The Effect of Brominated Furanones on the Biofilm Formation of Staphylococcus Epidermidis on the Polyvinyl Chloride Material

    Objective To study the influence of brominated furanones on the biofilm (BF) formation of Staphylococcus epidermidis (SE) on polyvinyl chloride(PVC) materials, and provide new ideas for the research of surface modification of materials and clinical treatment of biomaterial centered infection. Methods We chose three kinds of brominated furanone with representative chemical structure for our research which were respectively 3,4dibromo-5-hydroxy2 (5H) -furanone (Mucobromic acid) in the first furanone group, 4-bromo-5(4-methoxyphenyl)3(methylamino)2(5H)furanone in the second furanone group, and 3,4dibromo-5,5-bis(4-methylphenyl)2(5H)-furanone in the third furanone group. The PVC material soaked with 75% ethanol for 5minutes was classified as the control group. The surface coating of the PVC materials in the four groups all underwent modification respectively and then they were cocultivated with staphylococcus epidermidis together. Confocal laser scanning microscope(CLSM) was adopted to detect the thickness of bacterium BF and bacterium community quantity unit area on PVC materials and scanning electron microscope(SEM) was used to observe surface structure of SE, BF formation at 6 h, 12 h, 18 h and 24 h respectively. Results The results of CLSM showed that, compared with the control group, SE bacterium community quantity unit area and the thickness of bacterium BF on the PVC material surface in the second furanone group were obviously smaller (Plt;0.05). SE bacterium community quantity unit area and thickness of bacterium BF on PVC material surface in the first and the third furanone groups had no significant difference (Pgt;0.05). The result of SEM showed that, the quantity of SE bacterium community unit area on PVC material surface in the second furanone group were smaller than that of the control group at 6 hours. The biofilm structure on PVC material surface in the control group was formed at 18 hours, but there were no mature biofilm structure on PVC material surface in the second furanone group at 18 hours. Conclusion The impact of different brominated furanone on SE biofilm formation on the surface of PVC materials is different. The second kind of furanone can inhibit the quantity of SE bacterium community unit area and SE biofilm formation on the surface of PVC materials.

    Release date:2016-08-30 06:02 Export PDF Favorites Scan
  • 原发性气管肿瘤外科治疗27例

    Release date:2016-08-30 06:06 Export PDF Favorites Scan
  • 甲状腺肿致双侧乳糜胸、乳糜心包积液一例

    Release date:2016-08-30 06:23 Export PDF Favorites Scan
  • EFFECT OF INFECTION ON CAPSULE FORMATION AFTER BREAST IMPLANTS

    ObjectiveTo investigate the correlation between infection and capsular contracture by observing the effect of infection on the formation of the surrounding capsule after breast implants. MethodsThree healthy adult female Diannan small-ear pigs underwent augmentation mammaplasty using miniature implants, which were randomly divided into group A (12 nipples), group B (10 nipples), and group C (12 nipples). Staphylococcus epidermidis (SE ATCC12228 and SE RP62A, 1.2×105 CFU/mL) was inoculated into the periprosthetics of groups B and C, and sterile PBS in group A before breast implants. Then the silica gel prosthesis was put, total 34 implants in 3 groups. After 13 weeks, the capsule was harvested to measure the capsular tension and weight. HE staining was used to observe the structure characteristics of the capsule and to measure the capsule thickness, Van-Gieson (VG) staining to observe the capsule collagen characteristics, and α-smooth muscle actin (α-SMA) immunocytochemistry staining to observe myofibroblasts in capsule. ResultsPrimary healing of incision was obtained, and 3 small-ear pigs showed stable life indication. The complete fibrous capsule was observed after 13 weeks in 3 groups. Capsule tension showed no significant difference among 3 groups (P>0.05). Capsule weight was significantly greater in group C than in groups A and B (P<0.05). HE staining showed that capsule structure of the 3 groups was similar with obvious dense layer and loose layer, and the capsule thickness was also significantly greater in group C than in groups A and B (P<0.05), but no significant difference was found between groups A and B (P>0.05). VG staining showed that collagenous fiber in the capsule were more compact in group C than in groups A and B. The α-SMA immunocytochemistry staining indicated the myofibroblasts in capsule were the most in group C. ConclusionInfection after breast implants has obvious impacts on the formation of the capsule, and there was a causal link between infection and capsular contracture.

    Release date: Export PDF Favorites Scan
  • Research progress in diagnosis and treatment of multiple primary lung cancer

    With the changes in the disease spectrum and the advancement of examination technology, the detection rate of multiple primary lung cancers (MPLC) is gradually increasing when multiple nodules and masses in the lung are examined clinically. MPLC has significant distinction with other types of lung diseases or lung cancers in the treatment and prognosis. In most cases, patients would be recommended to undergo the surgery as soon as possible which means that the accurate diagnosis should be made before surgery or during treatment. The newly developed molecular and genomic methods are more likely to better determine the relationship between multiple lesions. Artificial intelligence can be used as a related diagnostic aid to show more accurate and objective results in the diagnosis of multiple pulmonary nodules. This review summarizes the latest MPLC diagnostic research (including pathological analysis, imaging), analyzes surgical treatment methods, and looks forward to the future research direction of MPLC diagnosis and treatment, in order to provide reference for MPLC research.

    Release date: Export PDF Favorites Scan
  • Research progress on the correlation between imaging characteristics and pathological invasion degree of early lung adenocarcinoma

    With the development of multi-slice spiral computed tomography (CT) technology and the popularization of low-dose spiral CT screening, more and more adenocarcinomas presenting ground-glass nodule (GGN) are found. Pathological invasiveness is one of the important factors affecting the choice of treatment strategy and prognosis of patients with early lung adenocarcinoma. Imaging features have attracted wide attention due to their unique advantages in predicting the pathologic invasiveness of early lung adenocarcinoma. The imaging characteristics of GGN can be used to predict the pathologic invasiveness of lung adenocarcinoma and provide evidence for clinical decisions. However, the imaging parameters and numerical values for predicting pathologic invasiveness are still controversial, which will be reviewed in this paper.

    Release date: Export PDF Favorites Scan
  • BIOFILM AND ROLE OF icaA, icaD, AND ACCUMULATION-ASSOCIATED PROTEIN IN STAPHYLOCOCCUS EPIDERMIDIS ISOLATED IN BREAST SURGERY

    ObjectiveTo investigate biofilm formation on the surface of silica gel by breast surgery clinical specimens of Staphylococcus epidermidis and to analyze the relationship between biofilm formation and icaA, icaD, and accumulation-associated protein (aap) gene. MethodsBetween December 2011 and January 2013, 44 strains of Staphylococcus epidermidis were isolated from the clinical specimens of the female patients who had no symptom of infection. The icaA, icaD, and aap genes were detected by PCR and 4 genotypic groups were divided:icaA+icaD+/aap+ group (group A), icaA+icaD+/aap- group (group B), icaA-icaD-/aap+ group (group C), and icaA-icaD-/aap- group (group D). Biofilms mass was semi-quantified by semi-quantitative adherence assay after 8, 12, 24, 30, and 36 hours of incubation. The thickness of biofilms was measured by confocal laser scanning microscope (CLSM) at 12 and 24 hours after incubation. The ultrastructure of biofilms was observed by scanning electron microscope (SEM) at 24 hours after incubation. ResultsPCR test showed that 13 strains were icaA+icaD+/aap+(group A), 12 strains were icaA+icaD+/aap-(group B), 16 strains were icaA-icaD-/aap+(group C), and 3 strains were icaA-icaD-/aap-(group D). In 29 strains which had bacterial biofilm formation (65.9%), there were 13 strains in group A, 7 strains in group B, 9 strains in group C, and 0 in group D. The result of semi-quantitative adherence assay showed no significant difference in the absorbance (A) values among 4 groups at 8 hours (P>0.05). The A values of groups A, B, and C were significantly higher than that of group D at 12-36 hours, and group A was significantly higher than groups B and C (P<0.05), but there was no significant difference between groups B and C (P>0.05). The results of CLSM showed that the thickness of biofilm in groups A, B, and C was significantly larger than that in group D at 12 and 24 hours after incubation (P<0.05), and the thickness of biofilm in group A was significantly larger than that in groups B and C (P<0.05), but there was no significant difference between groups B and C (P>0.05). The result of SEM showed that the mature biofilm could be observed on the surface of silica gel in groups A, B, and C, and the ultrastructure of biofilms in group A were the most abundant and extensive among 3 groups. The ultrastructure of biofilm in group B was similar to that in group C. No obvious biofilms formed in group D. ConclusionicaA, icaD, and aap genes all play key roles in the process for biofilm formation of Staphylococcus epidermidis. Futhermore, aap gene enhance the ability of biofilm-forming when aap and ica genes coexist, so the biofilm-forming ability of icaA+icaD+/aap+ is strongest.

    Release date: Export PDF Favorites Scan
  • 单孔胸腔镜下常规手术器械替代一次性手术器械对肺癌肺叶切除术的临床分析

    目的探讨单孔胸腔镜下常规手术器械替代一次性手术器械对肺癌肺叶切除术近期临床疗效及治疗费用差异。方法前瞻性纳入 2017 年 10 月至 2018 年 6 月云南省肿瘤医院胸外一科Ⅰa~Ⅲa 期非小细胞肺癌患者 44 例。患者按手术方式不同分为一次性器械组 24 例[A 组,对照组,男 10 例、女 14 例,平均年龄 53.8(30~77)岁]和常规器械替代组 20 例[B 组,试验组,男 9 例、女 11 例,平均年龄 53.6(35~70)岁]。比较两组临床效果。结果两组手术均顺利完成,两组术中出血量、术后引流管引流量、术后胸腔引流管留置时间、术后住院时间、并发症发生情况差异无统计学意义(P>0.05)。A 组手术时间和住院总费用分别为(57.29±7.52)min 和(52 830.79±6 621.71)元,B 组手术时间和住院总费用分别为(79.00±9.81)min 和(29 565.90±2 734.25)元,两组差异有统计学意义(P<0.05)。结论单孔胸腔镜下常规手术器械替代一次性器械在肺癌肺叶切除术中安全可行,且大大降低了患者住院总费用。

    Release date:2019-07-17 04:28 Export PDF Favorites Scan
  • EFFECT OF IATROGENIC STAPHYLOCOCCUS EPIDERMIDIS INTERCELLAR ADHESION OPERON ON FORMATION OF BACTERIAL BIOFILM ON SURFACE OF POLYVINYL CHLORIDE

    Objective The intercellular adhesion (ica) gene of Staphylococcus epidermidis (SE) is a key factor to bacterial aggregation, to analysis the genotype of iatrogenic SE and to explore the effect of iatrogenic SE ica operon on theformation of bacterial biofilm on the surface of polyvinyl chloride (PVC). Methods Fifty-six cl inical isolates of iatrogenic SEwere selected, and PCR and gene sequencing were used to detect the genes related with bacterial biofilm formation. The genes contained 16S rRNA, autolysin (atlE), fibrinogen binding protein (fbe), and icaADB. The bacteria suspension of 1 × 105 cfu/mL iatrogenic SE was prepared; according to the test results of target genes, the PVC material and the genotype of icaADB+, atlE+, fbe+ strains were co-cultivated as the ica positive group; the PVC material and the genotype of icaADB-, atlE+, fbe+ strains were co-cultivated as the ica negative group. The thickness of biofilm and bacterial community quantity unit area on PVC materials were measured by confocal laser scanning microscope, and the surface structure of biofilm formation was observed by scanning electron microscope (SEM) at 6, 12, 18, 24, and 30 hours. Results The positive rate of 16S rRNA of iatrogenic SE strains was 100% (56/56). The genotype of icaADB+, atlE+, and fbe+ strains accounted for 57.1% (32/56). The genotype of icaADB-, atlE+, and fbe+ strains accounted for 37.5% (21/56). The sequencing results showed that the product sequences of 16S rRNA, atlE, fbe, and icaADB were consistent with those in GenBank. With time, no significant bacterial biofilm formed on the surface of PVC in ica operon negative group. But in ica operon positive group, the number of bacterial community was gradually increased, and the volume of bacterial biofilms was gradually increased on the surface of PVC. At 24 hours, mature bacterial biofilm structure formed, and at 30 hours, the volume of bacterial biofilms was tending towards stabil ity. The thickness of biofilm (F=6 714.395, P=0.000) and the bacterial community quantity unit area on PVC materials (F=435.985, P=0.000) in ica operon positive groupwere significantly higher than those in ica operon negative group. Conclusion Iatrogenic SE can be divided into 2 types ofica operon negative and ica operon positive bacteria. The iatrogenic SE ica operon can strengthen bacterium biofilm formation capabil ity on PVC materials, bacterium community quantity, and thickness of biofilm, it plays an important role in bacterium biofilm formation on PVC materials.

    Release date:2016-08-31 05:43 Export PDF Favorites Scan
3 pages Previous 1 2 3 Next

Format

Content