Objective To investigate the association between polymorphism of S2 locus allele in ADAM33 gene and bronchial asthma in Xinjiang Uygur population.Methods PCR-RFLP was used to determine polymorphismof S2 locus allele in ADAM33 gene in 131 Uygur patients with bronchial asthma ( asthma group) and 90 Uygur healthy individuals ( control group) .Results The comparison of three genotypes and allele frequency of the S2 in the ADAM33 gene had statistical significance in the asthma group and the control group ( X2 =6. 065, P lt;0. 05;X2=5. 255, P lt;0. 025) . The G allele of S2 site increased the risk of asthma( OR =1. 616, P lt;0. 05) . The CG genotype also increased the risk of asthma ( OR= 1. 351,P lt;0. 05) . The FEV1% pred and FVC% pred had significant difference between three genotypes of the S2 site in the ADAM33 gene in the asthma group ( F = 6. 248, P lt; 0. 01; F = 7. 067, P lt; 0. 01) .Conclusion The polymorphism of the S2 site in the ADAM33 gene has significant correlation with asthma in Xinjiang Uygur population, and can increase risk of asthma in the Uygur population.
ObjectiveTo systematically review the association between ADAM33 (a disintegrin and metalloproteinase domain33) T2 (rs2280090), S2 (rs528557), and V4 (rs2787094) polymorphisms and asthma in Chinese Han population. MethodsWe electronically searched databases including PubMed, Web of Science, CNKI, WanFang Data and VIP from inception to December 2014, to collect case-control studies about the association between ADAM33 polymorphisms and asthma in Chinese Han population. Two reviewers independently screened literature, extracted data and assessed the risk of bias of included studies. Then, meta-analysis was performed using Stata 12.1 software. ResultsA total of 8 case-control studies including 1651 asthma patients and 1639 controls were included. The results of meta-analysis showed that: ADAM33 T2 polymorphism was associated with decreased risk of asthma (AA+AG vs. GG: OR=0.316, 95%CI 0.151 to 0.659, P=0.002), and the S2 polymorphism was associated with increased risk of asthma (GG+GC vs. CC: OR=1.271, 95%CI 1.023 to 1.578, P=0.030). However, no significant association was found between asthma risk and V4 polymorphism (GG+GC vs. CC: OR=1.561, 95%CI 0.638 to 3.823, P=0.330). Subgroup analysis by age indicated that the T2 polymorphism was associated with decreased risk of asthma in both adults and children, the S2 polymorphism was only associated with increased risk of asthma in adults, however, no significant association was found between asthma and the ADAM33 V4 polymorphism in both adults and children subgroups. ConclusionCurrent evidence shows that the ADAM33 T2 and S2 polymorphisms are associated with risk of asthma in Chinese Han population. Due to the limited quantity and quality of the studies, the above conclusion still need to be verified by further more high-quality studies.