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find Keyword "Blue-SAb Rabbit" 1 results
  • THE RAPID IMMUNOSTAINING APPROACH TO THE IDENTIFICATION OF THE NATURE OF ALIVE NERVE FASCICULI

    Six New Zealand white rabbits were under the pentobarbital anesthesia, the sciatic nerve trunk were exposed and divided. Afterwards, both the proximal and distal ends of the divided nerve trunk were incubated with Blue-SAb in the micropipe for 30 minutes at room temperature. The sensory fascieuli which were bly Blue-immunostained among unstained fasciculi and other tissues had been seen under the magnifying operating microscope (×4). The neural cells of the spinal cord and the ganglion were cultured on RPMI 1640 medium containing Bright- Blue.The growth and the metabolism of the neural cells were tested by MTT method. The t-test was used to determine the statistical difference blue staining of the neural cells was considered to be of no statistical significance (Pgt;0.05).

    Release date:2016-09-01 11:33 Export PDF Favorites Scan
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